Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium
Batista FRX, Moraes AM, Büntemeyer H, Noll T (2009)
BIOLOGICALS 37(2): 108-118.
Zeitschriftenaufsatz
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Abstract / Bemerkung
The recombinant G glycoprotein from the surface of the rabies virus (RVGP) is a promising candidate as a rabies vaccine component and also for diagnostic purposes. In this study, RVGP production by transfected Drosophila melanogaster S2 cells cultivated in a serum-free medium (Supplemented IPL-41 medium) was carried out. The effects of pH and pO(2) were evaluated in batch culture in parallel spinner flasks. The use of a pH equal to 6.3 and a pO(2) of 40% air saturation resulted in the highest RVGP content. These conditions were also used in fed-batch mode, yielding a RVGP content level of 98 g/10(7) cells. The main nutrients consumed were glucose, glutamine, asparagine, serine and proline and the major metabolites produced were alanine and ammonia, according to the metabolism studies performed. Since RVGP is a transmembrane protein, two different methods for protein recovery were assessed and compared. Detergent-based cell disruption showed to be more effective than mechanical disruption with glass beads for glycoprotein recovery. (C) 2008 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.
Stichworte
S2 cells;
Growth kinetics;
Aeration;
Fed-batch culture;
RVGP
Erscheinungsjahr
2009
Zeitschriftentitel
BIOLOGICALS
Band
37
Ausgabe
2
Seite(n)
108-118
ISSN
1045-1056
Page URI
https://pub.uni-bielefeld.de/record/1633988
Zitieren
Batista FRX, Moraes AM, Büntemeyer H, Noll T. Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium. BIOLOGICALS. 2009;37(2):108-118.
Batista, F. R. X., Moraes, A. M., Büntemeyer, H., & Noll, T. (2009). Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium. BIOLOGICALS, 37(2), 108-118. https://doi.org/10.1016/j.biologicals.2008.11.001
Batista, Fabiana R. X., Moraes, Angela M., Büntemeyer, Heino, and Noll, Thomas. 2009. “Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium”. BIOLOGICALS 37 (2): 108-118.
Batista, F. R. X., Moraes, A. M., Büntemeyer, H., and Noll, T. (2009). Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium. BIOLOGICALS 37, 108-118.
Batista, F.R.X., et al., 2009. Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium. BIOLOGICALS, 37(2), p 108-118.
F.R.X. Batista, et al., “Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium”, BIOLOGICALS, vol. 37, 2009, pp. 108-118.
Batista, F.R.X., Moraes, A.M., Büntemeyer, H., Noll, T.: Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium. BIOLOGICALS. 37, 108-118 (2009).
Batista, Fabiana R. X., Moraes, Angela M., Büntemeyer, Heino, and Noll, Thomas. “Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium”. BIOLOGICALS 37.2 (2009): 108-118.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
6 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
An effective and simplified DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris.
Picotto LD, Sguazza GH, Tizzano MA, Galosi CM, Cavalitto SF, Pecoraro MR., Protein Expr Purif 132(), 2017
PMID: 28189633
Picotto LD, Sguazza GH, Tizzano MA, Galosi CM, Cavalitto SF, Pecoraro MR., Protein Expr Purif 132(), 2017
PMID: 28189633
Culturing Drosophila melanogaster (S2) in a chemostat.
Vieira PB, da Costa BL, de Fatima Pires Augusto E, Tonso A., Biotechnol Lett 37(3), 2015
PMID: 25367803
Vieira PB, da Costa BL, de Fatima Pires Augusto E, Tonso A., Biotechnol Lett 37(3), 2015
PMID: 25367803
Effect of hypothermic temperatures on production of rabies virus glycoprotein by recombinant Drosophila melanogaster S2 cells cultured in suspension.
Rossi N, Silva BG, Astray R, Swiech K, Pereira CA, Suazo CA., J Biotechnol 161(3), 2012
PMID: 22820340
Rossi N, Silva BG, Astray R, Swiech K, Pereira CA, Suazo CA., J Biotechnol 161(3), 2012
PMID: 22820340
Behavior of wild-type and transfected S2 cells cultured in two different media.
Batista FR, Greco KN, Astray RM, Jorge SA, Augusto EF, Pereira CA, Mendonça RZ, Moraes AM., Appl Biochem Biotechnol 163(1), 2011
PMID: 21161610
Batista FR, Greco KN, Astray RM, Jorge SA, Augusto EF, Pereira CA, Mendonça RZ, Moraes AM., Appl Biochem Biotechnol 163(1), 2011
PMID: 21161610
Adaptation of the "Dynamic Method" for measuring the specific respiration rate in oxygen transfer systems through diffusion membrane.
Pamboukian MM, Pereira CA, Augusto Ede F, Tonso A., Biotechnol J 6(12), 2011
PMID: 21648091
Pamboukian MM, Pereira CA, Augusto Ede F, Tonso A., Biotechnol J 6(12), 2011
PMID: 21648091
Rabies virus glycoprotein expression in Drosophila S2 cells. I: design of expression/selection vectors, subpopulations selection and influence of sodium butyrate and culture medium on protein expression.
Lemos MA, Santos AS, Astray RM, Pereira CA, Jorge SA., J Biotechnol 143(2), 2009
PMID: 19615415
Lemos MA, Santos AS, Astray RM, Pereira CA, Jorge SA., J Biotechnol 143(2), 2009
PMID: 19615415
28 References
Daten bereitgestellt von Europe PubMed Central.
Comparative production of human interleukin-2 fused with green fluorescent protein in several recombinant expression systems
Cha, Biochem Eng J 24(), 2005
Cha, Biochem Eng J 24(), 2005
High level expression of hepatitis B virus surface antigen in stably transfected Drosophila Schneider-2 cells.
Deml L, Wolf H, Wagner R., J. Virol. Methods 79(2), 1999
PMID: 10381089
Deml L, Wolf H, Wagner R., J. Virol. Methods 79(2), 1999
PMID: 10381089
Observation and modeling of induction effect on human transferring production from stably transfected Drosophila S2 cell culture
Lim, Enz Microb Technol 39(), 2006
Lim, Enz Microb Technol 39(), 2006
Expression, purification, and functional characterization of the serine protease inhibitor neuroserpin expressed in Drosophila S2 cells.
Hill RM, Brennan SO, Birch NP., Protein Expr. Purif. 22(3), 2001
PMID: 11483002
Hill RM, Brennan SO, Birch NP., Protein Expr. Purif. 22(3), 2001
PMID: 11483002
Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor.
Valle MA, Kester MB, Burns AL, Marx SJ, Spiegel AM, Shiloach J., Cytotechnology 35(2), 2001
PMID: 19003289
Valle MA, Kester MB, Burns AL, Marx SJ, Spiegel AM, Shiloach J., Cytotechnology 35(2), 2001
PMID: 19003289
Expression of human plasminogen in Drosophila Schneider S2 cells.
Nilsen SL, Castellino FJ., Protein Expr. Purif. 16(1), 1999
PMID: 10336871
Nilsen SL, Castellino FJ., Protein Expr. Purif. 16(1), 1999
PMID: 10336871
The ascension of wildlife rabies: a cause for public health concern or intervention?
Rupprecht CE, Smith JS, Fekadu M, Childs JE., Emerging Infect. Dis. 1(4), 1995
PMID: 8903179
Rupprecht CE, Smith JS, Fekadu M, Childs JE., Emerging Infect. Dis. 1(4), 1995
PMID: 8903179
Production methods for rabies vaccine.
Perez O, Paolazzi CC., J. Ind. Microbiol. Biotechnol. 18(5), 1997
PMID: 9218362
Perez O, Paolazzi CC., J. Ind. Microbiol. Biotechnol. 18(5), 1997
PMID: 9218362
Evaluation of various serum and animal protein free media for the production of a veterinary rabies vaccine in BHK-21 cells.
Kallel H, Jouini A, Majoul S, Rourou S., J. Biotechnol. 95(3), 2002
PMID: 12007860
Kallel H, Jouini A, Majoul S, Rourou S., J. Biotechnol. 95(3), 2002
PMID: 12007860
Animal sera derivatives and substitutes used in the manufacture of pharmaceuticals: viral safety and regulatory aspects
Castle, Dev Biol Standard 99(), 1999
Castle, Dev Biol Standard 99(), 1999
Fedbatch culture and dynamic nutrient feeding
Wlaschin, 2006
Wlaschin, 2006
Mendonça RZ, Pereira CA, Moraes AM. Evaluation of concentrated milk whey as a supplement for Sf9 Spodoptera frugiperda cells in culture
Batista, Electron J Biotechnol 9(), 2006
Batista, Electron J Biotechnol 9(), 2006
Formulation of a protein-free medium based on IPL-41 for the sustained growth of Drosophila melanogaster S2 cells.
Batista FR, Pereira CA, Mendonca RZ, Moraes AM., Cytotechnology 57(1), 2008
PMID: 19003168
Batista FR, Pereira CA, Mendonca RZ, Moraes AM., Cytotechnology 57(1), 2008
PMID: 19003168
Rabies virus glycoprotein expression in Drosophila S2 cells. I. Functional recombinant protein in stable co-transfected cell line.
Yokomizo AY, Jorge SA, Astray RM, Fernandes I, Ribeiro OG, Horton DS, Tonso A, Tordo N, Pereira CA., Biotechnol J 2(1), 2007
PMID: 17225257
Yokomizo AY, Jorge SA, Astray RM, Fernandes I, Ribeiro OG, Horton DS, Tonso A, Tordo N, Pereira CA., Biotechnol J 2(1), 2007
PMID: 17225257
Analytical approach for the extraction of recombinant membrane viral glycoprotein from stably transfected Drosophila melanogaster cells.
Astray RM, Augusto E, Yokomizo AY, Pereira CA., Biotechnol J 3(1), 2008
PMID: 18064610
Astray RM, Augusto E, Yokomizo AY, Pereira CA., Biotechnol J 3(1), 2008
PMID: 18064610
Methods for off-line analysis of nutrients and products in mammalian cell culture
Büntemeyer, 2007
Büntemeyer, 2007
Characterization of growth and metabolism of Drosophila melanogaster cells transfected with the rabies-virus glycoprotein gene.
Swiech K, da Silva CS, Arantes MK, dos Santos AS, Astray RM, Pereira CA, Suazo CA., Biotechnol. Appl. Biochem. 49(Pt 1), 2008
PMID: 17570830
Swiech K, da Silva CS, Arantes MK, dos Santos AS, Astray RM, Pereira CA, Suazo CA., Biotechnol. Appl. Biochem. 49(Pt 1), 2008
PMID: 17570830
Echalier, 1997
Insights into the central metabolism of Spodoptera frugiperda (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5) insect cells by radiolabeling studies.
Benslimane C, Elias CB, Hawari J, Kamen A., Biotechnol. Prog. 21(1), 2005
PMID: 15903243
Benslimane C, Elias CB, Hawari J, Kamen A., Biotechnol. Prog. 21(1), 2005
PMID: 15903243
Growth, nutrient consumption, and end-product accumulation in Sf-9 and BTI-EAA insect cell cultures: insights into growth limitation and metabolism.
Bedard C, Tom R, Kamen A., Biotechnol. Prog. 9(6), 1993
PMID: 7764350
Bedard C, Tom R, Kamen A., Biotechnol. Prog. 9(6), 1993
PMID: 7764350
Pathways of glutamine metabolism in Spodoptera frugiperda (Sf9) insect cells: evidence for the presence of the nitrogen assimilation system, and a metabolic switch by 1H/15N NMR.
Drews M, Doverskog M, Ohman L, Chapman BE, Jacobsson U, Kuchel PW, Haggstrom L., J. Biotechnol. 78(1), 2000
PMID: 10702908
Drews M, Doverskog M, Ohman L, Chapman BE, Jacobsson U, Kuchel PW, Haggstrom L., J. Biotechnol. 78(1), 2000
PMID: 10702908
Bioprocess development for the production of a recombinant MUC1 fusion protein expressed by CHO-K1 cells in protein-free medium.
Link T, Backstrom M, Graham R, Essers R, Zorner K, Gatgens J, Burchell J, Taylor-Papadimitriou J, Hansson GC, Noll T., J. Biotechnol. 110(1), 2004
PMID: 15099905
Link T, Backstrom M, Graham R, Essers R, Zorner K, Gatgens J, Burchell J, Taylor-Papadimitriou J, Hansson GC, Noll T., J. Biotechnol. 110(1), 2004
PMID: 15099905
Bioreactor culture of recombinant Drosophila melanogaster S2 cells: characterization of metabolic features related to cell growth and production of the rabies virus glycoprotein.
Swiech K, Rossi N, Silva BG, Jorge SA, Astray RM, Suazo CA., Cytotechnology 57(1), 2008
PMID: 19003173
Swiech K, Rossi N, Silva BG, Jorge SA, Astray RM, Suazo CA., Cytotechnology 57(1), 2008
PMID: 19003173
An insight into insect cell metabolism through selective nutrient manipulation
Mendonça, Biotechnol Progr 72(), 1999
Mendonça, Biotechnol Progr 72(), 1999
Optimization of a feed medium for fed-batch culture of insect cells using a genetic algorithm.
Marteijn RC, Jurrius O, Dhont J, de Gooijer CD, Tramper J, Martens DE., Biotechnol. Bioeng. 81(3), 2003
PMID: 12474249
Marteijn RC, Jurrius O, Dhont J, de Gooijer CD, Tramper J, Martens DE., Biotechnol. Bioeng. 81(3), 2003
PMID: 12474249
Effect of hyperosmolarity on recombinant protein productivity in baculovirus expression system.
Olejnik A, Grajek W, Marecik R., J. Biotechnol. 102(3), 2003
PMID: 12730004
Olejnik A, Grajek W, Marecik R., J. Biotechnol. 102(3), 2003
PMID: 12730004
Effects of shifts up and down in osmotic pressure on production of tissue plasminogen activator by Chinese hamster ovary cells in suspension.
Takagi M, Moriyama T, Yoshida T., J. Biosci. Bioeng. 91(5), 2001
PMID: 16233031
Takagi M, Moriyama T, Yoshida T., J. Biosci. Bioeng. 91(5), 2001
PMID: 16233031
Growth of recombinant Drosophila melanogaster Schneider 2 cells producing rabies virus glycoprotein in bioreactor employing serum-free medium.
Galesi AL, Aguiar MA, Astray RM, Augusto EF, Moraes AM., Cytotechnology 57(1), 2008
PMID: 19003175
Galesi AL, Aguiar MA, Astray RM, Augusto EF, Moraes AM., Cytotechnology 57(1), 2008
PMID: 19003175
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