Fluorescent proteins for single-molecule fluorescence applications

Seefeldt B, Kasper R, Seidel T, Tinnefeld P, Dietz K-J, Heilemann M, Sauer M (2008)
Journal of Biophotonics 1(1): 74-82.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single-molecule applications, demonstrated by high photostability and rare fluorescence-intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single-molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications. [GRAPHICS] mCherry exhibits a relatively high photostability and brightness under single-molecule fluorescence imaging conditions. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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Zeitschriftentitel
Journal of Biophotonics
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1
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1
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74-82
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Seefeldt B, Kasper R, Seidel T, et al. Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics. 2008;1(1):74-82.
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K. - J., Heilemann, M., & Sauer, M. (2008). Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics, 1(1), 74-82. doi:10.1002/jbio.200710024
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K. - J., Heilemann, M., and Sauer, M. (2008). Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics 1, 74-82.
Seefeldt, B., et al., 2008. Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics, 1(1), p 74-82.
B. Seefeldt, et al., “Fluorescent proteins for single-molecule fluorescence applications”, Journal of Biophotonics, vol. 1, 2008, pp. 74-82.
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K.-J., Heilemann, M., Sauer, M.: Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics. 1, 74-82 (2008).
Seefeldt, Britta, Kasper, Robert, Seidel, Thorsten, Tinnefeld, Philip, Dietz, Karl-Josef, Heilemann, Mike, and Sauer, Markus. “Fluorescent proteins for single-molecule fluorescence applications”. Journal of Biophotonics 1.1 (2008): 74-82.

21 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Fluorescence lifetime based bioassays.
Meyer-Almes FJ., Methods Appl Fluoresc 5(4), 2017
PMID: 28660853
Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes.
Itoh H, Arai S, Sudhaharan T, Lee SC, Chang YT, Ishiwata S, Suzuki M, Lane EB., Chem Commun (Camb) 52(24), 2016
PMID: 26977478
A Guide to Fluorescent Protein FRET Pairs.
Bajar BT, Wang ES, Zhang S, Lin MZ, Chu J., Sensors (Basel) 16(9), 2016
PMID: 27649177
Ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches.
Marchetti L, Luin S, Bonsignore F, de Nadai T, Beltram F, Cattaneo A., Int J Mol Sci 16(1), 2015
PMID: 25603178
Green fluorescent protein with anionic tryptophan-based chromophore and long fluorescence lifetime.
Sarkisyan KS, Goryashchenko AS, Lidsky PV, Gorbachev DA, Bozhanova NG, Gorokhovatsky AY, Pereverzeva AR, Ryumina AP, Zherdeva VV, Savitsky AP, Solntsev KM, Bommarius AS, Sharonov GV, Lindquist JR, Drobizhev M, Hughes TE, Rebane A, Lukyanov KA, Mishin AS., Biophys J 109(2), 2015
PMID: 26200874
Photophysical processes in single molecule organic fluorescent probes.
Stennett EM, Ciuba MA, Levitus M., Chem Soc Rev 43(4), 2014
PMID: 24141280
Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry.
Gohar AV, Cao R, Jenkins P, Li W, Houston JP, Houston KD., Biomed Opt Express 4(8), 2013
PMID: 24010001
H2A.Z acidic patch couples chromatin dynamics to regulation of gene expression programs during ESC differentiation.
Subramanian V, Mazumder A, Surface LE, Butty VL, Fields PA, Alwan A, Torrey L, Thai KK, Levine SS, Bathe M, Boyer LA., PLoS Genet 9(8), 2013
PMID: 23990805
Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells.
Müller SM, Galliardt H, Schneider J, Barisas BG, Seidel T., Front Plant Sci 4(), 2013
PMID: 24194740
Neurotrophin signaling via long-distance axonal transport.
Chowdary PD, Che DL, Cui B., Annu Rev Phys Chem 63(), 2012
PMID: 22404590
Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis.
Calvo-Álvarez E, Guerrero NA, Alvarez-Velilla R, Prada CF, Requena JM, Punzón C, Llamas MÁ, Arévalo FJ, Rivas L, Fresno M, Pérez-Pertejo Y, Balaña-Fouce R, Reguera RM., PLoS Negl Trop Dis 6(11), 2012
PMID: 23209866
The cellular energization state affects peripheral stalk stability of plant vacuolar H+-ATPase and impairs vacuolar acidification.
Schnitzer D, Seidel T, Sander T, Golldack D, Dietz KJ., Plant Cell Physiol 52(5), 2011
PMID: 21474463
Fluorescence lifetime measurements and biological imaging.
Berezin MY, Achilefu S., Chem Rev 110(5), 2010
PMID: 20356094
Fluorescent proteins as light-inducible photochemical partners.
Lukyanov KA, Serebrovskaya EO, Lukyanov S, Chudakov DM., Photochem Photobiol Sci 9(10), 2010
PMID: 20672171
Multimodality imaging of gene transfer with a receptor-based reporter gene.
Chen R, Parry JJ, Akers WJ, Berezin MY, El Naqa IM, Achilefu S, Edwards WB, Rogers BE., J Nucl Med 51(9), 2010
PMID: 20720053
Single-molecule spectroscopy of fluorescent proteins.
Blum C, Subramaniam V., Anal Bioanal Chem 393(2), 2009
PMID: 18854990
Redox-dependent regulation of the stress-induced zinc-finger protein SAP12 in Arabidopsis thaliana.
Ströher E, Wang XJ, Roloff N, Klein P, Husemann A, Dietz KJ., Mol Plant 2(2), 2009
PMID: 19825620
Multiple redox and non-redox interactions define 2-Cys peroxiredoxin as a regulatory hub in the chloroplast.
Muthuramalingam M, Seidel T, Laxa M, Nunes de Miranda SM, Gärtner F, Ströher E, Kandlbinder A, Dietz KJ., Mol Plant 2(6), 2009
PMID: 19995730

34 References

Daten bereitgestellt von Europe PubMed Central.

The green fluorescent protein.
Tsien RY., Annu. Rev. Biochem. 67(), 1998
PMID: 9759496
The fluorescent toolbox for assessing protein location and function.
Giepmans BN, Adams SR, Ellisman MH, Tsien RY., Science 312(5771), 2006
PMID: 16614209
Fluorescent proteins as a toolkit for in vivo imaging.
Chudakov DM, Lukyanov S, Lukyanov KA., Trends Biotechnol. 23(12), 2005
PMID: 16269193
GFP-based FRET analysis in live cells.
Takanishi CL, Bykova EA, Cheng W, Zheng J., Brain Res. 1091(1), 2006
PMID: 16529720
Using intrinsically fluorescent proteins for plant cell imaging.
Dixit R, Cyr R, Gilroy S., Plant J. 45(4), 2006
PMID: 16441351
On/off blinking and switching behaviour of single molecules of green fluorescent protein.
Dickson RM, Cubitt AB, Tsien RY, Moerner WE., Nature 388(6640), 1997
PMID: 9237752
Improved fluorescent proteins for single-molecule research in molecular tracking and co-localization.
Steinmeyer R, Noskov A, Krasel C, Weber I, Dees C, Harms GS., J Fluoresc 15(5), 2005
PMID: 16341788
Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa.
Habuchi S, Ando R, Dedecker P, Verheijen W, Mizuno H, Miyawaki A, Hofkens J., Proc. Natl. Acad. Sci. U.S.A. 102(27), 2005
PMID: 15972810
Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein.
Habuchi S, Cotlet M, Gronheid R, Dirix G, Michiels J, Vanderleyden J, De Schryver FC, Hofkens J., J. Am. Chem. Soc. 125(28), 2003
PMID: 12848545
Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa.
Dedecker P, Hotta J, Flors C, Sliwa M, Uji-i H, Roeffaers MB, Ando R, Mizuno H, Miyawaki A, Hofkens J., J. Am. Chem. Soc. 129(51), 2007
PMID: 18047340

Neuweiler, Anal. Chem. 77(), 2005

Tinnefeld, Angew. Chem. Int. Ed. Engl. 44(), 0

Weiss, Science 283(), 2005
Single-molecule microscopy of the green fluorescent protein using simultaneous two-color excitation.
Jung G, Wiehler J, Steipe B, Brauchle C, Zumbusch A., Chemphyschem 2(6), 2001
PMID: 23686962
Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules.
Kusumi A, Nakada C, Ritchie K, Murase K, Suzuki K, Murakoshi H, Kasai RS, Kondo J, Fujiwara T., Annu Rev Biophys Biomol Struct 34(), 2005
PMID: 15869394

Moerner, J. Chem. Phys. 117(), 2002
Single-molecule imaging analysis of Ras activation in living cells.
Murakoshi H, Iino R, Kobayashi T, Fujiwara T, Ohshima C, Yoshimura A, Kusumi A., Proc. Natl. Acad. Sci. U.S.A. 101(19), 2004
PMID: 15123831

Zumbusch, Single Mol. 1(), 2000
Fluorescent proteins from nonbioluminescent Anthozoa species.
Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA., Nat. Biotechnol. 17(10), 1999
PMID: 10504696
Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting.
Ando R, Mizuno H, Miyawaki A., Science 306(5700), 2004
PMID: 15550670
New tools for in vivo fluorescence tagging.
Chapman S, Oparka KJ, Roberts AG., Curr. Opin. Plant Biol. 8(6), 2005
PMID: 16188488
Kindling fluorescent proteins for precise in vivo photolabeling.
Chudakov DM, Belousov VV, Zaraisky AG, Novoselov VV, Staroverov DB, Zorov DB, Lukyanov S, Lukyanov KA., Nat. Biotechnol. 21(2), 2003
PMID: 12524551
Photoswitchable cyan fluorescent protein for protein tracking.
Chudakov DM, Verkhusha VV, Staroverov DB, Souslova EA, Lukyanov S, Lukyanov KA., Nat. Biotechnol. 22(11), 2004
PMID: 15502815
A photoactivatable GFP for selective photolabeling of proteins and cells.
Patterson GH, Lippincott-Schwartz J., Science 297(5588), 2002
PMID: 12228718
Far-field optical nanoscopy.
Hell SW., Science 316(5828), 2007
PMID: 17525330
Imaging intracellular fluorescent proteins at nanometer resolution.
Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF., Science 313(5793), 2006
PMID: 16902090
Multicolor super-resolution imaging with photo-switchable fluorescent probes.
Bates M, Huang B, Dempsey GT, Zhuang X., Science 317(5845), 2007
PMID: 17702910
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY., Nat. Biotechnol. 22(12), 2004
PMID: 15558047
Carbocyanine dyes as efficient reversible single-molecule optical switch.
Heilemann M, Margeat E, Kasper R, Sauer M, Tinnefeld P., J. Am. Chem. Soc. 127(11), 2005
PMID: 15771514
Identification of different emitting species in the red fluorescent protein DsRed by means of ensemble and single-molecule spectroscopy.
Cotlet M, Hofkens J, Habuchi S, Dirix G, Van Guyse M, Michiels J, Vanderleyden J, De Schryver FC., Proc. Natl. Acad. Sci. U.S.A. 98(25), 2001
PMID: 11724946

Cotlet, J. Phys. Chem. B 105(), 2001

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