Fluorescent proteins for single-molecule fluorescence applications

Seefeldt B, Kasper R, Seidel T, Tinnefeld P, Dietz K-J, Heilemann M, Sauer M (2008)
Journal of Biophotonics 1(1): 74-82.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single-molecule applications, demonstrated by high photostability and rare fluorescence-intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single-molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications. [GRAPHICS] mCherry exhibits a relatively high photostability and brightness under single-molecule fluorescence imaging conditions. (C) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Stichworte
single-molecule fluorescence; fluorescent proteins; fluorescence microscopy; Fluorescence
Erscheinungsjahr
2008
Zeitschriftentitel
Journal of Biophotonics
Band
1
Ausgabe
1
Seite(n)
74-82
ISSN
1864-063X
eISSN
1864-0648
Page URI
https://pub.uni-bielefeld.de/record/1636544

Zitieren

Seefeldt B, Kasper R, Seidel T, et al. Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics. 2008;1(1):74-82.
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K. - J., Heilemann, M., & Sauer, M. (2008). Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics, 1(1), 74-82. doi:10.1002/jbio.200710024
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K. - J., Heilemann, M., and Sauer, M. (2008). Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics 1, 74-82.
Seefeldt, B., et al., 2008. Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics, 1(1), p 74-82.
B. Seefeldt, et al., “Fluorescent proteins for single-molecule fluorescence applications”, Journal of Biophotonics, vol. 1, 2008, pp. 74-82.
Seefeldt, B., Kasper, R., Seidel, T., Tinnefeld, P., Dietz, K.-J., Heilemann, M., Sauer, M.: Fluorescent proteins for single-molecule fluorescence applications. Journal of Biophotonics. 1, 74-82 (2008).
Seefeldt, Britta, Kasper, Robert, Seidel, Thorsten, Tinnefeld, Philip, Dietz, Karl-Josef, Heilemann, Mike, and Sauer, Markus. “Fluorescent proteins for single-molecule fluorescence applications”. Journal of Biophotonics 1.1 (2008): 74-82.

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PMID: 19343637
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