Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete
Zeschnigk M, Martin M, Betzl G, Kalbe A, Sirsch C, Buiting K, Gross S, Fritzilas E, Frey B, Rahmann S, Horsthemke B (2009)
HUMAN MOLECULAR GENETICS 18(8): 1439-1448.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Zeschnigk, Michael;
Martin, MarcelUniBi;
Betzl, Gisela;
Kalbe, Andreas;
Sirsch, Caroline;
Buiting, Karin;
Gross, Stephanie;
Fritzilas, Epameinondas;
Frey, Bruno;
Rahmann, Sven;
Horsthemke, Bernhard
Einrichtung
Abstract / Bemerkung
Methylation of CpG islands (CGIs) plays an important role in gene silencing. For genome-wide methylation analysis of CGIs in female white blood cells and in sperm, we used four restriction enzymes and a size selection step to prepare DNA libraries enriched with CGIs. The DNA libraries were treated with sodium bisulfite and subjected to a modified 454/Roche Genome Sequencer protocol. We obtained 163 034 and 129 620 reads from blood and sperm, respectively, with an average read length of 133 bp. Bioinformatic analysis revealed that 12 358 (7.6%) blood library reads and 10 216 (7.9%) sperm library reads map to 6167 and 5796 different CGIs, respectively. In blood and sperm DNA, we identified 824 (13.7%) and 482 (8.5%) fully methylated autosomal CGIs, respectively. Differential methylation, which is characterized by the presence of methylated and unmethylated reads of the same CGI, was observed in 53 and 52 autosomal CGIs in blood and sperm DNA, respectively. Remarkably, methylation of X-chromosomal CGIs in female blood cells was most often incomplete (25-75%). Such incomplete methylation was mainly found on the X-chromosome, suggesting that it is linked to X-chromosome inactivation.
Erscheinungsjahr
2009
Zeitschriftentitel
HUMAN MOLECULAR GENETICS
Band
18
Ausgabe
8
Seite(n)
1439-1448
ISSN
0964-6906
eISSN
1460-2083
Page URI
https://pub.uni-bielefeld.de/record/1634615
Zitieren
Zeschnigk M, Martin M, Betzl G, et al. Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. HUMAN MOLECULAR GENETICS. 2009;18(8):1439-1448.
Zeschnigk, M., Martin, M., Betzl, G., Kalbe, A., Sirsch, C., Buiting, K., Gross, S., et al. (2009). Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. HUMAN MOLECULAR GENETICS, 18(8), 1439-1448. https://doi.org/10.1093/hmg/ddp054
Zeschnigk, Michael, Martin, Marcel, Betzl, Gisela, Kalbe, Andreas, Sirsch, Caroline, Buiting, Karin, Gross, Stephanie, et al. 2009. “Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete”. HUMAN MOLECULAR GENETICS 18 (8): 1439-1448.
Zeschnigk, M., Martin, M., Betzl, G., Kalbe, A., Sirsch, C., Buiting, K., Gross, S., Fritzilas, E., Frey, B., Rahmann, S., et al. (2009). Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. HUMAN MOLECULAR GENETICS 18, 1439-1448.
Zeschnigk, M., et al., 2009. Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. HUMAN MOLECULAR GENETICS, 18(8), p 1439-1448.
M. Zeschnigk, et al., “Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete”, HUMAN MOLECULAR GENETICS, vol. 18, 2009, pp. 1439-1448.
Zeschnigk, M., Martin, M., Betzl, G., Kalbe, A., Sirsch, C., Buiting, K., Gross, S., Fritzilas, E., Frey, B., Rahmann, S., Horsthemke, B.: Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. HUMAN MOLECULAR GENETICS. 18, 1439-1448 (2009).
Zeschnigk, Michael, Martin, Marcel, Betzl, Gisela, Kalbe, Andreas, Sirsch, Caroline, Buiting, Karin, Gross, Stephanie, Fritzilas, Epameinondas, Frey, Bruno, Rahmann, Sven, and Horsthemke, Bernhard. “Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete”. HUMAN MOLECULAR GENETICS 18.8 (2009): 1439-1448.
22 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
A hybrid parameter estimation algorithm for beta mixtures and applications to methylation state classification.
Schröder C, Rahmann S., Algorithms Mol Biol 12(), 2017
PMID: 28828033
Schröder C, Rahmann S., Algorithms Mol Biol 12(), 2017
PMID: 28828033
Analysis of DNA Methylation in Mammalian Cells.
Lizardi PM, Yan Q, Wajapeyee N., Cold Spring Harb Protoc 2017(12), 2017
PMID: 27852839
Lizardi PM, Yan Q, Wajapeyee N., Cold Spring Harb Protoc 2017(12), 2017
PMID: 27852839
A glimpse into past, present, and future DNA sequencing.
Morey M, Fernández-Marmiesse A, Castiñeiras D, Fraga JM, Couce ML, Cocho JA., Mol Genet Metab 110(1-2), 2013
PMID: 23742747
Morey M, Fernández-Marmiesse A, Castiñeiras D, Fraga JM, Couce ML, Cocho JA., Mol Genet Metab 110(1-2), 2013
PMID: 23742747
Deep bisulfite sequencing of aberrantly methylated loci in a patient with multiple methylation defects.
Beygo J, Ammerpohl O, Gritzan D, Heitmann M, Rademacher K, Richter J, Caliebe A, Siebert R, Horsthemke B, Buiting K., PLoS One 8(10), 2013
PMID: 24130816
Beygo J, Ammerpohl O, Gritzan D, Heitmann M, Rademacher K, Richter J, Caliebe A, Siebert R, Horsthemke B, Buiting K., PLoS One 8(10), 2013
PMID: 24130816
BSmooth: from whole genome bisulfite sequencing reads to differentially methylated regions.
Hansen KD, Langmead B, Irizarry RA., Genome Biol 13(10), 2012
PMID: 23034175
Hansen KD, Langmead B, Irizarry RA., Genome Biol 13(10), 2012
PMID: 23034175
Sequence overlap between autosomal and sex-linked probes on the Illumina HumanMethylation27 microarray.
Chen YA, Choufani S, Ferreira JC, Grafodatskaya D, Butcher DT, Weksberg R., Genomics 97(4), 2011
PMID: 21211562
Chen YA, Choufani S, Ferreira JC, Grafodatskaya D, Butcher DT, Weksberg R., Genomics 97(4), 2011
PMID: 21211562
Systematic cross-validation of 454 sequencing and pyrosequencing for the exact quantification of DNA methylation patterns with single CpG resolution.
Potapova A, Albat C, Hasemeier B, Haeussler K, Lamprecht S, Suerbaum S, Kreipe H, Lehmann U., BMC Biotechnol 11(), 2011
PMID: 21235780
Potapova A, Albat C, Hasemeier B, Haeussler K, Lamprecht S, Suerbaum S, Kreipe H, Lehmann U., BMC Biotechnol 11(), 2011
PMID: 21235780
Distinct interfacial biclique patterns between ssDNA-binding proteins and those with dsDNAs.
Zeng T, Li J, Liu J., Proteins 79(2), 2011
PMID: 21120860
Zeng T, Li J, Liu J., Proteins 79(2), 2011
PMID: 21120860
Correction of PCR-bias in quantitative DNA methylation studies by means of cubic polynomial regression.
Moskalev EA, Zavgorodnij MG, Majorova SP, Vorobjev IA, Jandaghi P, Bure IV, Hoheisel JD., Nucleic Acids Res 39(11), 2011
PMID: 21486748
Moskalev EA, Zavgorodnij MG, Majorova SP, Vorobjev IA, Jandaghi P, Bure IV, Hoheisel JD., Nucleic Acids Res 39(11), 2011
PMID: 21486748
Pipeline for large-scale microdroplet bisulfite PCR-based sequencing allows the tracking of hepitype evolution in tumors.
Herrmann A, Haake A, Ammerpohl O, Martin-Guerrero I, Szafranski K, Stemshorn K, Nothnagel M, Kotsopoulos SK, Richter J, Warner J, Olson J, Link DR, Schreiber S, Krawczak M, Platzer M, Nürnberg P, Siebert R, Hampe J., PLoS One 6(7), 2011
PMID: 21750708
Herrmann A, Haake A, Ammerpohl O, Martin-Guerrero I, Szafranski K, Stemshorn K, Nothnagel M, Kotsopoulos SK, Richter J, Warner J, Olson J, Link DR, Schreiber S, Krawczak M, Platzer M, Nürnberg P, Siebert R, Hampe J., PLoS One 6(7), 2011
PMID: 21750708
EFS shows biallelic methylation in uveal melanoma with poor prognosis as well as tissue-specific methylation.
Neumann LC, Weinhäusel A, Thomas S, Horsthemke B, Lohmann DR, Zeschnigk M., BMC Cancer 11(), 2011
PMID: 21871071
Neumann LC, Weinhäusel A, Thomas S, Horsthemke B, Lohmann DR, Zeschnigk M., BMC Cancer 11(), 2011
PMID: 21871071
Late-replicating heterochromatin is characterized by decreased cytosine methylation in the human genome.
Suzuki M, Oda M, Ramos MP, Pascual M, Lau K, Stasiek E, Agyiri F, Thompson RF, Glass JL, Jing Q, Sandstrom R, Fazzari MJ, Hansen RS, Stamatoyannopoulos JA, McLellan AS, Greally JM., Genome Res 21(11), 2011
PMID: 21957152
Suzuki M, Oda M, Ramos MP, Pascual M, Lau K, Stasiek E, Agyiri F, Thompson RF, Glass JL, Jing Q, Sandstrom R, Fazzari MJ, Hansen RS, Stamatoyannopoulos JA, McLellan AS, Greally JM., Genome Res 21(11), 2011
PMID: 21957152
Differential binding of Escherichia coli McrA protein to DNA sequences that contain the dinucleotide m5CpG.
Mulligan EA, Hatchwell E, McCorkle SR, Dunn JJ., Nucleic Acids Res 38(6), 2010
PMID: 20015968
Mulligan EA, Hatchwell E, McCorkle SR, Dunn JJ., Nucleic Acids Res 38(6), 2010
PMID: 20015968
BRAT: bisulfite-treated reads analysis tool.
Harris EY, Ponts N, Levchuk A, Roch KL, Lonardi S., Bioinformatics 26(4), 2010
PMID: 20031974
Harris EY, Ponts N, Levchuk A, Roch KL, Lonardi S., Bioinformatics 26(4), 2010
PMID: 20031974
Introduction into the analysis of high-throughput-sequencing based epigenome data.
Huss M., Brief Bioinform 11(5), 2010
PMID: 20457755
Huss M., Brief Bioinform 11(5), 2010
PMID: 20457755
The application of next generation sequencing in DNA methylation analysis.
Zhang Y, Jeltsch A., Genes (Basel) 1(1), 2010
PMID: 24710012
Zhang Y, Jeltsch A., Genes (Basel) 1(1), 2010
PMID: 24710012
Bisulfite sequencing of DNA.
Darst RP, Pardo CE, Ai L, Brown KD, Kladde MP., Curr Protoc Mol Biol Chapter 7(), 2010
PMID: 20583099
Darst RP, Pardo CE, Ai L, Brown KD, Kladde MP., Curr Protoc Mol Biol Chapter 7(), 2010
PMID: 20583099
Next generation sequencing: advances in characterizing the methylome.
Taylor KH, Shi H, Caldwell CW., Genes (Basel) 1(2), 2010
PMID: 24710039
Taylor KH, Shi H, Caldwell CW., Genes (Basel) 1(2), 2010
PMID: 24710039
Genome-wide DNA methylation maps in follicular lymphoma cells determined by methylation-enriched bisulfite sequencing.
Choi JH, Li Y, Guo J, Pei L, Rauch TA, Kramer RS, Macmil SL, Wiley GB, Bennett LB, Schnabel JL, Taylor KH, Kim S, Xu D, Sreekumar A, Pfeifer GP, Roe BA, Caldwell CW, Bhalla KN, Shi H., PLoS One 5(9), 2010
PMID: 20927367
Choi JH, Li Y, Guo J, Pei L, Rauch TA, Kramer RS, Macmil SL, Wiley GB, Bennett LB, Schnabel JL, Taylor KH, Kim S, Xu D, Sreekumar A, Pfeifer GP, Roe BA, Caldwell CW, Bhalla KN, Shi H., PLoS One 5(9), 2010
PMID: 20927367
Advances in genome-wide DNA methylation analysis.
Gupta R, Nagarajan A, Wajapeyee N., Biotechniques 49(4), 2010
PMID: 20964631
Gupta R, Nagarajan A, Wajapeyee N., Biotechniques 49(4), 2010
PMID: 20964631
Pash 3.0: A versatile software package for read mapping and integrative analysis of genomic and epigenomic variation using massively parallel DNA sequencing.
Coarfa C, Yu F, Miller CA, Chen Z, Harris RA, Milosavljevic A., BMC Bioinformatics 11(), 2010
PMID: 21092284
Coarfa C, Yu F, Miller CA, Chen Z, Harris RA, Milosavljevic A., BMC Bioinformatics 11(), 2010
PMID: 21092284
Inactive X chromosome-specific reduction in placental DNA methylation.
Cotton AM, Avila L, Penaherrera MS, Affleck JG, Robinson WP, Brown CJ., Hum Mol Genet 18(19), 2009
PMID: 19586922
Cotton AM, Avila L, Penaherrera MS, Affleck JG, Robinson WP, Brown CJ., Hum Mol Genet 18(19), 2009
PMID: 19586922
27 References
Daten bereitgestellt von Europe PubMed Central.
Unmethylated domains in vertebrate DNA.
Cooper DN, Taggart MH, Bird AP., Nucleic Acids Res. 11(3), 1983
PMID: 6188105
Cooper DN, Taggart MH, Bird AP., Nucleic Acids Res. 11(3), 1983
PMID: 6188105
A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNA.
Bird A, Taggart M, Frommer M, Miller OJ, Macleod D., Cell 40(1), 1985
PMID: 2981636
Bird A, Taggart M, Frommer M, Miller OJ, Macleod D., Cell 40(1), 1985
PMID: 2981636
Comprehensive analysis of CpG islands in human chromosomes 21 and 22.
Takai D, Jones PA., Proc. Natl. Acad. Sci. U.S.A. 99(6), 2002
PMID: 11891299
Takai D, Jones PA., Proc. Natl. Acad. Sci. U.S.A. 99(6), 2002
PMID: 11891299
Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation.
Deb-Rinker P, Ly D, Jezierski A, Sikorska M, Walker PR., J. Biol. Chem. 280(8), 2004
PMID: 15615706
Deb-Rinker P, Ly D, Jezierski A, Sikorska M, Walker PR., J. Biol. Chem. 280(8), 2004
PMID: 15615706
Generation of germline-competent induced pluripotent stem cells.
Okita K, Ichisaka T, Yamanaka S., Nature 448(7151), 2007
PMID: 17554338
Okita K, Ichisaka T, Yamanaka S., Nature 448(7151), 2007
PMID: 17554338
Restriction landmark genomic scanning method and its various applications.
Hayashizaki Y, Hirotsune S, Okazaki Y, Hatada I, Shibata H, Kawai J, Hirose K, Watanabe S, Fushiki S, Wada S., Electrophoresis 14(4), 1993
PMID: 8388788
Hayashizaki Y, Hirotsune S, Okazaki Y, Hatada I, Shibata H, Kawai J, Hirose K, Watanabe S, Fushiki S, Wada S., Electrophoresis 14(4), 1993
PMID: 8388788
Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells.
Weber M, Davies JJ, Wittig D, Oakeley EJ, Haase M, Lam WL, Schubeler D., Nat. Genet. 37(8), 2005
PMID: 16007088
Weber M, Davies JJ, Wittig D, Oakeley EJ, Haase M, Lam WL, Schubeler D., Nat. Genet. 37(8), 2005
PMID: 16007088
Microarray-based DNA methylation profiling: technology and applications.
Schumacher A, Kapranov P, Kaminsky Z, Flanagan J, Assadzadeh A, Yau P, Virtanen C, Winegarden N, Cheng J, Gingeras T, Petronis A., Nucleic Acids Res. 34(2), 2006
PMID: 16428248
Schumacher A, Kapranov P, Kaminsky Z, Flanagan J, Assadzadeh A, Yau P, Virtanen C, Winegarden N, Cheng J, Gingeras T, Petronis A., Nucleic Acids Res. 34(2), 2006
PMID: 16428248
Highly integrated single-base resolution maps of the epigenome in Arabidopsis.
Lister R, O'Malley RC, Tonti-Filippini J, Gregory BD, Berry CC, Millar AH, Ecker JR., Cell 133(3), 2008
PMID: 18423832
Lister R, O'Malley RC, Tonti-Filippini J, Gregory BD, Berry CC, Millar AH, Ecker JR., Cell 133(3), 2008
PMID: 18423832
Genome-scale DNA methylation maps of pluripotent and differentiated cells.
Meissner A, Mikkelsen TS, Gu H, Wernig M, Hanna J, Sivachenko A, Zhang X, Bernstein BE, Nusbaum C, Jaffe DB, Gnirke A, Jaenisch R, Lander ES., Nature 454(7205), 2008
PMID: 18600261
Meissner A, Mikkelsen TS, Gu H, Wernig M, Hanna J, Sivachenko A, Zhang X, Bernstein BE, Nusbaum C, Jaffe DB, Gnirke A, Jaenisch R, Lander ES., Nature 454(7205), 2008
PMID: 18600261
Genome sequencing in microfabricated high-density picolitre reactors.
Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM., Nature 437(7057), 2005
PMID: 16056220
Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM., Nature 437(7057), 2005
PMID: 16056220
Computational and experimental identification of novel human imprinted genes.
Luedi PP, Dietrich FS, Weidman JR, Bosko JM, Jirtle RL, Hartemink AJ., Genome Res. 17(12), 2007
PMID: 18055845
Luedi PP, Dietrich FS, Weidman JR, Bosko JM, Jirtle RL, Hartemink AJ., Genome Res. 17(12), 2007
PMID: 18055845
A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q.
Yamada Y, Watanabe H, Miura F, Soejima H, Uchiyama M, Iwasaka T, Mukai T, Sakaki Y, Ito T., Genome Res. 14(2), 2004
PMID: 14762061
Yamada Y, Watanabe H, Miura F, Soejima H, Uchiyama M, Iwasaka T, Mukai T, Sakaki Y, Ito T., Genome Res. 14(2), 2004
PMID: 14762061
Genome-wide profiling of DNA methylation reveals a class of normally methylated CpG island promoters.
Shen L, Kondo Y, Guo Y, Zhang J, Zhang L, Ahmed S, Shu J, Chen X, Waterland RA, Issa JP., PLoS Genet. 3(10), 2007
PMID: 17967063
Shen L, Kondo Y, Guo Y, Zhang J, Zhang L, Ahmed S, Shu J, Chen X, Waterland RA, Issa JP., PLoS Genet. 3(10), 2007
PMID: 17967063
A genome-wide screen for normally methylated human CpG islands that can identify novel imprinted genes.
Strichman-Almashanu LZ, Lee RS, Onyango PO, Perlman E, Flam F, Frieman MB, Feinberg AP., Genome Res. 12(4), 2002
PMID: 11932239
Strichman-Almashanu LZ, Lee RS, Onyango PO, Perlman E, Flam F, Frieman MB, Feinberg AP., Genome Res. 12(4), 2002
PMID: 11932239
Large-scale human promoter mapping using CpG islands.
Ioshikhes IP, Zhang MQ., Nat. Genet. 26(1), 2000
PMID: 10973249
Ioshikhes IP, Zhang MQ., Nat. Genet. 26(1), 2000
PMID: 10973249
Promoter CpG methylation contributes to ES cell gene regulation in parallel with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation.
Fouse SD, Shen Y, Pellegrini M, Cole S, Meissner A, Van Neste L, Jaenisch R, Fan G., Cell Stem Cell 2(2), 2008
PMID: 18371437
Fouse SD, Shen Y, Pellegrini M, Cole S, Meissner A, Van Neste L, Jaenisch R, Fan G., Cell Stem Cell 2(2), 2008
PMID: 18371437
X-inactivation profile reveals extensive variability in X-linked gene expression in females.
Carrel L, Willard HF., Nature 434(7031), 2005
PMID: 15772666
Carrel L, Willard HF., Nature 434(7031), 2005
PMID: 15772666
In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1.
Pfeifer GP, Tanguay RL, Steigerwald SD, Riggs AD., Genes Dev. 4(8), 1990
PMID: 2227409
Pfeifer GP, Tanguay RL, Steigerwald SD, Riggs AD., Genes Dev. 4(8), 1990
PMID: 2227409
WHO, 1999
High sensitivity mapping of methylated cytosines.
Clark SJ, Harrison J, Paul CL, Frommer M., Nucleic Acids Res. 22(15), 1994
PMID: 8065911
Clark SJ, Harrison J, Paul CL, Frommer M., Nucleic Acids Res. 22(15), 1994
PMID: 8065911
Imprinted segments in the human genome: different DNA methylation patterns in the Prader-Willi/Angelman syndrome region as determined by the genomic sequencing method.
Zeschnigk M, Schmitz B, Dittrich B, Buiting K, Horsthemke B, Doerfler W., Hum. Mol. Genet. 6(3), 1997
PMID: 9147641
Zeschnigk M, Schmitz B, Dittrich B, Buiting K, Horsthemke B, Doerfler W., Hum. Mol. Genet. 6(3), 1997
PMID: 9147641
MethPrimer: designing primers for methylation PCRs.
Li LC, Dahiya R., Bioinformatics 18(11), 2002
PMID: 12424112
Li LC, Dahiya R., Bioinformatics 18(11), 2002
PMID: 12424112
'Touchdown' PCR to circumvent spurious priming during gene amplification.
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS., Nucleic Acids Res. 19(14), 1991
PMID: 1861999
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS., Nucleic Acids Res. 19(14), 1991
PMID: 1861999
Somatic mosaicism in patients with Angelman syndrome and an imprinting defect.
Nazlican H, Zeschnigk M, Claussen U, Michel S, Boehringer S, Gillessen-Kaesbach G, Buiting K, Horsthemke B., Hum. Mol. Genet. 13(21), 2004
PMID: 15385437
Nazlican H, Zeschnigk M, Claussen U, Michel S, Boehringer S, Gillessen-Kaesbach G, Buiting K, Horsthemke B., Hum. Mol. Genet. 13(21), 2004
PMID: 15385437
CpG islands in vertebrate genomes.
Gardiner-Garden M, Frommer M., J. Mol. Biol. 196(2), 1987
PMID: 3656447
Gardiner-Garden M, Frommer M., J. Mol. Biol. 196(2), 1987
PMID: 3656447
Efficient q-gram filters for finding all epsilon-matches over a given length.
Rasmussen KR, Stoye J, Myers EW., J. Comput. Biol. 13(2), 2006
PMID: 16597241
Rasmussen KR, Stoye J, Myers EW., J. Comput. Biol. 13(2), 2006
PMID: 16597241
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 19223391
PubMed | Europe PMC
Suchen in
Google Scholar