Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism

Baier M, Noctor G, Foyer CH, Dietz K-J (2000)
PLANT PHYSIOLOGY 124(2): 823-832.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ;
Abstract / Bemerkung
The aim of this study was to characterize the effect of decreased 2-cysteine peroxiredoxin (2-CP) on the leaf anti-oxidative system in Arabidopsis. At three stages of leaf development, two lines of transgenic Arabidopsis mutants with decreased contents of chloroplast 2-CP were compared with wild ty-pe and a control line transformed with an empty vector. Glutathione contents and redox state were similar in all plants, and no changes in transcript levels for enzymes involved in glutathione metabolism were observed. Transcript levels for chloroplastic glutathione peroxidase were much lower than those for 2-CP, and both cytosolic and chloroplastic glutathione peroxidase were not increased in the mutants. In contrast, the foliar ascorbate pool was more oxidized in the mutants, although the difference decreased with plant age. The activities of thylakoid and stromal ascorbate peroxidase and particularly monodehydroascorbate reductase were increased as were transcripts for these enzymes. No change in dehydroascorbate reductase activity was observed, and effects on transcript abundance for glutathione reductase, catalase, and superoxide dismutase were slight or absent. The results demonstrate that 2-CP forms an integral part of the anti-oxidant network of chloroplasts and is functionally interconnected with other defense systems. Suppression of 2-CP leads to increased expression of other anti-oxidative genes possibly mediated by increased oxidation state of the leaf ascorbate pool.
Erscheinungsjahr
2000
Zeitschriftentitel
PLANT PHYSIOLOGY
Band
124
Ausgabe
2
Seite(n)
823-832
ISSN
0032-0889
eISSN
1532-2548
Page URI
https://pub.uni-bielefeld.de/record/1618785

Zitieren

Baier M, Noctor G, Foyer CH, Dietz K-J. Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism. PLANT PHYSIOLOGY. 2000;124(2):823-832.
Baier, M., Noctor, G., Foyer, C. H., & Dietz, K. - J. (2000). Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism. PLANT PHYSIOLOGY, 124(2), 823-832. doi:10.1104/pp.124.2.823
Baier, M., Noctor, G., Foyer, C. H., and Dietz, K. - J. (2000). Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism. PLANT PHYSIOLOGY 124, 823-832.
Baier, M., et al., 2000. Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism. PLANT PHYSIOLOGY, 124(2), p 823-832.
M. Baier, et al., “Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism”, PLANT PHYSIOLOGY, vol. 124, 2000, pp. 823-832.
Baier, M., Noctor, G., Foyer, C.H., Dietz, K.-J.: Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism. PLANT PHYSIOLOGY. 124, 823-832 (2000).
Baier, M, Noctor, G, Foyer, CH, and Dietz, Karl-Josef. “Antisense suppression of 2-cysteine peroxiredoxin in arabidopsis specifically enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione metabolism”. PLANT PHYSIOLOGY 124.2 (2000): 823-832.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 11027730
PubMed | Europe PMC

Suchen in

Google Scholar