Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy

Grasser M, Kane CM, Merkle T, Melzer M, Emmersen J, Grasser KD (2009)
Journal of Molecular Biology 386(3): 598-611.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Grasser, Marion; Kane, Caroline M.; Merkle, ThomasUniBi; Melzer, Michael; Emmersen, Jeppe; Grasser, Klaus D.
Abstract / Bemerkung
Transcript elongation factor TFIIS promotes efficient transcription by RNA polymerase II, since it assists in bypassing blocks during mRNA synthesis. While yeast cells lacking TFIIS are viable, inactivation of mouse TFIIS causes embryonic lethality. Here, we have identified a protein encoded in the Arabidopsis genome that displays a marked sequence similarity to TFIIS of other organisms, primarily within domains II and III in the C-terminal part of the protein. TFIIS is widely expressed in Arabidopsis, and a green fluorescent protein-TFIIS fusion protein localises specifically to the cell nucleus. When expressed in yeast cells lacking the endogenous TFIIS, Arabidopsis TFIIS partially complements the sensitivity of mutant cells to the nucleotide analog 6-azauridine, which is a typical characteristic of transcript elongation factors. We have characterised Arabidopsis lines harbouring T-DNA insertions in the coding sequence of TFIIS. Plants homozygous for T-DNA insertions are viable, and genomewide transcript profiling revealed that compared to control plants, a relatively small number of genes are differentially expressed in mutant plants. TFIIS-/- plants display essentially normal development, but they flower slightly earlier than control plants and show clearly reduced seed dormancy. Plants with RNAi-mediated knockdown of TFIIS expression also are affected in seed dormancy. Therefore, TFIIS plays a critical role in Arabidopsis seed development. (c) 2008 Elsevier Ltd. All rights reserved.
RNA polymerase II; transcript elongation; transcriptome; yeast; nuclear localisation; complementation
Journal of Molecular Biology
Page URI


Grasser M, Kane CM, Merkle T, Melzer M, Emmersen J, Grasser KD. Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy. Journal of Molecular Biology. 2009;386(3):598-611.
Grasser, M., Kane, C. M., Merkle, T., Melzer, M., Emmersen, J., & Grasser, K. D. (2009). Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy. Journal of Molecular Biology, 386(3), 598-611.
Grasser, Marion, Kane, Caroline M., Merkle, Thomas, Melzer, Michael, Emmersen, Jeppe, and Grasser, Klaus D. 2009. “Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy”. Journal of Molecular Biology 386 (3): 598-611.
Grasser, M., Kane, C. M., Merkle, T., Melzer, M., Emmersen, J., and Grasser, K. D. (2009). Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy. Journal of Molecular Biology 386, 598-611.
Grasser, M., et al., 2009. Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy. Journal of Molecular Biology, 386(3), p 598-611.
M. Grasser, et al., “Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy”, Journal of Molecular Biology, vol. 386, 2009, pp. 598-611.
Grasser, M., Kane, C.M., Merkle, T., Melzer, M., Emmersen, J., Grasser, K.D.: Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy. Journal of Molecular Biology. 386, 598-611 (2009).
Grasser, Marion, Kane, Caroline M., Merkle, Thomas, Melzer, Michael, Emmersen, Jeppe, and Grasser, Klaus D. “Transcript Elongation Factor TFIIS Is Involved in Arabidopsis Seed Dormancy”. Journal of Molecular Biology 386.3 (2009): 598-611.

31 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

UVSSA, UBP12, and RDO2/TFIIS Contribute to Arabidopsis UV Tolerance.
Al Khateeb WM, Sher AA, Marcus JM, Schroeder DF., Front Plant Sci 10(), 2019
PMID: 31105721
Genome-metabolite associations revealed low heritability, high genetic complexity, and causal relations for leaf metabolites in winter wheat (Triticum aestivum).
Matros A, Liu G, Hartmann A, Jiang Y, Zhao Y, Wang H, Ebmeyer E, Korzun V, Schachschneider R, Kazman E, Schacht J, Longin F, Reif JC, Mock HP., J Exp Bot 68(3), 2017
PMID: 28007948
The Composition of the Arabidopsis RNA Polymerase II Transcript Elongation Complex Reveals the Interplay between Elongation and mRNA Processing Factors.
Antosz W, Pfab A, Ehrnsberger HF, Holzinger P, Köllen K, Mortensen SA, Bruckmann A, Schubert T, Längst G, Griesenbeck J, Schubert V, Grasser M, Grasser KD., Plant Cell 29(4), 2017
PMID: 28351991
The PHD-containing protein EARLY BOLTING IN SHORT DAYS regulates seed dormancy in Arabidopsis.
Narro-Diego L, López-González L, Jarillo JA, Piñeiro M., Plant Cell Environ 40(10), 2017
PMID: 28770581
Antisense transcription represses Arabidopsis seed dormancy QTL DOG1 to regulate drought tolerance.
Yatusevich R, Fedak H, Ciesielski A, Krzyczmonik K, Kulik A, Dobrowolska G, Swiezewski S., EMBO Rep 18(12), 2017
PMID: 29030481
Multifunctional RNA Binding Protein OsTudor-SN in Storage Protein mRNA Transport and Localization.
Chou HL, Tian L, Kumamaru T, Hamada S, Okita TW., Plant Physiol 175(4), 2017
PMID: 29084903
Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1.
Cyrek M, Fedak H, Ciesielski A, Guo Y, Sliwa A, Brzezniak L, Krzyczmonik K, Pietras Z, Kaczanowski S, Liu F, Swiezewski S., Plant Physiol 170(2), 2016
PMID: 26620523
Staying Alive: Molecular Aspects of Seed Longevity.
Sano N, Rajjou L, North HM, Debeaujon I, Marion-Poll A, Seo M., Plant Cell Physiol 57(4), 2016
PMID: 26637538
Control of seed dormancy in Arabidopsis by a cis-acting noncoding antisense transcript.
Fedak H, Palusinska M, Krzyczmonik K, Brzezniak L, Yatusevich R, Pietras Z, Kaczanowski S, Swiezewski S., Proc Natl Acad Sci U S A 113(48), 2016
PMID: 27856735
NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis.
Dolata J, Guo Y, Kołowerzo A, Smoliński D, Brzyżek G, Jarmołowski A, Świeżewski S., EMBO J 34(4), 2015
PMID: 25568310
Analysis of the transgenerational iron deficiency stress memory in Arabidopsis thaliana plants.
Murgia I, Giacometti S, Balestrazzi A, Paparella S, Pagliano C, Morandini P., Front Plant Sci 6(), 2015
PMID: 26442058
Synergistic exposure of rice seeds to different doses of γ-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway.
Macovei A, Garg B, Raikwar S, Balestrazzi A, Carbonera D, Buttafava A, Bremont JF, Gill SS, Tuteja N., Biomed Res Int 2014(), 2014
PMID: 24551849
The transcript elongation factor SPT4/SPT5 is involved in auxin-related gene expression in Arabidopsis.
Dürr J, Lolas IB, Sørensen BB, Schubert V, Houben A, Melzer M, Deutzmann R, Grasser M, Grasser KD., Nucleic Acids Res 42(7), 2014
PMID: 24497194
Functional consequences of splicing of the antisense transcript COOLAIR on FLC transcription.
Marquardt S, Raitskin O, Wu Z, Liu F, Sun Q, Dean C., Mol Cell 54(1), 2014
PMID: 24725596
Elongator and SPT4/SPT5 complexes as proxy to study RNA polymerase II transcript elongation control of plant development.
Van Lijsebettens M, Dürr J, Woloszynska M, Grasser KD., Proteomics 14(19), 2014
PMID: 24733746
TEF-7A, a transcript elongation factor gene, influences yield-related traits in bread wheat (Triticum aestivum L.).
Zheng J, Liu H, Wang Y, Wang L, Chang X, Jing R, Hao C, Zhang X., J Exp Bot 65(18), 2014
PMID: 25056774
Transcript elongation factors: shaping transcriptomes after transcript initiation.
Van Lijsebettens M, Grasser KD., Trends Plant Sci 19(11), 2014
PMID: 25131948
HISTONE DEACETYLASE 9 represses seedling traits in Arabidopsis thaliana dry seeds.
van Zanten M, Zöll C, Wang Z, Philipp C, Carles A, Li Y, Kornet NG, Liu Y, Soppe WJ., Plant J 80(3), 2014
PMID: 25146719
The conserved chimeric transcript UPGRADE2 is associated with unreduced pollen formation and is exclusively found in apomictic Boechera species.
Mau M, Corral JM, Vogel H, Melzer M, Fuchs J, Kuhlmann M, de Storme N, Geelen D, Sharbel TF., Plant Physiol 163(4), 2013
PMID: 24130193
S1 domain-containing STF modulates plastid transcription and chloroplast biogenesis in Nicotiana benthamiana.
Jeon Y, Jung HJ, Kang H, Park YI, Lee SH, Pai HS., New Phytol 193(2), 2012
PMID: 22050604
A novel role for histone methyltransferase KYP/SUVH4 in the control of Arabidopsis primary seed dormancy.
Zheng J, Chen F, Wang Z, Cao H, Li X, Deng X, Soppe WJ, Li Y, Liu Y., New Phytol 193(3), 2012
PMID: 22122546
TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress.
Gómez-Herreros F, de Miguel-Jiménez L, Morillo-Huesca M, Delgado-Ramos L, Muñoz-Centeno MC, Chávez S., Nucleic Acids Res 40(14), 2012
PMID: 22544605
Genotoxic stress and DNA repair in plants: emerging functions and tools for improving crop productivity.
Balestrazzi A, Confalonieri M, Macovei A, Donà M, Carbonera D., Plant Cell Rep 30(3), 2011
PMID: 21170715
Reduced expression of the DOG1 gene in Arabidopsis mutant seeds lacking the transcript elongation factor TFIIS.
Mortensen SA, Sønderkær M, Lynggaard C, Grasser M, Nielsen KL, Grasser KD., FEBS Lett 585(12), 2011
PMID: 21569772
Identification of the Arabidopsis REDUCED DORMANCY 2 gene uncovers a role for the polymerase associated factor 1 complex in seed dormancy.
Liu Y, Geyer R, van Zanten M, Carles A, Li Y, Hörold A, van Nocker S, Soppe WJ., PLoS One 6(7), 2011
PMID: 21799800
The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2.
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD., Plant J 61(4), 2010
PMID: 19947984
Cytokinin-binding protein (70 kDa) from etioplasts and amyloplasts of etiolated maize seedlings and chloroplasts of green plants and its putative function.
Brovko FA, Vasil'eva VS, Lushnikova AL, Selivankina SY, Karavaiko NN, Boziev KM, Shepelyakovskaya AO, Moshkov DA, Pavlik LL, Kusnetsov VV, Kulaeva ON., J Exp Bot 61(12), 2010
PMID: 20584787

70 References

Daten bereitgestellt von Europe PubMed Central.

Running with RNA polymerase: eukaryotic transcript elongation.
Arndt KM, Kane CM., Trends Genet. 19(10), 2003
PMID: 14550628
Breaking barriers to transcription elongation.
Saunders A, Core LJ, Lis JT., Nat. Rev. Mol. Cell Biol. 7(8), 2006
PMID: 16936696
Elongation by RNA polymerase II: the short and long of it.
Sims RJ 3rd, Belotserkovskaya R, Reinberg D., Genes Dev. 18(20), 2004
PMID: 15489290
Purification of a factor from Ehrlich ascites tumor cells specifically stimulating RNA polymerase II.
Sekimizu K, Kobayashi N, Mizuno D, Natori S., Biochemistry 15(23), 1976
PMID: 990265
Promoting elongation with transcript cleavage stimulatory factors.
Fish RN, Kane CM., Biochim. Biophys. Acta 1577(2), 2002
PMID: 12213659
Transcription elongation factor SII.
Wind M, Reines D., Bioessays 22(4), 2000
PMID: 10723030
RNA polymerase II structure: from core to functional complexes.
Cramer P., Curr. Opin. Genet. Dev. 14(2), 2004
PMID: 15196470
The RNA polymerase II elongation complex.
Shilatifard A, Conaway RC, Conaway JW., Annu. Rev. Biochem. 72(), 2003
PMID: 12676794
Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage.
Kettenberger H, Armache KJ, Cramer P., Cell 114(3), 2003
PMID: 12914699
Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS.
Kettenberger H, Armache KJ, Cramer P., Mol. Cell 16(6), 2004
PMID: 15610738
Efficient release from promoter-proximal stall sites requires transcript cleavage factor TFIIS.
Adelman K, Marr MT, Werner J, Saunders A, Ni Z, Andrulis ED, Lis JT., Mol. Cell 17(1), 2005
PMID: 15629721
TFIIS elongation factor and Mediator act in conjunction during transcription initiation in vivo.
Guglielmi B, Soutourina J, Esnault C, Werner M., Proc. Natl. Acad. Sci. U.S.A. 104(41), 2007
PMID: 17901206
The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes.
Kim B, Nesvizhskii AI, Rani PG, Hahn S, Aebersold R, Ranish JA., Proc. Natl. Acad. Sci. U.S.A. 104(41), 2007
PMID: 17913884
Genetic interaction between transcription elongation factor TFIIS and RNA polymerase II.
Archambault J, Lacroute F, Ruet A, Friesen JD., Mol. Cell. Biol. 12(9), 1992
PMID: 1508210
Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae.
Nakanishi T, Nakano A, Nomura K, Sekimizu K, Natori S., J. Biol. Chem. 267(19), 1992
PMID: 1618824
Transcription elongation factor S-II is required for definitive hematopoiesis.
Ito T, Arimitsu N, Takeuchi M, Kawamura N, Nagata M, Saso K, Akimitsu N, Hamamoto H, Natori S, Miyajima A, Sekimizu K., Mol. Cell. Biol. 26(8), 2006
PMID: 16581793
The Etched1 gene of Zea mays (L.) encodes a zinc ribbon protein that belongs to the transcriptionally active chromosome (TAC) of plastids and is similar to the transcription factor TFIIS.
da Costa e Silva O, Lorbiecke R, Garg P, Muller L, Wassmann M, Lauert P, Scanlon M, Hsia AP, Schnable PS, Krupinska K, Wienand U., Plant J. 38(6), 2004
PMID: 15165185
Genomic characterization of a testis-specific TFIIS (TCEA2) gene.
Weaver ZA, Kane CM., Genomics 46(3), 1997
PMID: 9441762
Chromosomal high mobility group (HMG) proteins of the HMGB-type occurring in the moss Physcomitrella patens.
Kiilerich B, Stemmer C, Merkle T, Launholt D, Gorr G, Grasser KD., Gene 407(1-2), 2007
PMID: 17980517
6-Azauracil inhibition of GTP biosynthesis in Saccharomyces cerevisiae.
Exinger F, Lacroute F., Curr. Genet. 22(1), 1992
PMID: 1611672


Genome-wide insertional mutagenesis of Arabidopsis thaliana.
Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR., Science 301(5633), 2003
PMID: 12893945
Arabidopsis HAF2 gene encoding TATA-binding protein (TBP)-associated factor TAF1, is required to integrate light signals to regulate gene expression and growth.
Bertrand C, Benhamed M, Li YF, Ayadi M, Lemonnier G, Renou JP, Delarue M, Zhou DX., J. Biol. Chem. 280(2), 2004
PMID: 15525647
Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis.
Ullah H, Chen JG, Young JC, Im KH, Sussman MR, Jones AM., Science 292(5524), 2001
PMID: 11408654
Emerging role for transcript elongation in plant development.
Grasser KD., Trends Plant Sci. 10(10), 2005
PMID: 16150628
Control of eukaryotic transcription elongation.
Winston F., Genome Biol. 2(2), 2001
PMID: 11182892
Contending with transcriptional arrest during RNAPII transcript elongation.
Svejstrup JQ., Trends Biochem. Sci. 32(4), 2007
PMID: 17349792
Knockdown of TFIIS by RNA silencing inhibits cancer cell proliferation and induces apoptosis.
Hubbard K, Catalano J, Puri RK, Gnatt A., BMC Cancer 8(), 2008
PMID: 18474089
Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex.
Davie JK, Kane CM., Mol. Cell. Biol. 20(16), 2000
PMID: 10913179
Genetic interactions between TFIIF and TFIIS.
Fish RN, Ammerman ML, Davie JK, Lu BF, Pham C, Howe L, Ponticelli AS, Kane CM., Genetics 173(4), 2006
PMID: 16648643
Seed Germination and Dormancy.
Bewley JD., Plant Cell 9(7), 1997
PMID: 12237375

Bradford, 2007
Seed dormancy and the control of germination.
Finch-Savage WE, Leubner-Metzger G., New Phytol. 171(3), 2006
PMID: 16866955
Seed dormancy and germination.
Koornneef M, Bentsink L, Hilhorst H., Curr. Opin. Plant Biol. 5(1), 2002
PMID: 11788305
Seed dormancy and germination
Bentsink, 2002
Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis.
Bentsink L, Jowett J, Hanhart CJ, Koornneef M., Proc. Natl. Acad. Sci. U.S.A. 103(45), 2006
PMID: 17065317
Cold and light control seed germination through the bHLH transcription factor SPATULA.
Penfield S, Josse EM, Kannangara R, Gilday AD, Halliday KJ, Graham IA., Curr. Biol. 15(22), 2005
PMID: 16303558
Differentially expressed genes associated with dormancy or germination of Arabidopsis thaliana seeds.
Toorop PE, Barroco RM, Engler G, Groot SP, Hilhorst HW., Planta 221(5), 2005
PMID: 15678336
The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth.
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inze D, Van Lijsebettens M., Plant Cell 19(2), 2007
PMID: 17329565
In vivo requirement of the RNA polymerase II elongation factor elongin A for proper gene expression and development.
Gerber M, Eissenberg JC, Kong S, Tenney K, Conaway JW, Conaway RC, Shilatifard A., Mol. Cell. Biol. 24(22), 2004
PMID: 15509793
A regulator of transcriptional elongation controls vertebrate neuronal development.
Guo S, Yamaguchi Y, Schilbach S, Wada T, Lee J, Goddard A, French D, Handa H, Rosenthal A., Nature 408(6810), 2000
PMID: 11099044
The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.
Kok FO, Oster E, Mentzer L, Hsieh JC, Henry CA, Sirotkin HI., Dev. Biol. 307(2), 2007
PMID: 17570355
The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth.
Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inze D, Van Lijsebettens M., Proc. Natl. Acad. Sci. U.S.A. 102(21), 2005
PMID: 15894610
Growth stage-based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants.
Boyes DC, Zayed AM, Ascenzi R, McCaskill AJ, Hoffman NE, Davis KR, Gorlach J., Plant Cell 13(7), 2001
PMID: 11449047
Linear models and empirical bayes methods for assessing differential expression in microarray experiments
Smyth, Stat. Appl. Genet. Mol. Biol. 3(), 2004
Controlling the false discovery rate: a practical and powerful approach to multiple testing
Benjamini, J. R. Stat. Soc. B 57(), 1995
Cytoscape: a software environment for integrated models of biomolecular interaction networks.
Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T., Genome Res. 13(11), 2003
PMID: 14597658

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 19150360
PubMed | Europe PMC

Suchen in

Google Scholar