Subunit C of the vacuolar H+-ATPase of Hordeum vulgare

Tavakoli N, Eckerskorn C, Golldack D, Dietz K-J (1999)
FEBS LETTERS 456(1): 68-72.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The molecular cloning of the first subunit C of the plant vacuolar NC-ATPase is reported, Tonoplast vesicles were purified from barley leaves by sucrose gradient centrifugation, and the tonoplast polypeptides were separated by two-dimensional (2-D) gel electrophoresis, Using an anti-ATPase holoenzyme antibody, a polypeptide was recognized in the molecular mass range of 40 kDa with an isoelectric point of about 6.0, and tentatively identified as subunit C, The polypeptide spot was excised from about 50 2-D gels and subjected to endo Lys C proteolysis, Two proteolytic peptides were sequenced and the amino acid sequences were used to design degenerated oligonucleotides, followed by PCR amplification with cDNA template and screening elf a cDNA library synthesized from Hordeum vulgare poly A mRNA of epidermis strips. The full length clone of 1.5 kbp contains an open reading frame of 1062 bp encoding a polypeptide of 354 amino acids with a molecular mass of 39 982 Da and an isoelectric point of 6.04, Amino acid identity with sequences of SUC from animals and fungi is in the range of 36.7 to 38.5%, Expression of the cloned gene was demonstrated by Northern blotting and RT-PCR, (C) 1999 Federation of European Biochemical Societies.
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FEBS LETTERS
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456
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1
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68-72
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Tavakoli N, Eckerskorn C, Golldack D, Dietz K-J. Subunit C of the vacuolar H+-ATPase of Hordeum vulgare. FEBS LETTERS. 1999;456(1):68-72.
Tavakoli, N., Eckerskorn, C., Golldack, D., & Dietz, K. - J. (1999). Subunit C of the vacuolar H+-ATPase of Hordeum vulgare. FEBS LETTERS, 456(1), 68-72. doi:10.1016/S0014-5793(99)00937-0
Tavakoli, N., Eckerskorn, C., Golldack, D., and Dietz, K. - J. (1999). Subunit C of the vacuolar H+-ATPase of Hordeum vulgare. FEBS LETTERS 456, 68-72.
Tavakoli, N., et al., 1999. Subunit C of the vacuolar H+-ATPase of Hordeum vulgare. FEBS LETTERS, 456(1), p 68-72.
N. Tavakoli, et al., “Subunit C of the vacuolar H+-ATPase of Hordeum vulgare”, FEBS LETTERS, vol. 456, 1999, pp. 68-72.
Tavakoli, N., Eckerskorn, C., Golldack, D., Dietz, K.-J.: Subunit C of the vacuolar H+-ATPase of Hordeum vulgare. FEBS LETTERS. 456, 68-72 (1999).
Tavakoli, N, Eckerskorn, C, Golldack, Dortje, and Dietz, Karl-Josef. “Subunit C of the vacuolar H+-ATPase of Hordeum vulgare”. FEBS LETTERS 456.1 (1999): 68-72.

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Phenotypic subunit composition of the tobacco (Nicotiana tabacum L.) vacuolar-type H(+)-translocating ATPase.
Drobny M, Schnölzer M, Fiedler S, Lüttge U, Fischer-Schliebs E, Christian AL, Ratajczak R., Biochim Biophys Acta 1564(1), 2002
PMID: 12101019

27 References

Daten bereitgestellt von Europe PubMed Central.


Klink, Bot. Acta 104(), 1991

Taiz, Bot. Acta 104(), 1991

AUTHOR UNKNOWN, 0
Topography and subunit stoichiometry of the coated vesicle proton pump.
Arai H, Terres G, Pink S, Forgac M., J. Biol. Chem. 263(18), 1988
PMID: 2897963
Subunit E of the vacuolar H(+)-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis.
Dietz KJ, Rudloff S, Ageorges A, Eckerskorn C, Fischer K, Arbinger B., Plant J. 8(4), 1995
PMID: 7496398

AUTHOR UNKNOWN, 0
cDNA sequence and homologies of the "57-kDa" nucleotide-binding subunit of the vacuolar ATPase from Arabidopsis.
Manolson MF, Ouellette BF, Filion M, Poole RJ., J. Biol. Chem. 263(34), 1988
PMID: 2903860
Cloning of the V-ATPase subunit G in plant: functional expression and sub-cellular localization.
Rouquie D, Tournaire-Roux C, Szponarski W, Rossignol M, Doumas P., FEBS Lett. 437(3), 1998
PMID: 9824309

Zimniak, J. Biol. Chem. 263(), 1988

Brune, J. Exp. Bot. 45(), 1994
separation and Immunological Characterization of Membrane Fractions from Barley Roots.
Dupont FM, Tanaka CK, Hurkman WJ., Plant Physiol. 86(3), 1988
PMID: 16665976
High resolution two-dimensional electrophoresis of proteins.
O'Farrell PH., J. Biol. Chem. 250(10), 1975
PMID: 236308

Fairbanks, Biochemistry 4(), 1971

AUTHOR UNKNOWN, 0
A simple method for displaying the hydropathic character of a protein.
Kyte J, Doolittle RF., J. Mol. Biol. 157(1), 1982
PMID: 7108955
Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase.
van Hille B, Vanek M, Richener H, Green JR, Bilbe G., Biochem. Biophys. Res. Commun. 197(1), 1993
PMID: 8250920

Nelson, J. Biol. Chem. 265(), 1990
Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase.
Beltran C, Kopecky J, Pan YC, Nelson H, Nelson N., J. Biol. Chem. 267(2), 1992
PMID: 1730668

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