The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules

Fruhling M, Schroder G, Hohnjec N, Pühler A, Perlick AM, Küster H (2000)
PLANT SCIENCE 160(1): 67-75.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
DOI
Autor*in
Fruhling, M; Schroder, G; Hohnjec, N; Pühler, AlfredUniBi ; Perlick, AM; Küster, Helge
Einrichtung
Fakultät für Biologie
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Abstract / Bemerkung
A full-length cDNA encoding the Vicia faba L. early nodulin VfEnod12 was isolated. The deduced protein sequence specified a 90 amino acid protein with a MW of 10 206 and contained a putative signal peptide sequence followed by PPX3 repeats characteristic of Enod12 proteins. The VfEnod12 gene was found to be expressed specifically in root nodules as early as 3 days post inoculation with Rhizobium leguminosarum bv. viciae. In mature nodules, VfEnod12 transcripts were confined to the prefixing zone II. A 3.3 kb genomic fragment carrying the complete VfEnod12 coding region was isolated. No intervening sequences were identified in the coding region. A promoter fragment carrying the -692/-41 region mediated reporter gene expression in root cortical cells, module primordia and the prefixing zone II of transgenic Vicia hirsuta root nodules. This fragment contained a putative binding site for the transcription factor ENBP1. In contrast to the highly conserved terminal AATAA motif of the ENBP1 binding site of known Enod12 promoters, the VfEnod12 promoter was characterized by an altered terminal AATAT sequence. This alteration did not interfere with VfEnod12 promoter activity in transgenic roots and nodules of V. hirsuta. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
Stichworte
promoter activity; hairy roots; ENBP1 binding site; early nodulin; Vicia hirsuta; Vicia faba
Erscheinungsjahr
2000
Zeitschriftentitel
PLANT SCIENCE
Band
160
Ausgabe
1
Seite(n)
67-75
ISSN
0168-9452
Page URI
https://pub.uni-bielefeld.de/record/1618110

Zitieren

Fruhling M, Schroder G, Hohnjec N, Pühler A, Perlick AM, Küster H. The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE. 2000;160(1):67-75.
Fruhling, M., Schroder, G., Hohnjec, N., Pühler, A., Perlick, A. M., & Küster, H. (2000). The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE, 160(1), 67-75. https://doi.org/10.1016/S0168-9452(00)00362-9
Fruhling, M, Schroder, G, Hohnjec, N, Pühler, Alfred, Perlick, AM, and Küster, Helge. 2000. “The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules”. PLANT SCIENCE 160 (1): 67-75.
Fruhling, M., Schroder, G., Hohnjec, N., Pühler, A., Perlick, A. M., and Küster, H. (2000). The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE 160, 67-75.
Fruhling, M., et al., 2000. The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE, 160(1), p 67-75.
M. Fruhling, et al., “The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules”, PLANT SCIENCE, vol. 160, 2000, pp. 67-75.
Fruhling, M., Schroder, G., Hohnjec, N., Pühler, A., Perlick, A.M., Küster, H.: The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE. 160, 67-75 (2000).
Fruhling, M, Schroder, G, Hohnjec, N, Pühler, Alfred, Perlick, AM, and Küster, Helge. “The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules”. PLANT SCIENCE 160.1 (2000): 67-75.

8 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean.
Nanjareddy K, Arthikala MK, Aguirre AL, Gómez BM, Lara M., J Vis Exp (130), 2017
PMID: 29364203
Loss of the nodule-specific cysteine rich peptide, NCR169, abolishes symbiotic nitrogen fixation in the Medicago truncatula dnf7 mutant.
Horváth B, Domonkos Á, Kereszt A, Szűcs A, Ábrahám E, Ayaydin F, Bóka K, Chen Y, Chen R, Murray JD, Udvardi MK, Kondorosi É, Kaló P., Proc Natl Acad Sci U S A 112(49), 2015
PMID: 26401023
Plant and fungal gene expression in mycorrhizal protocorms of the orchid Serapias vomeracea colonized by Tulasnella calospora.
Balestrini R, Nerva L, Sillo F, Girlanda M, Perotto S., Plant Signal Behav 9(11), 2014
PMID: 25482758
Temporal and spatial control of gene expression in horticultural crops.
Dutt M, Dhekney SA, Soriano L, Kandel R, Grosser JW., Hortic Res 1(), 2014
PMID: 26504550
The putative phytocyanin genes in Chinese cabbage (Brassica rapa L.): genome-wide identification, classification and expression analysis.
Li J, Gao G, Zhang T, Wu X., Mol Genet Genomics 288(1-2), 2013
PMID: 23212439
Characterization of transcriptome dynamics during watermelon fruit development: sequencing, assembly, annotation and gene expression profiles.
Guo S, Liu J, Zheng Y, Huang M, Zhang H, Gong G, He H, Ren Y, Zhong S, Fei Z, Xu Y., BMC Genomics 12(), 2011
PMID: 21936920
The phytocyanin gene family in rice (Oryza sativa L.): genome-wide identification, classification and transcriptional analysis.
Ma H, Zhao H, Liu Z, Zhao J., PLoS One 6(10), 2011
PMID: 21984902
A semi-quantitative RT-PCR method to measure the in vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression.
Meadus WJ., Biol Proced Online 5(), 2003
PMID: 12734559

46 References

Daten bereitgestellt von Europe PubMed Central.

Identification of two alfalfa early nodulin genes with homology to members of the pea Enod12 gene family.
Allison LA, Kiss GB, Bauer P, Poiret M, Pierre M, Savoure A, Kondorosi E, Kondorosi A., Plant Mol. Biol. 21(2), 1993
PMID: 7678770
Alfalfa Enod12 genes are differentially regulated during nodule development by Nod factors and Rhizobium invasion.
Bauer P, Crespi MD, Szecsi J, Allison LA, Schultze M, Ratet P, Kondorosi E, Kondorosi A., Plant Physiol. 105(2), 1994
PMID: 8066132
Nod factors and cytokinins induce cortical cell division, amyloplast deposition and MsEnod12a expression patterns in alfalfa roots
Bauer, Plant J. 10(), 1996
Development of the legume root nodule.
Brewin NJ., Annu. Rev. Cell Biol. 7(), 1991
PMID: 1809347
A novel type of DNA-binding protein interacts with a conserved sequence in an early nodulin ENOD12 promoter.
Christiansen H, Hansen AC, Vijn I, Pallisgaard N, Larsen K, Yang WC, Bisseling T, Marcker KA, Jensen EO., Plant Mol. Biol. 32(5), 1996
PMID: 8980533
ENOD12, an early nodulin gene, is not required for nodule formation and efficient nitrogen fixation in alfalfa.
Csanadi G, Szecsi J, Kalo P, Kiss P, Endre G, Kondorosi A, Kondorosi E, Kiss GB., Plant Cell 6(2), 1994
PMID: 8148645
Cloned nodulin genes for symbiotic nitrogen fixation
Delauney, Plant Mol. Biol. Rep. 6(), 1988
Rhizobium--plant signal exchange.
Fisher RF, Long SR., Nature 357(6380), 1992
PMID: 1614514
Developmental aspects of the Rhizobium-legume symbiosis.
Franssen HJ, Vijn I, Yang WC, Bisseling T., Plant Mol. Biol. 19(1), 1992
PMID: 1600171
Lambda replacement vectors carrying polylinker sequences.
Frischauf AM, Lehrach H, Poustka A, Murray N., J. Mol. Biol. 170(4), 1983
PMID: 6315951
The Vicia faba leghemoglobin gene VfLb29 is induced in root nodules and in roots colonized by the arbuscular mycorrhizal fungus Glomus fasciculatum
Frühling, Mol. Plant-Microbe Interact. 10(), 1997
Signal transduction in Rhizobium-induced nodule formation.
Geurts R, Franssen H., Plant Physiol. 112(2), 1996
PMID: 8883373
Characterization of the pea ENOD12B gene and expression analyses of the two ENOD12 genes in nodule, stem and flower tissue.
Govers F, Harmsen H, Heidstra R, Michielsen P, Prins M, van Kammen A, Bisseling T., Mol. Gen. Genet. 228(1-2), 1991
PMID: 1715970
VsENBP1 regulates the expression of the early nodulin PsENOD12B
Hansen, Plant Mol. Biol. 1999(), 1999
Nod factor-induced host responses and mechanisms of nod factor perception.
Heidstra R, Bisseling T., New Phytol. 133(1), 1996
PMID: IND20632245
Characterization of a proline-rich cell wall protein gene family of soybean. A comparative analysis.
Hong JC, Nagao RT, Key JL., J. Biol. Chem. 265(5), 1990
PMID: 2303411
Lipo-oligosaccharides of Rhizobium induce infection-related early nodulin gene expression in pea root hairs.
Horvath B, Heidstra R, Lados M, Moerman M, Spaink HP, Prome JC, van Kammen A, Bisseling T., Plant J. 4(4), 1993
PMID: 8252073
Construction and screening cDNA libraries in lambda gt10 and lambda gt11
Huynh, 1985
The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules.
Kuster H, Quandt HJ, Broer I, Perlick AM, Puhler A., Plant Mol. Biol. 29(4), 1995
PMID: 8541502
The modular nodulins Nvf-28/32 of broad bean (Vicia faba L.): alternative exon combinations account for different modular structures.
Kuster H, Fruhling M, Puhler A, Perlick AM., Mol. Gen. Genet. 252(6), 1996
PMID: 8917307
A gene that encodes a proline-rich nodulin with limited homology to PsENOD12 is expressed in the invasion zone of Rhizobium meliloti-induced alfalfa root nodules.
Lobler M, Hirsch AM., Plant Physiol. 103(1), 1993
PMID: 8208847
Symbiotic Nitrogen Fixation.
Mylona P, Pawlowski K, Bisseling T., Plant Cell 7(7), 1995
PMID: 12242391
Nodule-specific gene expression
Pawlowski, Phys. Plantarum 99(), 1997
A survey of transcripts expressed specifically in root nodules of broadbean (Vicia faba L.).
Perlick AM, Puhler A., Plant Mol. Biol. 22(6), 1993
PMID: 8400140
The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)8-barrel-type seed proteins.
Perlick AM, Fruhling M, Schroder G, Frosch SC, Puhler A., Plant Physiol. 110(1), 1996
PMID: 8587978
Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa.
Pichon M, Journet EP, Dedieu A, de Billy F, Truchet G, Barker DG., Plant Cell 4(10), 1992
PMID: 1446169
ENOD12 gene expression as a molecular marker for comparing Rhizobium-dependent and -independent nodulation in alfalfa
Pichon, Mol. Plant-Microbe Interact. 7(), 1994
Genes involved in lipopolysaccharide production and symbiosis are clustered on the chromosome of Rhizobium leguminosarum biovar viciae VF39.
Priefer UB., J. Bacteriol. 171(11), 1989
PMID: 2553672
Transgenic root nodules of Vicia hirsuta: A fast and efficient system for the study of gene expression in indeterminate-type nodules
Quandt, Mol. Plant-Microbe Interact. 6(), 1993

Sambrook, 1989
The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction.
Scheres B, Van De Wiel C, Zalensky A, Horvath B, Spaink H, Van Eck H, Zwartkruis F, Wolters AM, Gloudemans T, Van Kammen A., Cell 60(2), 1990
PMID: 2297789
Sequential induction of nodulin gene expression in the developing pea nodule.
Scheres B, van Engelen F, van der Knaap E, van de Wiel C, van Kammen A, Bisseling T., Plant Cell 2(8), 1990
PMID: 2152123
The temporal and spatial transcription pattern in root nodules of Vicia faba nodulin genes encoding glycine-rich proteins.
Schroder G, Fruhling M, Puhler A, Perlick AM., Plant Mol. Biol. 33(1), 1997
PMID: 9037164
What makes nodulation signals host-plant specific?
Schultze M, Kondorosi A., Trends Microbiol. 3(10), 1995
PMID: 8564354
Structure and function of plant cell wall proteins.
Showalter AM., Plant Cell 5(1), 1993
PMID: 8439747
A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in gram negative bacteria
Simon, Biotechnology 1(), 1983
The current status and portability of our sequence handling software
Staden, Nucl. Acids Res. 14(), 1986
Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation.
Vancanneyt G, Schmidt R, O'Connor-Sanchez A, Willmitzer L, Rocha-Sosa M., Mol. Gen. Genet. 220(2), 1990
PMID: 2325623
Suggested nomenclature for plant genes involved in nodulation and symbiosis
van, Plant Mol. Biol. Rep. 2(), 1984
Root nodule development: origin, function and regulation of nodulin genes
Verma, Phys. Plantarum 85(), 1992
VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots.
Vijn I, Yang WC, Pallisgard N, Ostergaard Jensen E, van Kammen A, Bisseling T., Plant Mol. Biol. 28(6), 1995
PMID: 7548828
A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and nod factor induced expression.
Vijn I, Christiansen H, Lauridsen P, Kardailsky I, Quandt HJ, Broer I, Drenth J, Ostergaard Jensen E, van Kammen A, Bisseling T., Plant Mol. Biol. 28(6), 1995
PMID: 7548827
Topogenic signals in integral membrane proteins
von, Eur. J. Biochem. 174(), 1992
A new proline-rich early nodulin from Medicago truncatula is highly expressed in nodule meristematic cells.
Wilson RC, Long F, Maruoka EM, Cooper JB., Plant Cell 6(9), 1994
PMID: 7919991
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
Yanisch-Perron C, Vieira J, Messing J., Gene 33(1), 1985
PMID: 2985470
A simplified protocol for fast plasmid DNA sequencing
Zimmermann, Nucl. Acids Res. 18(), 1990
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 11164578
PubMed | Europe PMC

Suchen in

Google Scholar