Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern

Droge M, Pühler A, Selbitschka W (1998)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
The transfer of genetic information between distantly or even unrelated organisms during evolution had been inferred from nucleotide sequence comparisons. These studies provided circumstantial evidence that in rare cases genes had been laterally transmitted amongst organisms of the domains bacteria, archaea and eukarya. Laboratory-based studies confirmed that the gene pools of the various domains of organisms are linked. Amongst the bacterial gene exchange mechanisms transduction, transformation and conjugation, the latter was identified as the mechanism with potentially the broadest host range of transfer. Previously, the issue of horizontal gene transfer has become important in the context of biosafety. Gene transfer studies carried out under more natural conditions such as in model ecosystems or in the environment established that all gene transfer mechanisms worked under these conditions. Moreover, environmental hot-spots were identified where favourable conditions such as nutrient enrichment increased the probability of genetic exchange among bacteria. In particular, the phytosphere was shown to provide conducive conditions for conjugative gene exchange. Concern has been expressed that transfer of recombinant DNA (e.g. antibiotic resistance genes) from genetically modified organisms (GMOs) such as transgenic plants to phytosphere bacteria may occur and thus contribute to the undesirable spread of antibiotic resistance determinants. Studies which were performed to address this issue clearly showed that such a transfer occurs, if at all, at extremely low frequency. (C) 1998 Elsevier Science B.V. All rights reserved.
hot-spot of gene transfer; interdomain gene transfer; phytosphere; transgenic plants; antibiotic resistance determinants
Page URI


Droge M, Pühler A, Selbitschka W. Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern. JOURNAL OF BIOTECHNOLOGY. 1998;64(1):75-90.
Droge, M., Pühler, A., & Selbitschka, W. (1998). Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern. JOURNAL OF BIOTECHNOLOGY, 64(1), 75-90. https://doi.org/10.1016/S0168-1656(98)00105-9
Droge, M, Pühler, Alfred, and Selbitschka, Werner. 1998. “Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern”. JOURNAL OF BIOTECHNOLOGY 64 (1): 75-90.
Droge, M., Pühler, A., and Selbitschka, W. (1998). Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern. JOURNAL OF BIOTECHNOLOGY 64, 75-90.
Droge, M., Pühler, A., & Selbitschka, W., 1998. Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern. JOURNAL OF BIOTECHNOLOGY, 64(1), p 75-90.
M. Droge, A. Pühler, and W. Selbitschka, “Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern”, JOURNAL OF BIOTECHNOLOGY, vol. 64, 1998, pp. 75-90.
Droge, M., Pühler, A., Selbitschka, W.: Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern. JOURNAL OF BIOTECHNOLOGY. 64, 75-90 (1998).
Droge, M, Pühler, Alfred, and Selbitschka, Werner. “Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern”. JOURNAL OF BIOTECHNOLOGY 64.1 (1998): 75-90.

60 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications.
Whitford CM, Dymek S, Kerkhoff D, März C, Schmidt O, Edich M, Droste J, Pucker B, Rückert C, Kalinowski J., J Biol Eng 12(), 2018
PMID: 30123321
Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production.
Piva LC, Bentacur MO, Reis VCB, De Marco JL, Moraes LMP, Torres FAG., Bioengineered 8(5), 2017
PMID: 28399696
Detection of dietary DNA, protein, and glyphosate in meat, milk, and eggs.
Van Eenennaam AL, Young AE., J Anim Sci 95(7), 2017
PMID: 28727079
Molecular mechanisms underlying the emergence of bacterial pathogens: an ecological perspective.
Bartoli C, Roux F, Lamichhane JR., Mol Plant Pathol 17(2), 2016
PMID: 26062772
Mechanism of azithromycin inhibition of HSL synthesis in Pseudomonas aeruginosa.
Zeng J, Zhang N, Huang B, Cai R, Wu B, E S, Fang C, Chen C., Sci Rep 6(), 2016
PMID: 27075730
A methodology for markerless genetic modifications in Azotobacter vinelandii.
Eberhart LJ, Knutson CM, Barney BM., J Appl Microbiol 120(6), 2016
PMID: 26854474
A framework to gauge the epidemic potential of plant pathogens in environmental reservoirs: the example of kiwifruit canker.
Bartoli C, Lamichhane JR, Berge O, Guilbaud C, Varvaro L, Balestra GM, Vinatzer BA, Morris CE., Mol Plant Pathol 16(2), 2015
PMID: 24986268
Generation of influenza virus from avian cells infected by Salmonella carrying the viral genome.
Zhang X, Kong W, Wanda SY, Xin W, Alamuri P, Curtiss R., PLoS One 10(3), 2015
PMID: 25742162
Precise excision of plastid DNA by the large serine recombinase Bxb1.
Shao M, Kumar S, Thomson JG., Plant Biotechnol J 12(3), 2014
PMID: 24261912
Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts.
Kanda K, Ishida T, Hirota R, Ono S, Motomura K, Ikeda T, Kitamura K, Kuroda A., J Biotechnol 182-183(), 2014
PMID: 24786825
Horizontal gene transfer does not occur between sFat-1 transgenic pigs and nontransgenic pigs.
Tang MX, Zheng XM, Hou J, Qian LL, Jiang SW, Cui WT, Li K., Theriogenology 79(4), 2013
PMID: 23265929
Building-in biosafety for synthetic biology.
Wright O, Stan GB, Ellis T., Microbiology 159(pt 7), 2013
PMID: 23519158
Horizontal transfer of antibiotic resistance genes in a membrane bioreactor.
Yang D, Wang J, Qiu Z, Jin M, Shen Z, Chen Z, Wang X, Zhang B, Li JW., J Biotechnol 167(4), 2013
PMID: 23942379
Broad host range plasmids.
Jain A, Srivastava P., FEMS Microbiol Lett 348(2), 2013
PMID: 23980652
Extended-spectrum β-lactamase producing Escherichia coli in hospital wastewaters and sewage treatment plants in Queensland, Australia.
Gündoğdu A, Jennison AV, Smith HV, Stratton H, Katouli M., Can J Microbiol 59(11), 2013
PMID: 24206356
Direct ethanol production from cellulosic materials by the hypersaline-tolerant white-rot fungus Phlebia sp. MG-60.
Kamei I, Hirota Y, Mori T, Hirai H, Meguro S, Kondo R., Bioresour Technol 112(), 2012
PMID: 22425400
Molecular-based environmental risk assessment of three varieties of genetically engineered cows.
Xu J, Zhao J, Wang J, Zhao Y, Zhang L, Chu M, Li N., Transgenic Res 20(5), 2011
PMID: 21221780
Horizontal gene transfer of virulence determinants in selected bacterial foodborne pathogens.
Kelly BG, Vespermann A, Bolton DJ., Food Chem Toxicol 47(5), 2009
PMID: 18420327
Gene transfer events and their occurrence in selected environments.
Kelly BG, Vespermann A, Bolton DJ., Food Chem Toxicol 47(5), 2009
PMID: 18639605
An overview of general features of risk assessments of genetically modified crops
Craig Wendy, Tepfer Mark, Degrassi Giuliano, Ripandelli Decio., Euphytica 164(3), 2008
PMID: IND44121419
Cycling of extracellular DNA in the soil environment
Levy-Booth DJ, Campbell RG, Gulden RH, Hart MM, Powell JR, Klironomos JN, Pauls KP, Swanton CJ, Trevors JT, Dunfield KE., Soil Biol Biochem 39(12), 2007
PMID: IND43983641
Long-term field release of bioluminescent Sinorhizobium meliloti strains to assess the influence of a recA mutation on the strains' survival.
Selbitschka W, Keller M, Miethling-Graff R, Dresing U, Schwieger F, Krahn I, Homann I, Dammann-Kalinowski T, Pühler A, Tebbe CC., Microb Ecol 52(3), 2006
PMID: 16924432
Using the nucleotide substitution rate matrix to detect horizontal gene transfer.
Hamady M, Betterton MD, Knight R., BMC Bioinformatics 7(), 2006
PMID: 17067382
Antibiotic resistance markers in genetically modified plants: a risk to human health?
Gay PB, Gillespie SH., Lancet Infect Dis 5(10), 2005
PMID: 16183518
The relevance of gene transfer to the safety of food and feed derived from genetically modified (GM) plants.
van den Eede G, Aarts H, Buhk HJ, Corthier G, Flint HJ, Hammes W, Jacobsen B, Midtvedt T, van der Vossen J, von Wright A, Wackernagel W, Wilcks A., Food Chem Toxicol 42(7), 2004
PMID: 15123384
Detection of genetically modified organisms by electrochemiluminescence PCR method.
Liu J, Xing D, Shen X, Zhu D., Biosens Bioelectron 20(3), 2004
PMID: 15494222
Ancient horizontal gene transfer.
Brown JR., Nat Rev Genet 4(2), 2003
PMID: 12560809
Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection.
Mannelli I, Minunni M, Tombelli S, Mascini M., Biosens Bioelectron 18(2-3), 2003
PMID: 12485759
Identification and characterization of the conjugal transfer region of the pCg1 plasmid from naphthalene-degrading Pseudomonas putida Cg1.
Park W, Jeon CO, Hohnstock-Ashe AM, Winans SC, Zylstra GJ, Madsen EL., Appl Environ Microbiol 69(6), 2003
PMID: 12788725
Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant.
Tennstedt T, Szczepanowski R, Braun S, Pühler A, Schlüter A., FEMS Microbiol Ecol 45(3), 2003
PMID: 19719593
Genetically modified crops: environmental and human health concerns.
Azevedo JL, Araujo WL., Mutat Res 544(2-3), 2003
PMID: 14644324
Children and genetically engineered food: potentials and problems.
Perr HA., J Pediatr Gastroenterol Nutr 35(4), 2002
PMID: 12394371
Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates.
Disqué-Kochem C, Battermann A, Strätz M, Dreiseikelmann B., Microbiol Res 156(2), 2001
PMID: 11572456
Expression of bar in the plastid genome confers herbicide resistance.
Lutz KA, Knapp JE, Maliga P., Plant Physiol 125(4), 2001
PMID: 11299340
Transformation of an oral bacterium via chromosomal integration of free DNA in the presence of human saliva.
Mercer DK, Scott KP, Melville CM, Glover LA, Flint HJ., FEMS Microbiol Lett 200(2), 2001
PMID: 11425469
Naphthalene and donor cell density influence field conjugation of naphthalene catabolism plasmids.
Hohnstock AM, Stuart-Keil KG, Kull EE, Madsen EL., Appl Environ Microbiol 66(7), 2000
PMID: 10877811
A gene containment strategy based on a restriction-modification system.
Torres B, Jaenecke S, Timmis KN, García JL, Díaz E., Environ Microbiol 2(5), 2000
PMID: 11233163
Conserved and hybrid meta-cleavage operons from PAH-degrading Burkholderia RP007.
Laurie AD, Lloyd-Jones G., Biochem Biophys Res Commun 262(1), 1999
PMID: 10448110

69 References

Daten bereitgestellt von Europe PubMed Central.

Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H., Gene 19(3), 1982
PMID: 6295884
High frequency of conjugation versus plasmid segregation of RP1 in epiphytic Pseudomonas syringae populations.
Bjorklof K, Suoniemi A, Haahtela K, Romantschuk M., Microbiology (Reading, Engl.) 141 ( Pt 10)(), 1995
PMID: 7582033

The mob and oriT mobilization functions of a bacterial plasmid promote its transfer to plants
Buchanan-Wollaston, Nature 328(), 1987




Multiple antibiotic resistance in Rhizobium japonicum.
Cole MA, Elkan GH., Appl. Environ. Microbiol. 37(5), 1979
PMID: 485137
Gene transfer from bacteria to mammalian cells.
Courvalin P, Goussard S, Grillot-Courvalin C., C. R. Acad. Sci. III, Sci. Vie 318(12), 1995
PMID: 8745635
Identification and classification of bacterial plasmids.
Couturier M, Bex F, Bergquist PL, Maas WK., Microbiol. Rev. 52(3), 1988
PMID: 3054468
Polyphasic taxonomy of Rhizobia: emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb. nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov.
de, Int. J. Syst. Bacteriol. 44(), 1994

Selectable marker genes: safe for plants?
Flavell, Nature Biotechnol. 10(), 1992
Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli.
Froman BE, Tait RC, Gottlieb LD., Mol. Gen. Genet. 217(1), 1989
PMID: 2549364
An Agrobacterium transformation in the evolution of the genus Nicotiana
Furner, Nature 319(), 1986
Heterokaryotic transmission of senescence plasmid DNA in Neurospora
Griffiths, Curr. Genet. 17(), 1990
R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa.
Haas D, Holloway BW., Mol. Gen. Genet. 144(3), 1976
PMID: 818504
Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast.
Heinemann JA, Sprague GF Jr., Nature 340(6230), 1989
PMID: 2666856
Screening of soil bacteria for plasmids carrying antibiotic resistance.
Henschke RB, Schmidt FRJ., Biol. Fertil. Soils 9(3), 1990
PMID: IND90043967
Crystal structure of chaperone protein PapD reveals an immunoglobulin fold.
Holmgren A, Branden CI., Nature 342(6247), 1989
PMID: 2478891
R plasmids in Streptococcus agalactiae (group B).
Horodniceanu T, Bouanchaud DH, Bieth G, Chabbert YA., Antimicrob. Agents Chemother. 10(5), 1976
PMID: 795369
Possible horizontal transfer of Drosophila genes by the mite Proctolaelaps regalis.
Houck MA, Clark JB, Peterson KR, Kidwell MG., Science 253(5024), 1991
PMID: 1653453
Transkingdom transfer of the phosphoglucose isomerase gene.
Katz LA., J. Mol. Evol. 43(5), 1996
PMID: 8875859
Horizontal transfer of a mitochondrial plasmid.
Kempken F., Mol. Gen. Genet. 248(1), 1995
PMID: 7651331
Evidence for phage-mediated gene transfer among Pseudomonas aeruginosa strains on the phylloplane.
Kidambi SP, Ripp S, Miller RV., Appl. Environ. Microbiol. 60(2), 1994
PMID: 8135513
pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.
Kieser T, Hopwood DA, Wright HM, Thompson CJ., Mol. Gen. Genet. 185(2), 1982
PMID: 6283316
Phylogenetic relationships among prokaryotic and eukaryotic catalases.
Klotz MG, Klassen GR, Loewen PC., Mol. Biol. Evol. 14(9), 1997
PMID: 9287428
Genetic studies with plasmid RP1 in Erwinia chrysanthemi strains pathogenic on maize
Lacy, Phytopathology 68(), 1978
Erwinia amylovora mutants and in planta-derived transconjugants resistant to oxytetracycline
Lacy, Can. J. Microbiol. 6(), 1984
In situ transfer of an exogenously isolated plasmid between Pseudomonas spp. in sugar beet rhizosphere
Lilley, Microbiology 140(), 1994
Bacterial gene transfer by natural genetic transformation in the environment.
Lorenz MG, Wackernagel W., Microbiol. Rev. 58(3), 1994
PMID: 7968924
Intergeneric conjugation between Escherichia coli and Streptomyces species.
Mazodier P, Petter R, Thompson C., J. Bacteriol. 171(6), 1989
PMID: 2656662
On the track of natural transformation in soil.
Paget E, Simonet P., FEMS Microbiol. Ecol. 15(1/2), 1994
PMID: IND20440222
Conjugal Transfer of Megaplasmid 2 between Rhizobium meliloti Strains in Alfalfa Nodules.
Pretorius-Guth IM, Puhler A, Simon R., Appl. Environ. Microbiol. 56(8), 1990
PMID: 16348248
Aminoglycoside 3′-phosphotransferase II (APH(3′)II): review of its safety and use in the production of genetically engineered plants
Redenbaugh, Food Biotechnol. 8(), 1994
High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.
Schafer A, Kalinowski J, Simon R, Seep-Feldhaus AH, Puhler A., J. Bacteriol. 172(3), 1990
PMID: 2106514
A multicopy plasmid of the extremely thermophilic archaeon Sulfolobus effects its transfer to recipients by mating.
Schleper C, Holz I, Janekovic D, Murphy J, Zillig W., J. Bacteriol. 177(15), 1995
PMID: 7635827
Prevalence of npt II and Tn5 in kanamycin-resistant bacteria from different environments.
Smalla K, Van Overbeek LS, Pukall R, Van Elsas JD., FEMS Microbiol. Ecol. 13(1), 1993
PMID: IND20381976


Evolution by acquisition: the cases for horizontal gene transfers
Smith, TIBS 17(), 1992

The biology of natural transformation
Stewart, Ann. Rev. Microbiol. 40(), 1986

Plasmid transfer by conjugation from Escherichia coli to gram-positive bacteria
Trieu-Cuot, FEMS Microbiol. Lett. 48(), 1987
Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli.
Trieu-Cuot P, Carlier C, Courvalin P., J. Bacteriol. 170(9), 1988
PMID: 3137216
Conjugative Transfer of Chromosomal Genes between Fluorescent Pseudomonads in the Rhizosphere of Wheat.
Troxler J, Azelvandre P, Zala M, Defago G, Haas D., Appl. Environ. Microbiol. 63(1), 1997
PMID: 16535486
Occurrence of antibiotic resistance among bacilli in Brazilian soils and the possible involvement of resistance plasmids
van, Plant Soil 94(), 1986
Bacterial conjugation between pseudomonads in the rhizosphere of wheat
van, FEMS Microbiol. Ecol. 53(), 1988
Detection of plasmid RP4 transfer in soil and rhizosphere, and the occurrence of homology to RP4 in soil bacteria
van, Curr. Microbiol. 19(), 1989

Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum.
Williams DR, Young DI, Young M., J. Gen. Microbiol. 136(5), 1990
PMID: 2199603
Chronicles from the Agrobacterium-plant cell DNA transfer story
Zambryski, Annu. Rev. Plant Physiol. Plant Mol. Biol. 43(), 1992

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 9823660
PubMed | Europe PMC

Suchen in

Google Scholar