The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan

Moreira LM, Becker JD, Pühler A, Becker A (2000)
MICROBIOLOGY-UK 146: 2237-2248.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Moreira, Leonilde M.; Becker, Jörg D.; Pühler, AlfredUniBi ; Becker, Anke
Abstract / Bemerkung
In Sinorhizobium meliloti the biosynthesis of the exopolysaccharide galactoglucan (EPS II) is directed by the exp genes. the expD1 and expD2 gene products are homologous to components of type I secretion systems. ExpE1, the gene of which is located adjacent to expD1 and expD2, was defected in S. meliloti cells and culture supernatants. ExpD1 and ExpD2 were required for the secretion of ExpE1, indicating that ExpE1 is secreted by a type I secretion system involving ExpD1 and ExpD2. ExpE1 contains 15 aspartate- and glycine-rich nonapeptide repeats that were suggested to bind Ca2+. The ability to bind Ca2+ was demonstrated for a recombinant ExpE1 protein. Extracellular EPS II was not detected in cultures of non-polar expD1, expD2 and expE1 deletion mutants implying that these three genes are required for biosynthesis or secretion of galactoglucan.
Stichworte
exopolysaccharides; Rhizobium meliloti; type I secretion; protein; Ca2+-binding
Erscheinungsjahr
2000
Zeitschriftentitel
MICROBIOLOGY-UK
Band
146
Seite(n)
2237-2248
ISSN
1350-0872
Page URI
https://pub.uni-bielefeld.de/record/1619044

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Moreira LM, Becker JD, Pühler A, Becker A. The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan. MICROBIOLOGY-UK. 2000;146:2237-2248.
Moreira, L. M., Becker, J. D., Pühler, A., & Becker, A. (2000). The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan. MICROBIOLOGY-UK, 146, 2237-2248. https://doi.org/10.1099/00221287-146-9-2237
Moreira, Leonilde M., Becker, Jörg D., Pühler, Alfred, and Becker, Anke. 2000. “The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan”. MICROBIOLOGY-UK 146: 2237-2248.
Moreira, L. M., Becker, J. D., Pühler, A., and Becker, A. (2000). The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan. MICROBIOLOGY-UK 146, 2237-2248.
Moreira, L.M., et al., 2000. The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan. MICROBIOLOGY-UK, 146, p 2237-2248.
L.M. Moreira, et al., “The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan”, MICROBIOLOGY-UK, vol. 146, 2000, pp. 2237-2248.
Moreira, L.M., Becker, J.D., Pühler, A., Becker, A.: The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan. MICROBIOLOGY-UK. 146, 2237-2248 (2000).
Moreira, Leonilde M., Becker, Jörg D., Pühler, Alfred, and Becker, Anke. “The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan”. MICROBIOLOGY-UK 146 (2000): 2237-2248.

9 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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PMID: 22717884
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60 References

Daten bereitgestellt von Europe PubMed Central.


AUTHOR UNKNOWN, 0
Calcium-dependent conformation of E. coli alpha-haemolysin. Implications for the mechanism of membrane insertion and lysis.
Bakas L, Veiga MP, Soloaga A, Ostolaza H, Goni FM., Biochim. Biophys. Acta 1368(2), 1998
PMID: 9459600

AUTHOR UNKNOWN, 1998
The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products.
Becker A, Ruberg S, Kuster H, Roxlau AA, Keller M, Ivashina T, Cheng HP, Walker GC, Puhler A., J. Bacteriol. 179(4), 1997
PMID: 9023225
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098

AUTHOR UNKNOWN, 1984
Protein secretion by Gram-negative bacterial ABC exporters--a review.
Binet R, Letoffe S, Ghigo JM, Delepelaire P, Wandersman C., Gene 192(1), 1997
PMID: 9224868
Cyclic beta-glucans of members of the family Rhizobiaceae.
Breedveld MW, Miller KJ., Microbiol. Rev. 58(2), 1994
PMID: 8078434

AUTHOR UNKNOWN, Biotechniques 5(), 1987

AUTHOR UNKNOWN, J Bacteriol 113(), 1979

AUTHOR UNKNOWN, 0
ABC transporters: bacterial exporters.
Fath MJ, Kolter R., Microbiol. Rev. 57(4), 1993
PMID: 8302219
General transduction in Rhizobium meliloti.
Finan TM, Hartweig E, LeMieux K, Bergman K, Walker GC, Signer ER., J. Bacteriol. 159(1), 1984
PMID: 6330024
Cloning of an Azorhizobium caulinodans endoglucanase gene and analysis of its role in symbiosis.
Geelen D, van Montagu M, Holsters M., Appl. Environ. Microbiol. 61(9), 1995
PMID: 7574641

AUTHOR UNKNOWN, Proc Natl Acad Sci USA 93(), 1996

AUTHOR UNKNOWN, 0
SDS-PAGE strongly overestimates the molecular masses of the neurofilament proteins.
Kaufmann E, Geisler N, Weber K., FEBS Lett. 170(1), 1984
PMID: 6723964

AUTHOR UNKNOWN, Mol Plant-Microbe Interact 8(), 1995

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Exopolysaccharides of Rhizobium: synthesis, regulation and symbiotic function.
Leigh JA, Walker GC., Trends Genet. 10(2), 1994
PMID: 8191588
Secretion of haemolysin by Escherichia coli.
Mackman N, Nicaud JM, Gray L, Holland IB., Curr. Top. Microbiol. Immunol. 125(), 1986
PMID: 3017638
An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein.
Maina CV, Riggs PD, Grandea AG 3rd, Slatko BE, Moran LS, Tagliamonte JA, McReynolds LA, Guan CD., Gene 74(2), 1988
PMID: 3073105
Cell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii.
Mateos PF, Jimenez-Zurdo JI, Chen J, Squartini AS, Haack SK, Martinez-Molina E, Hubbell DH, Dazzo FB., Appl. Environ. Microbiol. 58(6), 1992
PMID: 1622257

AUTHOR UNKNOWN, 0
Culture medium for enterobacteria.
Neidhardt FC, Bloch PL, Smith DF., J. Bacteriol. 119(3), 1974
PMID: 4604283

AUTHOR UNKNOWN, FEMS Microbiol 27(), 1985
Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021.
Reinhold BB, Chan SY, Reuber TL, Marra A, Walker GC, Reinhold VN., J. Bacteriol. 176(7), 1994
PMID: 8144468

AUTHOR UNKNOWN, Microbiology 145(), 1999

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, Biotechnology 1(), 1983

AUTHOR UNKNOWN, Proc Natl Acad Sci USA 91(), 1994

AUTHOR UNKNOWN, Proc Natl Acad Sci USA 76(), 1979

AUTHOR UNKNOWN, Proc Natl Acad Sci USA 86(), 1989
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