A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES

Miksch G, Arnold W, LENTZSCH P, PRIEFER UB, Pühler A (1994)
MOLECULAR & GENERAL GENETICS 242(5): 539-550.

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DOI
Autor*in
Miksch, GerdUniBi; Arnold, WalterUniBi; LENTZSCH, P; PRIEFER, UB; Pühler, AlfredUniBi
Einrichtung
Fakultät für Biologie
Technische Fakultät
Abstract / Bemerkung
A 4.6 kb DNA region of the Rhizobium meliloti strain AK631 was found to contain seven open reading frames (ORFs), all oriented in the same direction. The putative gene products of four of these ORFs were highly homologous to UreA, UreB and UreC of Klebsiella aerogenes, Proteus mirabilis, Proteus vulgaris and Canavalia ensiformis. The overall organisation of the DNA region analysed was ORF1, ureA (ORF 2), ORF3, ureB (ORF4), ORF5, ORF6 and ureC (ORF7), indicating that the organisation of the urease structural genes in R. meliloti differs from that of other urease genes so far characterized. ORF1 was incomplete; only the 3' end of the coding region was present. The six complete ORFs coded for polypeptides of 11.1 (UreA), 8.9 (ORF3), 10.8 (UreB), 15.0 (ORF5), 13.8 (ORF6) and 60.7 kDa (UreC). No sequence homology to known polypeptides could be detected for the gene products of ORF1, ORF3, ORF5 and ORF6. Using a lacZ fusion and insertional mutagenesis it was shown that the seven ORFs identified were all located in the same transcription unit. For mutational analysis a resistance gene cassette was introduced into each of the complete ORFs resulting in apolar mutations. Mutations in ureA, ureB and ureC, but not in ORF3, ORF5 and ORF6, abolished urease activity in R. meliloti. The determination of hydrogen uptake in these R. meliloti mutants revealed that only ORF6 and ureB are necessary for hydrogen uptake.
Stichworte
UREASE ACTIVITY; RHIZOBIUM MELILOTI; UREASE GENES; MUTATIONAL ANALYSIS; HYDROGENASE ACTIVITY
Erscheinungsjahr
1994
Zeitschriftentitel
MOLECULAR & GENERAL GENETICS
Band
242
Ausgabe
5
Seite(n)
539-550
ISSN
0026-8925
eISSN
1617-4623
Page URI
https://pub.uni-bielefeld.de/record/1629475

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Miksch G, Arnold W, LENTZSCH P, PRIEFER UB, Pühler A. A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS. 1994;242(5):539-550.
Miksch, G., Arnold, W., LENTZSCH, P., PRIEFER, U. B., & Pühler, A. (1994). A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS, 242(5), 539-550. https://doi.org/10.1007/BF00285277
Miksch, Gerd, Arnold, Walter, LENTZSCH, P, PRIEFER, UB, and Pühler, Alfred. 1994. “A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES”. MOLECULAR & GENERAL GENETICS 242 (5): 539-550.
Miksch, G., Arnold, W., LENTZSCH, P., PRIEFER, U. B., and Pühler, A. (1994). A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS 242, 539-550.
Miksch, G., et al., 1994. A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS, 242(5), p 539-550.
G. Miksch, et al., “A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES”, MOLECULAR & GENERAL GENETICS, vol. 242, 1994, pp. 539-550.
Miksch, G., Arnold, W., LENTZSCH, P., PRIEFER, U.B., Pühler, A.: A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES. MOLECULAR & GENERAL GENETICS. 242, 539-550 (1994).
Miksch, Gerd, Arnold, Walter, LENTZSCH, P, PRIEFER, UB, and Pühler, Alfred. “A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL GENES FOR UREASE (UREA, UREB, UREC) INTERRUPTED BY OTHER OPEN READING FRAMES”. MOLECULAR & GENERAL GENETICS 242.5 (1994): 539-550.

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