Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment
Frey H, Paskarbeit J, Anselmetti D (2009)
APPLIED PHYSICS LETTERS 94(24): 241116.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Einrichtung
Abstract / Bemerkung
For nanobiophysical applications, scanning near-field optical microscopy must combine high optical resolution and single fluorescent molecule sensitivity with the ability to operate in aqueous solution. These requirements can be achieved using the electric field enhancement at the tip of illuminated silicon probes for atomic force miscroscopy (AFM), whereby single ATTO-740 dye molecules could be imaged at an optical resolution down to 20 nm under ambient conditions as well as in aqueous solution. Two illumination modes have been tested: (a) bottom illumination in a total internal reflection microscopy setup and (b) direct top illumination, both with dedicated phase-sensitive single photon counting technology in dynamic AFM mode of operation.
Stichworte
dyes;
fluorescence;
image resolution;
nanobiotechnology;
near-field scanning;
atomic force microscopy;
optical microscopy;
biological techniques;
bio-optics;
silicon;
photon counting
Erscheinungsjahr
2009
Zeitschriftentitel
APPLIED PHYSICS LETTERS
Band
94
Ausgabe
24
Art.-Nr.
241116
ISSN
0003-6951
Page URI
https://pub.uni-bielefeld.de/record/1633324
Zitieren
Frey H, Paskarbeit J, Anselmetti D. Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment. APPLIED PHYSICS LETTERS. 2009;94(24): 241116.
Frey, H., Paskarbeit, J., & Anselmetti, D. (2009). Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment. APPLIED PHYSICS LETTERS, 94(24), 241116. https://doi.org/10.1063/1.3155190
Frey, Heinrich, Paskarbeit, Jan, and Anselmetti, Dario. 2009. “Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment”. APPLIED PHYSICS LETTERS 94 (24): 241116.
Frey, H., Paskarbeit, J., and Anselmetti, D. (2009). Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment. APPLIED PHYSICS LETTERS 94:241116.
Frey, H., Paskarbeit, J., & Anselmetti, D., 2009. Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment. APPLIED PHYSICS LETTERS, 94(24): 241116.
H. Frey, J. Paskarbeit, and D. Anselmetti, “Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment”, APPLIED PHYSICS LETTERS, vol. 94, 2009, : 241116.
Frey, H., Paskarbeit, J., Anselmetti, D.: Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment. APPLIED PHYSICS LETTERS. 94, : 241116 (2009).
Frey, Heinrich, Paskarbeit, Jan, and Anselmetti, Dario. “Tip-enhanced single molecule fluorescence near-field microscopy in aqueous environment”. APPLIED PHYSICS LETTERS 94.24 (2009): 241116.
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