The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements

Selbitschka W, Zekri S, Schroder G, Pühler A, Toro N (1999)
FEMS MICROBIOLOGY LETTERS 172(1): 1-7.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1 and ISRm220-13-5 are 1481 and 1550 base pairs (bp) in size, respectively. ISRm102F34-1 is bordered by 15 bp imperfect terminal inverted repeat sequences (two mismatches), whereas the terminal inverted repeat of ISRm220-13-5 has a length of 16 bp (two mismatches). Both insertion sequence elements generate a 6-bp target duplication upon transposition. The putative transposase enzymes of ISRm102F34-1 and ISRm220-13-5 consist of 449 or 448 amino acid residues with predicted molecular weights of 50.7 or 51.3 kDa and theoretical isoelectric points of 10.8 or 11.1, respectively. ISRm102F34-1 is identical in 98.9% of its nucleotide sequence to an apparently inactive copy of an insertion sequence element, designated ISRm7, which flanks the left-end of the nodule formation efficiency (nfe) region of plasmid pRmeGR4b of S. meliloti strain GR4. ISRm102F34-1 and ISRm220-13-5 are closely related since they show an overall identity of 57.0% at the nucleotide sequence level and of 47.3% at the deduced amino acid level of their putative transposases. Both insertion sequence elements displayed significant similarity to the Xanthomonas campestris ISXc6 and its homolog IS1478a. Since none of these insertion sequence elements could be allocated to existing families of insertion sequence elements, a new family is proposed. Analysis of the distribution of ISRm102F32-1/ISRm7 in various local S. meliloti populations sampled from Medicago sativa, Medicago sphaerocarpa and Melilotus alba host plants at different locations in Spain revealed its presence in 35% of the isolates with a copy number ranging from 1 to 5. Furthermore, ISXm102F34-1/ISRm7 homologs were identified in other rhizobial species. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Stichworte
transposable elements; transposase; pRmeGR4b; non-pSym plasmid; copy number; Rhizobium meliloti
Erscheinungsjahr
1999
Zeitschriftentitel
FEMS MICROBIOLOGY LETTERS
Band
172
Ausgabe
1
Seite(n)
1-7
ISSN
0378-1097
Page URI
https://pub.uni-bielefeld.de/record/1623474

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Selbitschka W, Zekri S, Schroder G, Pühler A, Toro N. The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements. FEMS MICROBIOLOGY LETTERS. 1999;172(1):1-7.
Selbitschka, W., Zekri, S., Schroder, G., Pühler, A., & Toro, N. (1999). The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements. FEMS MICROBIOLOGY LETTERS, 172(1), 1-7. doi:10.1016/S0378-1097(99)00008-7
Selbitschka, W., Zekri, S., Schroder, G., Pühler, A., and Toro, N. (1999). The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements. FEMS MICROBIOLOGY LETTERS 172, 1-7.
Selbitschka, W., et al., 1999. The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements. FEMS MICROBIOLOGY LETTERS, 172(1), p 1-7.
W. Selbitschka, et al., “The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements”, FEMS MICROBIOLOGY LETTERS, vol. 172, 1999, pp. 1-7.
Selbitschka, W., Zekri, S., Schroder, G., Pühler, A., Toro, N.: The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements. FEMS MICROBIOLOGY LETTERS. 172, 1-7 (1999).
Selbitschka, Werner, Zekri, S, Schroder, G, Pühler, Alfred, and Toro, N. “The Sinorhizobium meliloti insertion sequence (IS) elements ISRm102F34-1/ISRm7 and ISRm220-13-5 belong to a new family of insertion sequence elements”. FEMS MICROBIOLOGY LETTERS 172.1 (1999): 1-7.

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