The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi

Hohnjec N, Perlick AM, Pühler A, Küster H (2003)
Mol Plant Microbe Interact 16(10): 903-915.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ;
Abstract / Bemerkung
The MtSucS1 gene encodes a sucrose synthase (EC 2.4.1.13) in the model legume Medicago truncatula. To determine the expression pattern of this gene in different organs and in particular during root endosymbioses, we transformed M. truncatula with specific regions of MtSucS1 fused to the gusAint reporter gene. These fusions directed an induction to the vasculature of leaves, stems, and roots as well as to flowers, developing seeds, young pods, and germinating seedlings. In root nodules, strong promoter activity occurred in the infected cells of the nitrogen-fixing zone but was additionally observed in the meristematic region, the prefixing zone, and the inner cortex, including the vasculature. Concerning endomycorrhizal roots, the MtSucS1 promoter mediated strongest expression in cortical cells harboring arbuscules. Specifically in highly colonized root sections, GUS-staining was furthermore detected in the surrounding cortical cells, irrespective of a direct contact with fungal structures. In accordance with the presence of an orthologous PsSus1 gene, we observed a comparable regulation of MtSucS1 expression in the grain legume Pisum sativum in response to microbial symbionts. Unlike other members of the MtSucS gene family, the presence of rhizobial or Glomus microsymbionts significantly altered and enhanced MtSucS1 gene expression, leading us to propose that MtSucS1 is involved in generating sink-strength, not only in root nodules but also in mycorrhizal roots.
Stichworte
transgenic Medicago truncatula; plants; assimilate partitioning; phloem loading
Erscheinungsjahr
2003
Zeitschriftentitel
Mol Plant Microbe Interact
Band
16
Ausgabe
10
Seite(n)
903-915
ISSN
0894-0282
Page URI
https://pub.uni-bielefeld.de/record/1610193

Zitieren

Hohnjec N, Perlick AM, Pühler A, Küster H. The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi. Mol Plant Microbe Interact. 2003;16(10):903-915.
Hohnjec, N., Perlick, A. M., Pühler, A., & Küster, H. (2003). The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi. Mol Plant Microbe Interact, 16(10), 903-915. doi:10.1094/MPMI.2003.16.10.903
Hohnjec, N., Perlick, A. M., Pühler, A., and Küster, H. (2003). The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi. Mol Plant Microbe Interact 16, 903-915.
Hohnjec, N., et al., 2003. The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi. Mol Plant Microbe Interact, 16(10), p 903-915.
N. Hohnjec, et al., “The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi”, Mol Plant Microbe Interact, vol. 16, 2003, pp. 903-915.
Hohnjec, N., Perlick, A.M., Pühler, A., Küster, H.: The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi. Mol Plant Microbe Interact. 16, 903-915 (2003).
Hohnjec, N., Perlick, A. M., Pühler, Alfred, and Küster, Helge. “The Medicago truncatula sucrose synthase gene MtSucS1 is activated both in the infected region of root nodules and in the cortex of roots colonized by arbuscular mycorrhizal fungi”. Mol Plant Microbe Interact 16.10 (2003): 903-915.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 14558692
PubMed | Europe PMC

Suchen in

Google Scholar