PUB - Publikationen an der Universität Bielefeld

Microarray analysis has become a popular and routine method in functional genomics. It is typical for such experiments to involve a small number of replicates, which causes Unreliable estimates of the sample variance. Microarrays have fostered the development of new statistical methods to analyze data resulting from experiments with small sample sizes. In this study, we tackle the problem of evaluating the performance of statistical tests for generating ranked gene lists from two-channel direct comparisons. We propose all evaluation method based oil a oligonucleotide microarray with a large number of replicate spots yielding a maximum) of 400 replicates per gene. We apply Spearman's rank correlation coefficient to ranked gene-lists generated by eight widely used microarray specific test statistics, which are applied to small random samples. We could show that variance stabilizing methods such as Cyber-T, SAM, and LIMMA can he beneficial for very small sample sizes and that SAM and the t-test provide stronger control of the type I error rate than the other methods. Specifically, we report that for four replicates all methods to very high correlation with our reference standard. (C) 2009 Elsevier B.V. All rights reserved.