Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes

Krüger O, Ladewig J, Köster K, Ragg H (2002)
GENE 293(1-2): 97-105.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Krüger, Olaf; Ladewig, Julia; Köster, Katharina; Ragg, HermannUniBi
Abstract / Bemerkung
By applying homology-search and text-mining programs we have found that the Drosophila serine protease inhibitor (serpin) gene sp4 harbours four reactive centre-coding exons. The mutually exclusive use of these cassettes in combination with alternatively selectable exons at the 5'-end or in the 3'-untransiated region of the gene allows generation of more than ten different transcripts, all of which are expressed in Drosophila embryos. These transcripts may code for eight different Sp4 protein isoforms with different biological functions, which dependent on the splice pattern - either may be secreted. reside in the endoplasmic reticulum, or may be located in the cytoplasm. An examination revealed the presence of two serpin genes, each coding for two or three likely alternative reactive centre exon cassettes, respectively, also in the Caenorhabditis elegans genome, The occurrence of such serpin genes in some groups of metazoa reflects a parsimonious way to enlarge the adaptive ability of these organisms to cope with a plethora of different serine and cysteine proteases. (C) 2002 Elsevier Science B.V. All rights reserved.
exon/intron structured; evolution; alternative splicing; elegans; Caenorhabditis; serine protease inhibitors; serpin; Drosophila melanogaster
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Krüger O, Ladewig J, Köster K, Ragg H. Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes. GENE. 2002;293(1-2):97-105.
Krüger, O., Ladewig, J., Köster, K., & Ragg, H. (2002). Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes. GENE, 293(1-2), 97-105. https://doi.org/10.1016/S0378-1119(02)00697-2
Krüger, Olaf, Ladewig, Julia, Köster, Katharina, and Ragg, Hermann. 2002. “Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes”. GENE 293 (1-2): 97-105.
Krüger, O., Ladewig, J., Köster, K., and Ragg, H. (2002). Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes. GENE 293, 97-105.
Krüger, O., et al., 2002. Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes. GENE, 293(1-2), p 97-105.
O. Krüger, et al., “Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes”, GENE, vol. 293, 2002, pp. 97-105.
Krüger, O., Ladewig, J., Köster, K., Ragg, H.: Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes. GENE. 293, 97-105 (2002).
Krüger, Olaf, Ladewig, Julia, Köster, Katharina, and Ragg, Hermann. “Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and nematodes”. GENE 293.1-2 (2002): 97-105.

32 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

iTRAQ proteomic analysis of the interactions between Bombyx mori nuclear polyhedrosis virus and silkworm.
Dong WT, Xiao LF, Hu JJ, Zhao XX, Liu JX, Zhang Y., J Proteomics 166(), 2017
PMID: 28755911
Rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment.
Rodriguez-Valle M, Xu T, Kurscheid S, Lew-Tabor AE., Parasit Vectors 8(), 2015
PMID: 25564202
Antibacterial activity of serine protease inhibitor 1 from kuruma shrimp Marsupenaeus japonicus.
Zhao YR, Xu YH, Jiang HS, Xu S, Zhao XF, Wang JX., Dev Comp Immunol 44(2), 2014
PMID: 24412215
Sequencing, De Novo assembly and annotation of the Colorado Potato Beetle, Leptinotarsa decemlineata, Transcriptome.
Kumar A, Congiu L, Lindström L, Piiroinen S, Vidotto M, Grapputo A., PLoS One 9(1), 2014
PMID: 24465841
High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster.
Ellisdon AM, Zhang Q, Henstridge MA, Johnson TK, Warr CG, Law RH, Whisstock JC., BMC Struct Biol 14(), 2014
PMID: 24758516
Repression of liver colorectal metastasis by the serpin Spn4A a naturally occurring inhibitor of the constitutive secretory proprotein convertases.
Sfaxi F, Scamuffa N, Lalou C, Ma J, Metrakos P, Siegfried G, Ragg H, Bikfalvi A, Calvo F, Khatib AM., Oncotarget 5(12), 2014
PMID: 24961901
The roles of serpins in mosquito immunology and physiology.
Gulley MM, Zhang X, Michel K., J Insect Physiol 59(2), 2013
PMID: 22960307
Protease inhibitors and proteolytic signalling cascades in insects.
Gubb D, Sanz-Parra A, Barcena L, Troxler L, Fullaondo A., Biochimie 92(12), 2010
PMID: 20850496
A comparative analysis of serpin genes in the silkworm genome.
Zou Z, Picheng Z, Weng H, Mita K, Jiang H., Genomics 93(4), 2009
PMID: 19150649
The serpin gene family in Anopheles gambiae.
Suwanchaichinda C, Kanost MR., Gene 442(1-2), 2009
PMID: 19394412
Multiple gains of spliceosomal introns in a superfamily of vertebrate protease inhibitor genes.
Ragg H, Kumar A, Köster K, Bentele C, Wang Y, Frese MA, Prib N, Krüger O., BMC Evol Biol 9(), 2009
PMID: 19698129
Identification and analysis of serpin-family genes by homology and synteny across the 12 sequenced Drosophilid genomes.
Garrett M, Fullaondo A, Troxler L, Micklem G, Gubb D., BMC Genomics 10(), 2009
PMID: 19849829
Ancestry and evolution of a secretory pathway serpin.
Kumar A, Ragg H., BMC Evol Biol 8(), 2008
PMID: 18793432
The Spn4 gene from Drosophila melanogaster is a multipurpose defence tool directed against proteases from three different peptidase families.
Brüning M, Lummer M, Bentele C, Smolenaars MM, Rodenburg KW, Ragg H., Biochem J 401(1), 2007
PMID: 16989645
Overexpression and altered nucleocytoplasmic distribution of Anopheles ovalbumin-like SRPN10 serpins in Plasmodium-infected midgut cells.
Danielli A, Barillas-Mury C, Kumar S, Kafatos FC, Loukeris TG., Cell Microbiol 7(2), 2005
PMID: 15659062
Tip of another iceberg: Drosophila serpins.
Reichhart JM., Trends Cell Biol 15(12), 2005
PMID: 16260136
SRP-2 is a cross-class inhibitor that participates in postembryonic development of the nematode Caenorhabditis elegans: initial characterization of the clade L serpins.
Pak SC, Kumar V, Tsu C, Luke CJ, Askew YS, Askew DJ, Mills DR, Brömme D, Silverman GA., J Biol Chem 279(15), 2004
PMID: 14739286
Isolation, characterization, and recombinant expression of multiple serpins from the cat flea, Ctenocephalides felis.
Brandt KS, Silver GM, Becher AM, Gaines PJ, Maddux JD, Jarvis EE, Wisnewski N., Arch Insect Biochem Physiol 55(4), 2004
PMID: 15027074
The Spn4 gene of Drosophila encodes a potent furin-directed secretory pathway serpin.
Richer MJ, Keays CA, Waterhouse J, Minhas J, Hashimoto C, Jean F., Proc Natl Acad Sci U S A 101(29), 2004
PMID: 15247425
Serpins in unicellular Eukarya, Archaea, and Bacteria: sequence analysis and evolution.
Roberts TH, Hejgaard J, Saunders NF, Cavicchioli R, Curmi PM., J Mol Evol 59(4), 2004
PMID: 15638455
Inhibition of furin by serpin Spn4A from Drosophila melanogaster.
Oley M, Letzel MC, Ragg H., FEBS Lett 577(1-2), 2004
PMID: 15527779
The chicken pituitary expresses an ovoinhibitor-like protein in subpopulations of some, but not all, hormone-producing cell types.
Oubre CM, D'Hondt E, Moore RW, Hargis BM, Berghman LR., Domest Anim Endocrinol 25(4), 2003
PMID: 14652138
A novel family of anticoagulants from the saliva of Ixodes scapularis.
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E., Insect Mol Biol 11(6), 2002
PMID: 12421422

31 References

Daten bereitgestellt von Europe PubMed Central.

The genome sequence of Drosophila melanogaster.
Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, George RA, Lewis SE, Richards S, Ashburner M, Henderson SN, Sutton GG, Wortman JR, Yandell MD, Zhang Q, Chen LX, Brandon RC, Rogers YH, Blazej RG, Champe M, Pfeiffer BD, Wan KH, Doyle C, Baxter EG, Helt G, Nelson CR, Gabor GL, Abril JF, Agbayani A, An HJ, Andrews-Pfannkoch C, Baldwin D, Ballew RM, Basu A, Baxendale J, Bayraktaroglu L, Beasley EM, Beeson KY, Benos PV, Berman BP, Bhandari D, Bolshakov S, Borkova D, Botchan MR, Bouck J, Brokstein P, Brottier P, Burtis KC, Busam DA, Butler H, Cadieu E, Center A, Chandra I, Cherry JM, Cawley S, Dahlke C, Davenport LB, Davies P, de Pablos B, Delcher A, Deng Z, Mays AD, Dew I, Dietz SM, Dodson K, Doup LE, Downes M, Dugan-Rocha S, Dunkov BC, Dunn P, Durbin KJ, Evangelista CC, Ferraz C, Ferriera S, Fleischmann W, Fosler C, Gabrielian AE, Garg NS, Gelbart WM, Glasser K, Glodek A, Gong F, Gorrell JH, Gu Z, Guan P, Harris M, Harris NL, Harvey D, Heiman TJ, Hernandez JR, Houck J, Hostin D, Houston KA, Howland TJ, Wei MH, Ibegwam C, Jalali M, Kalush F, Karpen GH, Ke Z, Kennison JA, Ketchum KA, Kimmel BE, Kodira CD, Kraft C, Kravitz S, Kulp D, Lai Z, Lasko P, Lei Y, Levitsky AA, Li J, Li Z, Liang Y, Lin X, Liu X, Mattei B, McIntosh TC, McLeod MP, McPherson D, Merkulov G, Milshina NV, Mobarry C, Morris J, Moshrefi A, Mount SM, Moy M, Murphy B, Murphy L, Muzny DM, Nelson DL, Nelson DR, Nelson KA, Nixon K, Nusskern DR, Pacleb JM, Palazzolo M, Pittman GS, Pan S, Pollard J, Puri V, Reese MG, Reinert K, Remington K, Saunders RD, Scheeler F, Shen H, Shue BC, Siden-Kiamos I, Simpson M, Skupski MP, Smith T, Spier E, Spradling AC, Stapleton M, Strong R, Sun E, Svirskas R, Tector C, Turner R, Venter E, Wang AH, Wang X, Wang ZY, Wassarman DA, Weinstock GM, Weissenbach J, Williams SM, WoodageT , Worley KC, Wu D, Yang S, Yao QA, Ye J, Yeh RF, Zaveri JS, Zhan M, Zhang G, Zhao Q, Zheng L, Zheng XH, Zhong FN, Zhong W, Zhou X, Zhu S, Zhu X, Smith HO, Gibbs RA, Myers EW, Rubin GM, Venter JC., Science 287(5461), 2000
PMID: 10731132
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
Arabidopsis Genome Initiative., Nature 408(6814), 2000
PMID: 11130711
Phylogenetic analyses of amino acid variation in the serpin proteins.
Atchley WR, Lokot T, Wollenberg K, Dress A, Ragg H., Mol. Biol. Evol. 18(8), 2001
PMID: 11470841
Genome sequence of the nematode C. elegans: a platform for investigating biology.
C. elegans Sequencing Consortium., Science 282(5396), 1998
PMID: 9851916
Genome-wide analysis of the Drosophila immune response by using oligonucleotide microarrays.
De Gregorio E, Spellman PT, Rubin GM, Lemaitre B., Proc. Natl. Acad. Sci. U.S.A. 98(22), 2001
PMID: 11606746
Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.
Emanuelsson O, Nielsen H, Brunak S, von Heijne G., J. Mol. Biol. 300(4), 2000
PMID: 10891285
The PROSITE database, its status in 2002.
Falquet L, Pagni M, Bucher P, Hulo N, Sigrist CJ, Hofmann K, Bairoch A., Nucleic Acids Res. 30(1), 2002
PMID: 11752303
A novel Drosophila serpin that inhibits serine proteases.
Han J, Zhang H, Min G, Kemler D, Hashimoto C., FEBS Lett. 468(2-3), 2000
PMID: 10692585
A genome-wide analysis of immune responses in Drosophila.
Irving P, Troxler L, Heuer TS, Belvin M, Kopczynski C, Reichhart JM, Hoffmann JA, Hetru C., Proc. Natl. Acad. Sci. U.S.A. 98(26), 2001
PMID: 11742098
Mutually exclusive exon use and reactive center diversity in insect serpins.
Jiang H, Wang Y, Kanost MR., J. Biol. Chem. 269(1), 1994
PMID: 8276850
Organization of serpin gene-1 from Manduca sexta. Evolution of a family of alternate exons encoding the reactive site loop.
Jiang H, Wang Y, Huang Y, Mulnix AB, Kadel J, Cole K, Kanost MR., J. Biol. Chem. 271(45), 1996
PMID: 8910411
Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila.
Levashina EA, Langley E, Green C, Gubb D, Ashburner M, Hoffmann JA, Reichhart JM., Science 285(5435), 1999
PMID: 10489372
Complete sequence of the cDNA for human alpha 1-antitrypsin and the gene for the S variant.
Long GL, Chandra T, Woo SL, Davie EW, Kurachi K., Biochemistry 23(21), 1984
PMID: 6093867
Hsp47: a collagen-specific molecular chaperone.
Nagata K., Trends Biochem. Sci. 21(1), 1996
PMID: 8848834
The retention signal for soluble proteins of the endoplasmic reticulum.
Pelham HR., Trends Biochem. Sci. 15(12), 1990
PMID: 2077689
The serpin superfamily of proteinase inhibitors: structure, function, and regulation.
Potempa J, Korzus E, Travis J., J. Biol. Chem. 269(23), 1994
PMID: 8206889
Vertebrate serpins: construction of a conflict-free phylogeny by combining exon-intron and diagnostic site analyses.
Ragg H, Lokot T, Kamp PB, Atchley WR, Dress A., Mol. Biol. Evol. 18(4), 2001
PMID: 11264410
Comparative genomics of the eukaryotes.
Rubin GM, Yandell MD, Wortman JR, Gabor Miklos GL, Nelson CR, Hariharan IK, Fortini ME, Li PW, Apweiler R, Fleischmann W, Cherry JM, Henikoff S, Skupski MP, Misra S, Ashburner M, Birney E, Boguski MS, Brody T, Brokstein P, Celniker SE, Chervitz SA, Coates D, Cravchik A, Gabrielian A, Galle RF, Gelbart WM, George RA, Goldstein LS, Gong F, Guan P, Harris NL, Hay BA, Hoskins RA, Li J, Li Z, Hynes RO, Jones SJ, Kuehl PM, Lemaitre B, Littleton JT, Morrison DK, Mungall C, O'Farrell PH, Pickeral OK, Shue C, Vosshall LB, Zhang J, Zhao Q, Zheng XH, Lewis S., Science 287(5461), 2000
PMID: 10731134
The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. Evolution, mechanism of inhibition, novel functions, and a revised nomenclature.
Silverman GA, Bird PI, Carrell RW, Church FC, Coughlin PB, Gettins PG, Irving JA, Lomas DA, Luke CJ, Moyer RW, Pemberton PA, Remold-O'Donnell E, Salvesen GS, Travis J, Whisstock JC., J. Biol. Chem. 276(36), 2001
PMID: 11435447
What do dysfunctional serpins tell us about molecular mobility and disease?
Stein PE, Carrell RW., Nat. Struct. Biol. 2(2), 1995
PMID: 7749926
Serpins in the Caenorhabditis elegans genome.
Whisstock JC, Irving JA, Bottomley SP, Pike RN, Lesk AM., Proteins 36(1), 1999
PMID: 10373004
Agrep – a fast approximate pattern-matching tool
Wu, 1992
The serpin inhibitory mechanism is critically dependent on the length of the reactive center loop.
Zhou A, Carrell RW, Huntington JA., J. Biol. Chem. 276(29), 2001
PMID: 11325972

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