The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY

Bertram-Drogatz PA, Quester I, Becker A, Pühler A (1998)
MOLECULAR AND GENERAL GENETICS 257(4): 433-441.

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Bertram-Drogatz, P. A.; Quester, I.; Becker, A.; Pühler, AlfredUniBi
Abstract / Bemerkung
Sinorhizobium meliloti (Rhizobium meliloti) is able to produce two different exopolysaccharides, succinoglycan and galactoglucan. Mutations in the mucR gene of S. meliloti result in the stimulation of galactoglucan synthesis, while the type of succinoglycan produced is modified. In culture supernatants of a mucR mutant, low-molecular-weight succinoglycan is present, whereas no high-molecular-weight succinoglycan could be detected. The biosynthesis of succinoglycan is directed by the products of the exo gene cluster. Two DNA fragments from this cluster, one located in front of the exoH gene and one in the intergenic region between the divergently transcribed genes exoX and exoY, were shown to represent effective binding sites for MucR. Whereas the latter binding site contains an inverted repeat motif, the former does not. However, the binding of MucR did not strongly modify the transcription of the exo genes involved. In the mucR mutant the expression levels of exoH-lacZ and exoX-lacZ transcriptional fusions were found to be increased 1.5- and 1.7-fold, respectively. On the other hand, the expression level of an exoY-lacZ transcriptional fusion was found to be 1.5-fold lower in the mucR mutant than in the wild-type background. Comparison of the DNA sequences of MucR-binding sites provides insight into the structural requirements for binding of MucR.
Stichworte
exopolysaccharide biosynthesis; Sinorhizobium meliloti; regulation of gene expression; meliloti; Rhizobium
Erscheinungsjahr
1998
Zeitschriftentitel
MOLECULAR AND GENERAL GENETICS
Band
257
Ausgabe
4
Seite(n)
433-441
ISSN
0026-8925
eISSN
1432-1874
Page URI
https://pub.uni-bielefeld.de/record/1626278

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Bertram-Drogatz PA, Quester I, Becker A, Pühler A. The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY. MOLECULAR AND GENERAL GENETICS. 1998;257(4):433-441.
Bertram-Drogatz, P. A., Quester, I., Becker, A., & Pühler, A. (1998). The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY. MOLECULAR AND GENERAL GENETICS, 257(4), 433-441. https://doi.org/10.1007/s004380050667
Bertram-Drogatz, P. A., Quester, I., Becker, A., and Pühler, Alfred. 1998. “The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY”. MOLECULAR AND GENERAL GENETICS 257 (4): 433-441.
Bertram-Drogatz, P. A., Quester, I., Becker, A., and Pühler, A. (1998). The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY. MOLECULAR AND GENERAL GENETICS 257, 433-441.
Bertram-Drogatz, P.A., et al., 1998. The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY. MOLECULAR AND GENERAL GENETICS, 257(4), p 433-441.
P.A. Bertram-Drogatz, et al., “The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY”, MOLECULAR AND GENERAL GENETICS, vol. 257, 1998, pp. 433-441.
Bertram-Drogatz, P.A., Quester, I., Becker, A., Pühler, A.: The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY. MOLECULAR AND GENERAL GENETICS. 257, 433-441 (1998).
Bertram-Drogatz, P. A., Quester, I., Becker, A., and Pühler, Alfred. “The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY”. MOLECULAR AND GENERAL GENETICS 257.4 (1998): 433-441.

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PMID: 30467359
Genetic control and regulatory mechanisms of succinoglycan and curdlan biosynthesis in genus Agrobacterium.
Wu D, Li A, Ma F, Yang J, Xie Y., Appl Microbiol Biotechnol 100(14), 2016
PMID: 27255488
The prokaryotic zinc-finger: structure, function and comparison with the eukaryotic counterpart.
Malgieri G, Palmieri M, Russo L, Fattorusso R, Pedone PV, Isernia C., FEBS J 282(23), 2015
PMID: 26365095
Diverse genetic regulon of the virulence-associated transcriptional regulator MucR in Brucella abortus 2308.
Caswell CC, Elhassanny AE, Planchin EE, Roux CM, Weeks-Gorospe JN, Ficht TA, Dunman PM, Roop RM., Infect Immun 81(4), 2013
PMID: 23319565
Absence of functional TolC protein causes increased stress response gene expression in Sinorhizobium meliloti.
Santos MR, Cosme AM, Becker JD, Medeiros JM, Mata MF, Moreira LM., BMC Microbiol 10(), 2010
PMID: 20573193
Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility.
Bahlawane C, McIntosh M, Krol E, Becker A., Mol Plant Microbe Interact 21(11), 2008
PMID: 18842098
Rhizobial exopolysaccharides: genetic control and symbiotic functions.
Skorupska A, Janczarek M, Marczak M, Mazur A, Król J., Microb Cell Fact 5(), 2006
PMID: 16483356
CbrA is a stationary-phase regulator of cell surface physiology and legume symbiosis in Sinorhizobium meliloti.
Gibson KE, Campbell GR, Lloret J, Walker GC., J Bacteriol 188(12), 2006
PMID: 16740957
Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions.
Becker A, Bergès H, Krol E, Bruand C, Rüberg S, Capela D, Lauber E, Meilhoc E, Ampe F, de Bruijn FJ, Fourment J, Francez-Charlot A, Kahn D, Küster H, Liebe C, Pühler A, Weidner S, Batut J., Mol Plant Microbe Interact 17(3), 2004
PMID: 15000396
MucR and mucS activate exp genes transcription and galactoglucan production in Sinorhizobium meliloti EFB1.
Lloret J, Martín M, Oruezabal RI, Bonilla I, Rivilla R., Mol Plant Microbe Interact 15(1), 2002
PMID: 11843303
MucR is necessary for galactoglucan production in Sinorhizobium meliloti EFB1.
Martín M, Lloret J, Sánchez-Contreras M, Bonilla I, Rivilla R., Mol Plant Microbe Interact 13(1), 2000
PMID: 10656595
The origin of prokaryotic C2H2 zinc finger regulators.
Bouhouche N, Syvanen M, Kado CI., Trends Microbiol 8(2), 2000
PMID: 10664601
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