Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis

Lagares A, Hozbor DF, Niehaus K, Otero AJLP, Lorenzen J, Arnold W, Pühler A (2001)
J Bacteriol 183(4): 1248-1258.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Lagares, A; Hozbor, DF; Niehaus, KarstenUniBi; Otero, AJLP; Lorenzen, J; Arnold, WalterUniBi; Pühler, AlfredUniBi
Einrichtung
Centrum für Biotechnologie
Fakultät für Biologie > Proteom- und Metabolomforschung
Abstract / Bemerkung
The genetic characterization of a 5.5-kb chromosomal region of Sinorhizobium meliloti 2011 that contains lpsB, a gene required for the normal development of symbiosis with Medicago spp., is presented. The nucleotide sequence of this DNA fragment revealed the presence of six genes: greA and lpsB, transcribed in the forward direction; and lpsE, lpsD, lpsC, and lrp, transcribed in the reverse direction. Except for lpsB, none of the lps genes were relevant for nodulation and nitrogen fixation. Analysis of the transcriptional organization of lpsB showed that greA and lpsB are part of separate transcriptional units, which is in agreement with the finding of a DNA stretch homologous to a "nonnitrogen" promoter consensus sequence between greA and lpsB. The opposite orientation of lpsB with respect to its first downstream coding sequence, lpsE, indicated that the altered LPS and the defective symbiosis of lpsB mutants are both consequences of a primary nonpolar defect in a single gene. Global sequence comparisons revealed that the greA-lpsB and lrp genes of S. meliloti have a genetic organization similar to that of their homologous loci in R. leguminosarum by. viciae. In particular, high sequence similarity was found between the translation product of lpsB and a core related biosynthetic mannosyltransferase of R. leguminosarum by. viciae encoded by the lpcC gene. The functional relationship between these two genes was demonstrated in genetic complementation experiments in which the S. meliloti lpsB gene restored the wild-type LPS phenotype when introduced into lpcC mutants of R. leguminosarum. These results support the view that S. meliloti lpsB also encodes a mannosyltransferase that participates in the biosynthesis of the LPS core. Evidence is provided for the presence of other lpsB-homologous sequences in several members of the family Rhizobiaceae.
Erscheinungsjahr
2001
Zeitschriftentitel
J Bacteriol
Band
183
Ausgabe
4
Seite(n)
1248-1258
ISSN
0021-9193
Page URI
https://pub.uni-bielefeld.de/record/1618037

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Lagares A, Hozbor DF, Niehaus K, et al. Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis. J Bacteriol. 2001;183(4):1248-1258.
Lagares, A., Hozbor, D. F., Niehaus, K., Otero, A. J. L. P., Lorenzen, J., Arnold, W., & Pühler, A. (2001). Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis. J Bacteriol, 183(4), 1248-1258. https://doi.org/10.1128/JB.183.4.1248-1258.2001
Lagares, A, Hozbor, DF, Niehaus, Karsten, Otero, AJLP, Lorenzen, J, Arnold, Walter, and Pühler, Alfred. 2001. “Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis”. J Bacteriol 183 (4): 1248-1258.
Lagares, A., Hozbor, D. F., Niehaus, K., Otero, A. J. L. P., Lorenzen, J., Arnold, W., and Pühler, A. (2001). Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis. J Bacteriol 183, 1248-1258.
Lagares, A., et al., 2001. Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis. J Bacteriol, 183(4), p 1248-1258.
A. Lagares, et al., “Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis”, J Bacteriol, vol. 183, 2001, pp. 1248-1258.
Lagares, A., Hozbor, D.F., Niehaus, K., Otero, A.J.L.P., Lorenzen, J., Arnold, W., Pühler, A.: Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis. J Bacteriol. 183, 1248-1258 (2001).
Lagares, A, Hozbor, DF, Niehaus, Karsten, Otero, AJLP, Lorenzen, J, Arnold, Walter, and Pühler, Alfred. “Genetic characterization of a Sinorhizobium meliloti chromosomal region involved in lipopolysaccharide biosynthesis”. J Bacteriol 183.4 (2001): 1248-1258.

19 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Genome-Wide Sensitivity Analysis of the Microsymbiont Sinorhizobium meliloti to Symbiotically Important, Defensin-Like Host Peptides.
Arnold MFF, Shabab M, Penterman J, Boehme KL, Griffitts JS, Walker GC., MBio 8(4), 2017
PMID: 28765224
Transcriptional regulator LsrB of Sinorhizobium meliloti positively regulates the expression of genes involved in lipopolysaccharide biosynthesis.
Tang G, Wang Y, Luo L., Appl Environ Microbiol 80(17), 2014
PMID: 24951786
Queuosine biosynthesis is required for sinorhizobium meliloti-induced cytoskeletal modifications on HeLa Cells and symbiosis with Medicago truncatula.
Marchetti M, Capela D, Poincloux R, Benmeradi N, Auriac MC, Le Ru A, Maridonneau-Parini I, Batut J, Masson-Boivin C., PLoS One 8(2), 2013
PMID: 23409119
The Sinorhizobium meliloti essential porin RopA1 is a target for numerous bacteriophages.
Crook MB, Draper AL, Guillory RJ, Griffitts JS., J Bacteriol 195(16), 2013
PMID: 23749981
The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules.
Margaret I, Lucas MM, Acosta-Jurado S, Buendía-Clavería AM, Fedorova E, Hidalgo Á, Rodríguez-Carvajal MA, Rodriguez-Navarro DN, Ruiz-Sainz JE, Vinardell JM., PLoS One 8(10), 2013
PMID: 24098345
Synthesis of the flavonoid-induced lipopolysaccharide of Rhizobium Sp. strain NGR234 requires rhamnosyl transferases encoded by genes rgpF and wbgA.
Ardissone S, Noel KD, Klement M, Broughton WJ, Deakin WJ., Mol Plant Microbe Interact 24(12), 2011
PMID: 22066901
Identification and characterization of a glycosyltransferase involved in Acinetobacter baumannii lipopolysaccharide core biosynthesis.
Luke NR, Sauberan SL, Russo TA, Beanan JM, Olson R, Loehfelm TW, Cox AD, St Michael F, Vinogradov EV, Campagnari AA., Infect Immun 78(5), 2010
PMID: 20194587
Phage specificity and lipopolysaccarides of stem- and root-nodulating bacteria (Azorhizobium caulinodans, Sinorhizobium spp., and Rhizobium spp.) of Sesbania spp.
Sharma RS, Mishra V, Mohmmed A, Babu CR., Arch Microbiol 189(4), 2008
PMID: 17989956
Cloning and complementation analysis of greA gene involved in salt tolerance of Sinorhizobium meliloti.
Wei W, Gu ZJ, Zhang B, Wang L, Yang SS., Ann Microbiol 57(2), 2007
PMID: IND43996177
Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis.
D'Antuono AL, Casabuono A, Couto A, Ugalde RA, Lepek VC., Mol Plant Microbe Interact 18(5), 2005
PMID: 15915643
Isolation of salt-sensitive mutants from Sinorhizobium meliloti and characterization of genes involved in salt tolerance.
Wei W, Jiang J, Li X, Wang L, Yang SS., Lett Appl Microbiol 39(3), 2004
PMID: 15287875
Involvement of exo5 in production of surface polysaccharides in Rhizobium leguminosarum and its role in nodulation of Vicia sativa subsp. nigra.
Laus MC, Logman TJ, Van Brussel AA, Carlson RW, Azadi P, Gao MY, Kijne JW., J Bacteriol 186(19), 2004
PMID: 15375143
The symbiotic defect in a Sinorhizobium meliloti lipopolysaccharide mutant can be overcome by expression of other surface polysaccharides.
Hozbor DF, Pich Otero AJ, Lodeiro AR, Del Papa MF, Pistorio M, Lagares A., Res Microbiol 155(10), 2004
PMID: 15567281
Sinorhizobium meliloti acpXL mutant lacks the C28 hydroxylated fatty acid moiety of lipid A and does not express a slow migrating form of lipopolysaccharide.
Sharypova LA, Niehaus K, Scheidle H, Holst O, Becker A., J Biol Chem 278(15), 2003
PMID: 12566460
Relaxed sugar donor selectivity of a Sinorhizobium meliloti ortholog of the Rhizobium leguminosarum mannosyl transferase LpcC. Role of the lipopolysaccharide core in symbiosis of Rhizobiaceae with plants.
Kanipes MI, Kalb SR, Cotter RJ, Hozbor DF, Lagares A, Raetz CR., J Biol Chem 278(18), 2003
PMID: 12591936
A mannosyl transferase required for lipopolysaccharide inner core assembly in Rhizobium leguminosarum. Purification, substrate specificity, and expression in Salmonella waaC mutants.
Kanipes MI, Ribeiro AA, Lin S, Cotter RJ, Raetz CR., J Biol Chem 278(18), 2003
PMID: 12591937
Surface polysaccharide involvement in establishing the rhizobium-legume symbiosis.
Fraysse N, Couderc F, Poinsot V., Eur J Biochem 270(7), 2003
PMID: 12653992
Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants.
Campbell GR, Sharypova LA, Scheidle H, Jones KM, Niehaus K, Becker A, Walker GC., J Bacteriol 185(13), 2003
PMID: 12813079
Chronic intracellular infection of alfalfa nodules by Sinorhizobium meliloti requires correct lipopolysaccharide core.
Campbell GR, Reuhs BL, Walker GC., Proc Natl Acad Sci U S A 99(6), 2002
PMID: 11904442

62 References

Daten bereitgestellt von Europe PubMed Central.

A family of high-copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing.
Arnold W, Puhler A., Gene 70(1), 1988
PMID: 2907323
Specific oligosaccharide form of the Rhizobium meliloti exopolysaccharide promotes nodule invasion in alfalfa.
Battisti L, Lara JC, Leigh JA., Proc. Natl. Acad. Sci. U.S.A. 89(12), 1992
PMID: 1608972
Identification and analysis of the Rhizobium meliloti exoAMONP genes involved in exopolysaccharide biosynthesis and mapping of promoters located on the exoHKLAMONP fragment.
Becker A, Kleickmann A, Keller M, Arnold W, Puhler A., Mol. Gen. Genet. 241(3-4), 1993
PMID: 8246891
Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT, and exoI involved in exopolysaccharide biosynthesis and nodule invasion: exoU and exoW probably encode glucosyltransferases.
Becker A, Kleickmann A, Kuster H, Keller M, Arnold W, Puhler A., Mol. Plant Microbe Interact. 6(6), 1993
PMID: 8118055
New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions.
Becker A, Schmidt M, Jager W, Puhler A., Gene 162(1), 1995
PMID: 7557413
R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098
Genes and signal molecules involved in the rhizobia-leguminoseae symbiosis.
Bladergroen MR, Spaink HP., Curr. Opin. Plant Biol. 1(4), 1998
PMID: 10066605
Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon.
Blatny JM, Brautaset T, Winther-Larsen HC, Haugan K, Valla S., Appl. Environ. Microbiol. 63(2), 1997
PMID: 9023917
GreA protein: a transcription elongation factor from Escherichia coli.
Borukhov S, Polyakov A, Nikiforov V, Goldfarb A., Proc. Natl. Acad. Sci. U.S.A. 89(19), 1992
PMID: 1384037
XL1-Blue: a high efficient plasmid transforming strain with beta-galactosidase selection
Bullock W, Fernandez J, Short J., 1987
Different phenotypic classes of mutants defective in synthesis of K antigen
Campbell G, Reuhs B, Walker G., 1999
Identification and characterization of large plasmids in using agarose gel electrophoresis
Casse F, Boucher C, Julliot J, Michell M, Dénarié J., 1979
Identification of Agrobacterium tumefaciens genes that direct the complete catabolism of octopine.
Cho K, Fuqua C, Martin BS, Winans SC., J. Bacteriol. 178(7), 1996
PMID: 8606160
One-hour downward alkaline capillary transfer for blotting of DNA and RNA.
Chomczynski P., Anal. Biochem. 201(1), 1992
PMID: 1621951
Lipopolysaccharide mutants of Rhizobium meliloti are not defective in symbiosis.
Clover RH, Kieber J, Signer ER., J. Bacteriol. 171(7), 1989
PMID: 2738026
Rhizobium lipopolysaccharide modulates infection thread development in white clover root hairs.
Dazzo FB, Truchet GL, Hollingsworth RI, Hrabak EM, Pankratz HS, Philip-Hollingsworth S, Salzwedel JL, Chapman K, Appenzeller L, Squartini A., J. Bacteriol. 173(17), 1991
PMID: 1885517
Isolation and characterization of alfalfa-nodulating rhizobia present in acidic soils of central argentina and uruguay
del Papa MF , Balague LJ, Sowinski SC, Wegener C, Segundo E, Abarca FM, Toro N, Niehaus K, P hler A , Aguilar OM, Martinez-Drets G, Lagares A., Appl. Environ. Microbiol. 65(4), 1999
PMID: 10103231
Isolation and characterization of mutants of Rhizobium leguminosarum bv. viciae 248 with altered lipopolysaccharides: possible role of surface charge or hydrophobicity in bacterial release from the infection thread.
de Maagd RA, Rao AS, Mulders IH, Goosen-de Roo L, van Loosdrecht MC, Wijffelman CA, Lugtenberg BJ., J. Bacteriol. 171(2), 1989
PMID: 2536673
Nitrogen fixation ability of exopolysaccharide synthesis mutants of Rhizobium sp. strain NGR234 and Rhizobium trifolii is restored by the addition of homologous exopolysaccharides.
Djordjevic SP, Chen H, Batley M, Redmond JW, Rolfe BG., J. Bacteriol. 169(1), 1987
PMID: 3025187
Structural characterization of the O-antigenic polysaccharide of the lipopolysaccharide from Rhizobium etli strain CE3. A unique O-acetylated glycan of discrete size, containing 3-O-methyl-6-deoxy-L-talose and 2,3,4-tri-O-,methyl-l fucose.
Forsberg LS, Bhat UR, Carlson RW., J. Biol. Chem. 275(25), 2000
PMID: 10858446
The structures of the lipopolysaccharides from Rhizobium etli strains CE358 and CE359. The complete structure of the core region of R. etli lipopolysaccharides.
Forsberg LS, Carlson RW., J. Biol. Chem. 273(5), 1998
PMID: 9446581
Low molecular weight EPS II of Rhizobium meliloti allows nodule invasion in Medicago sativa.
Gonzalez JE, Reuhs BL, Walker GC., Proc. Natl. Acad. Sci. U.S.A. 93(16), 1996
PMID: 8710923
Genetic mapping of symbiotic loci on the Rhizobium meliloti chromosome.
Glazebrook J, Meiri G, Walker GC., Mol. Plant Microbe Interact. 5(3), 1992
PMID: 1330089
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
Henikoff S., Gene 28(3), 1984
PMID: 6235151
Structural studies of a novel exopolysaccharide produced by a mutant of Rhizobium meliloti strain Rm1021.
Her GR, Glazebrook J, Walker GC, Reinhold VN., Carbohydr. Res. 198(2), 1990
PMID: 2379191
Recovery of Tn5-flanking bacterial DNA by vector-mediated walking from the transposon to the host genome.
Hozbor DF, Pich Otero AJ, Wynne ME, Petruccelli S, Lagares A., Anal. Biochem. 259(2), 1998
PMID: 9618212
Nitrogen fixation in leguminous plants. I. General characters of root nodule bacteria isolated from species of and in Australia
Jensen H., 1942
Cloning and overexpression of glycosyltransferases that generate the lipopolysaccharide core of Rhizobium leguminosarum.
Kadrmas JL, Allaway D, Studholme RE, Sullivan JT, Ronson CW, Poole PS, Raetz CR., J. Biol. Chem. 273(41), 1998
PMID: 9756877
Lipopolysaccharide core glycosylation in Rhizobium leguminosarum. An unusual mannosyl transferase resembling the heptosyl transferase I of Escherichia coli.
Kadrmas JL, Brozek KA, Raetz CR., J. Biol. Chem. 271(50), 1996
PMID: 8943265
Lipopolysaccharides and K-antigens: their structures, biosynthesis, and functions
Kannenberg E, Reuhs B, Forsberg L, Carlson R., 1998
Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular polysaccharide production and invasion of the symbiotic nodule: the rkpK gene encodes a UDP-glucose dehydrogenase.
Kereszt A, Kiss E, Reuhs BL, Carlson RW, Kondorosi A, Putnoky P., J. Bacteriol. 180(20), 1998
PMID: 9765575
Identification of the gene in and its role in regulation of δ-aminolevulinic acid uptake J
King N, O'Brian M., 1997
A directional, high-frequency chromosomal mobilization system for genetic mapping of Rhizobium meliloti.
Klein S, Lohman K, Clover R, Walker GC, Signer ER., J. Bacteriol. 174(1), 1992
PMID: 1309521
A Rhizobium meliloti lipopolysaccharide mutant altered in competitiveness for nodulation of alfalfa.
Lagares A, Caetano-Anolles G, Niehaus K, Lorenzen J, Ljunggren HD, Puhler A, Favelukes G., J. Bacteriol. 174(18), 1992
PMID: 1325969

Maniatis T, Fritsch E, Sambrook J., 1982

Miller J., 1972
Leucine-responsive regulatory protein: a global regulator of gene expression in E. coli.
Newman EB, Lin R., Annu. Rev. Microbiol. 49(), 1995
PMID: 8561478
Recent advances in the study of nod factor perception and signal transduction.
Niebel A, Gressent F, Bono JJ, Ranjeva R, Cullimore J., Biochimie 81(6), 1999
PMID: 10433121
Symbiotic suppression of the Medicago sativa plant defense system by oligosaccharides
Niehaus K, Albus U, Baier R, Schiene K, Schröder S, Pühler A., 1998
The role of microbial surface polysaccharides in the Rhizobium-legume interaction.
Niehaus K, Becker A., Subcell. Biochem. 29(), 1998
PMID: 9594645
A lipopolysaccharide mutant induces effective nodules on the host plant (alfalfa) but fails to establish a symbiosis with
Niehaus K, Lagares A, Pühler A., 1998
Mutations in Rhizobium phaseoli that lead to arrested development of infection threads.
Noel KD, Vandenbosch KA, Kulpaca B., J. Bacteriol. 168(3), 1986
PMID: 3782040
Alfalfa root nodule invasion efficiency is dependent on Sinorhizobium meliloti polysaccharides.
Pellock BJ, Cheng HP, Walker GC., J. Bacteriol. 182(15), 2000
PMID: 10894742
Genes involved in lipopolysaccharide production and symbiosis are clustered on the chromosome of Rhizobium leguminosarum biovar viciae VF39.
Priefer UB., J. Bacteriol. 171(11), 1989
PMID: 2553672
Rhizobium meliloti lipopolysaccharide and exopolysaccharide can have the same function in the plant-bacterium interaction.
Putnoky P, Petrovics G, Kereszt A, Grosskopf E, Ha DT, Banfalvi Z, Kondorosi A., J. Bacteriol. 172(9), 1990
PMID: 2168384
Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021.
Reinhold BB, Chan SY, Reuber TL, Marra A, Walker GC, Reinhold VN., J. Bacteriol. 176(7), 1994
PMID: 8144468
Rhizobium fredii and Rhizobium meliloti produce 3-deoxy-D-manno-2-octulosonic acid-containing polysaccharides that are structurally analogous to group II K antigens (capsular polysaccharides) found in Escherichia coli.
Reuhs BL, Carlson RW, Kim JS., J. Bacteriol. 175(11), 1993
PMID: 8501061
Sinorhizobium fredii and Sinorhizobium meliloti produce structurally conserved lipopolysaccharides and strain-specific K antigens.
Reuhs BL, Geller DP, Kim JS, Fox JE, Kolli VS, Pueppke SG., Appl. Environ. Microbiol. 64(12), 1998
PMID: 9835585
Epitope identification for a panel of anti-Sinorhizobium meliloti monoclonal antibodies and application to the analysis of K antigens and lipopolysaccharides from bacteroids.
Reuhs BL, Stephens SB, Geller DP, Kim JS, Glenn J, Przytycki J, Ojanen-Reuhs T., Appl. Environ. Microbiol. 65(11), 1999
PMID: 10543844
Suppression of the Fix- phenotype of Rhizobium meliloti exoB mutants by lpsZ is correlated to a modified expression of the K polysaccharide.
Reuhs BL, Williams MN, Kim JS, Carlson RW, Cote F., J. Bacteriol. 177(15), 1995
PMID: 7635814
Genes involved in the carbon metabolism of bacteriods
Ronson C, Astwood P., 1985
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum.
Schafer A, Tauch A, Jager W, Kalinowski J, Thierbach G, Puhler A., Gene 145(1), 1994
PMID: 8045426
Regulation of symbiotic root nodule development.
Schultze M, Kondorosi A., Annu. Rev. Genet. 32(), 1998
PMID: 9928474
A broad host range mobilization system for genetic engeneering:transposon mutagenesis in gram-negative bacteria
Simon R, Priefer U, Pühler A., 1983
A lipopolysaccharide mutant of Bradyrhizobium japonicum that uncouples plant from bacterial differentiation.
Stacey G, So JS, Roth LE, Lakshmi SK B, Carlson RW., Mol. Plant Microbe Interact. 4(4), 1991
PMID: 1799697
The current status and portability of our sequence handling software.
Staden R., Nucleic Acids Res. 14(1), 1986
PMID: 3511446
Exogenous suppression of the symbiotic deficiencies of Rhizobium meliloti exo mutants.
Urzainqui A, Walker GC., J. Bacteriol. 174(10), 1992
PMID: 1577707
Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis.
Valverde C, Hozbor DF, Lagares A., BioTechniques 22(2), 1997
PMID: 9043688
Identification of a plasmid-borne locus in Rhizobium etli KIM5s involved in lipopolysaccharide O-chain biosynthesis and nodulation of Phaseolus vulgaris.
Vinuesa P, Reuhs BL, Breton C, Werner D., J. Bacteriol. 181(18), 1999
PMID: 10482500
Structural characterization of the symbiotically important low-molecular-weight succinoglycan of Sinorhizobium meliloti.
Wang LX, Wang Y, Pellock B, Walker GC., J. Bacteriol. 181(21), 1999
PMID: 10542182
Rhizobium meliloti genes required for C4-dicarboxylate transport and symbiotic nitrogen fixation are located on a megaplasmid.
Watson RJ, Chan YK, Wheatcroft R, Yang AF, Han SH., J. Bacteriol. 170(2), 1988
PMID: 2828335
A simplified protocol for fast plasmid DNA sequencing.
Zimmermann J, Voss H, Schwager C, Stegemann J, Erfle H, Stucky K, Kristensen T, Ansorge W., Nucleic Acids Res. 18(4), 1990
PMID: 2315028
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