The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules

Becker JD, Moreira LM, Kapp D, Frosch SC, Pühler A, Perlick AM (2001)
PLANT MOLECULAR BIOLOGY 47(6): 749-759.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Recently we described the novel nodulin gene VfENOD18, whose corresponding transcripts were restricted to the nitrogen-fixing zone III of broad bean root nodules. To characterize VfENOD18 on the protein level, polyclonal antibodies were generated using the purified recombinant VfENOD18 protein produced in Escherichia coli by employing the pMAL-c expression system. These antibodies recognized immunoreactive proteins isolated from indeterminate nodules of different leguminous plants, but also from non-symbiotic tissues of Glycine max and from tissues of Arabidopsis thaliana and Zea mays. Using immunogold labelling the nodulin VfENOD18 was localized to the cytoplasm of infected cells in the nitrogen-fixing zone of broad bean nodules. Due to the homology of the VfENOD18 sequence to that of the ATP-binding protein MJ0577 from the hyperthermophile Methanococcus jannaschii the recombinant VfENOD18 protein was tested for ATP-binding. Using the biotin photoaffinity ATP analogue 8N(3)ATP[gamma ]biotin it could be demonstrated that VfENOD18 is an ATP-binding protein. PCR experiments revealed that the amino acid sequences of the putative C-terminal ATP-binding sites of the VfENOD18 homologues from Lens culinaris, Vicia hirsuta, Vicia sativa and Vicia villosa were conserved. We propose that VfENOD18 is a member of a novel family of ATP-binding proteins in plants.
Erscheinungsjahr
Zeitschriftentitel
PLANT MOLECULAR BIOLOGY
Band
47
Ausgabe
6
Seite(n)
749-759
ISSN
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Becker JD, Moreira LM, Kapp D, Frosch SC, Pühler A, Perlick AM. The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. PLANT MOLECULAR BIOLOGY. 2001;47(6):749-759.
Becker, J. D., Moreira, L. M., Kapp, D., Frosch, S. C., Pühler, A., & Perlick, A. M. (2001). The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. PLANT MOLECULAR BIOLOGY, 47(6), 749-759. doi:10.1023/A:1013664311052
Becker, J. D., Moreira, L. M., Kapp, D., Frosch, S. C., Pühler, A., and Perlick, A. M. (2001). The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. PLANT MOLECULAR BIOLOGY 47, 749-759.
Becker, J.D., et al., 2001. The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. PLANT MOLECULAR BIOLOGY, 47(6), p 749-759.
J.D. Becker, et al., “The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules”, PLANT MOLECULAR BIOLOGY, vol. 47, 2001, pp. 749-759.
Becker, J.D., Moreira, L.M., Kapp, D., Frosch, S.C., Pühler, A., Perlick, A.M.: The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. PLANT MOLECULAR BIOLOGY. 47, 749-759 (2001).
Becker, JD, Moreira, LM, Kapp, D, Frosch, SC, Pühler, Alfred, and Perlick, AM. “The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules”. PLANT MOLECULAR BIOLOGY 47.6 (2001): 749-759.

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PMID: 16884822
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36 References

Daten bereitgestellt von Europe PubMed Central.

Correlation between ultrastructural differentiation of bacteroids and nitrogen fixation in alfalfa nodules.
Vasse J, de Billy F, Camut S, Truchet G., J. Bacteriol. 172(8), 1990
PMID: 2376562
A phylogeny of the chloroplast gene rbcL in the Leguminosae: taxonomic correlations and insights into the evolution of nodulation.
Doyle J, Doyle J, Ballenger J, Dickson E, Kajita T, Ohashi H., Am. J. Bot. 84(4), 1997
PMID: 21708606
An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein.
Maina CV, Riggs PD, Grandea AG 3rd, Slatko BE, Moran LS, Tagliamonte JA, McReynolds LA, Guan CD., Gene 74(2), 1988
PMID: 3073105
Analysis of Medicago truncatula nodule expressed sequence tags.
Gyorgyey J, Vaubert D, Jimenez-Zurdo JI, Charon C, Troussard L, Kondorosi A, Kondorosi E., Mol. Plant Microbe Interact. 13(1), 2000
PMID: 10656586
Gene structure and differential regulation of the Rhizobium-induced peroxidase gene rip1.
Peng HM, Dreyer DA, VandenBosch KA, Cook D., Plant Physiol. 112(4), 1996
PMID: 8972593
Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): a role in hormone-mediated cell division?
McKhann HI, Frugier F, Petrovics G, de la Pena TC, Jurkevitch E, Brown S, Kondorosi E, Kondorosi A, Crespi M., Plant Mol. Biol. 34(5), 1997
PMID: 9278167
An improved assay for nanomole amounts of inorganic phosphate.
Lanzetta PA, Alvarez LJ, Reinach PS, Candia OA., Anal. Biochem. 100(1), 1979
PMID: 161695
JPred: a consensus secondary structure prediction server.
Cuff JA, Clamp ME, Siddiqui AS, Finlay M, Barton GJ., Bioinformatics 14(10), 1998
PMID: 9927721
Alfalfa Enod12 genes are differentially regulated during nodule development by Nod factors and Rhizobium invasion.
Bauer P, Crespi MD, Szecsi J, Allison LA, Schultze M, Ratet P, Kondorosi E, Kondorosi A., Plant Physiol. 105(2), 1994
PMID: 8066132
The AAA team: related ATPases with diverse functions.
Patel S, Latterich M., Trends Cell Biol. 8(2), 1998
PMID: 9695811
Regulation of symbiotic root nodule development.
Schultze M, Kondorosi A., Annu. Rev. Genet. 32(), 1998
PMID: 9928474
Symbiotic induction of a MADS-box gene during development of alfalfa root nodules.
Heard J, Dunn K., Proc. Natl. Acad. Sci. U.S.A. 92(12), 1995
PMID: 7777496
Development of the legume root nodule.
Brewin NJ., Annu. Rev. Cell Biol. 7(), 1991
PMID: 1809347
Localization of H(+)-ATPases in soybean root nodules.
Fedorova E, Thomson R, Whitehead LF, Maudoux O, Udvardi MK, Day DA., Planta 209(1), 1999
PMID: 10467028
Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics.
Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH., Proc. Natl. Acad. Sci. U.S.A. 95(26), 1998
PMID: 9860944
Rhizobium meliloti Nod factors elicit cell-specific transcription of the ENOD12 gene in transgenic alfalfa.
Journet EP, Pichon M, Dedieu A, de Billy F, Truchet G, Barker DG., Plant J. 6(2), 1994
PMID: 7920714
The evolution of nodulation.
Gualtieri G, Bisseling T., Plant Mol. Biol. 42(1), 2000
PMID: 10688136
The broad bean nodulin VfENOD18 is a member of a novel family of plant proteins with homologies to the bacterial MJ0577 superfamily.
Hohnjec N, Kuster H, Albus U, Frosch SC, Becker JD, Puhler A, Perlick AM, Fruhling M., Mol. Gen. Genet. 264(3), 2000
PMID: 11085263
Symbiotic Nitrogen Fixation.
Mylona P, Pawlowski K, Bisseling T., Plant Cell 7(7), 1995
PMID: 12242391
Genes and signal molecules involved in the rhizobia-leguminoseae symbiosis.
Bladergroen MR, Spaink HP., Curr. Opin. Plant Biol. 1(4), 1998
PMID: 10066605
Mechanism of action of the lexA gene product.
Brent R, Ptashne M., Proc. Natl. Acad. Sci. U.S.A. 78(7), 1981
PMID: 7027256
A plant regulator controlling development of symbiotic root nodules.
Schauser L, Roussis A, Stiller J, Stougaard J., Nature 402(6758), 1999
PMID: 10647012
GTPase-activating proteins: helping hands to complement an active site.
Scheffzek K, Ahmadian MR, Wittinghofer A., Trends Biochem. Sci. 23(7), 1998
PMID: 9697416

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