Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum

Barranco-Medina S, Krell T, Finkemeier I, Sevilla F, Lazaro J-J, Dietz K-J (2007)
PLANT PHYSIOLOGY AND BIOCHEMISTRY 45(10-11): 729-739.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The pea peroxiredoxin homologue PsPrxII F of the Arabidopsis thaliana mitochondrial AtPrxII F was isolated as cDNA and genomic DNA, and characterized in respect to its biochemical and molecular properties. The deduced amino acid sequence contains an N-terminal targeting address for mitochondrial import. Mitochondrial location of PsPrxII F was confirmed by immunocytochemistry. The mature enzyme, without the transit peptide, has a molecular mass of 18.75 kDa, and, at positions 59 and 84, carries the two catalytic cysteinyl residues which are characteristic for this particular Prx subgroup. Activity of site-directed mutagenized C84S-variant lacking the so-called resolving Cys dropped to about 12% of WT Prx while C59S lost its peroxidatic activity completely. Likewise, WT PsPrxII F and C84S-variant but not C59S protected plasmid DNA against strand breakage in a mixed function oxidation assay. WT PrxII F and the variant proteins aggregated to high mass oligomers not yet described for type II Prx. Upon oxidation with hydrogen peroxide PsPrxII F focussed in a series of spots of distinct pI but similar molecular masses in two-dimensional gels indicating different oxidation states of the protein. Using this technique, partial oxidation was also detected in leaf extracts and isolated mitochondria. PsPrxII F mRNA and protein accumulated in cold and heavy metals treated pea plants suggesting a particular function under stress. (C) 2007 Elsevier Masson SAS. All rights reserved.
Stichworte
antioxidant defence; redox signalling; hydrogen peroxide; Pisum sativum (Pea); peroxiredoxin; mitochondria
Erscheinungsjahr
2007
Zeitschriftentitel
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Band
45
Ausgabe
10-11
Seite(n)
729-739
ISSN
0981-9428
Page URI
https://pub.uni-bielefeld.de/record/1631548

Zitieren

Barranco-Medina S, Krell T, Finkemeier I, Sevilla F, Lazaro J-J, Dietz K-J. Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY. 2007;45(10-11):729-739.
Barranco-Medina, S., Krell, T., Finkemeier, I., Sevilla, F., Lazaro, J. - J., & Dietz, K. - J. (2007). Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 45(10-11), 729-739. doi:10.1016/j.plaphy.2007.07.017
Barranco-Medina, S., Krell, T., Finkemeier, I., Sevilla, F., Lazaro, J. - J., and Dietz, K. - J. (2007). Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY 45, 729-739.
Barranco-Medina, S., et al., 2007. Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 45(10-11), p 729-739.
S. Barranco-Medina, et al., “Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum”, PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 45, 2007, pp. 729-739.
Barranco-Medina, S., Krell, T., Finkemeier, I., Sevilla, F., Lazaro, J.-J., Dietz, K.-J.: Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum. PLANT PHYSIOLOGY AND BIOCHEMISTRY. 45, 729-739 (2007).
Barranco-Medina, Sergio, Krell, Tino, Finkemeier, Iris, Sevilla, Francisca, Lazaro, Juan-Jose, and Dietz, Karl-Josef. “Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum”. PLANT PHYSIOLOGY AND BIOCHEMISTRY 45.10-11 (2007): 729-739.

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