Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection
Schotter J, Kamp P-B, Becker A, Pühler A, Reiss G, Brückl H (2004)
BIOSENSORS & BIOELECTRONICS 19(10): 1149-1156.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Schotter, Jörg;
Kamp, Paul-BertramUniBi;
Becker, Anke;
Pühler, AlfredUniBi 
;
Reiss, GünterUniBi ;
Brückl, Hubert
;
Reiss, GünterUniBi ;
Brückl, HubertEinrichtung
Abstract / Bemerkung
We present a comparative analysis of a magnetoresistive biosensor to standard fluorescent DNA detection. The biosensor consists of giant magnetoresistive (GMR) type Cu/Ni80Fe20 multilayers in the second antiferromagnetic coupling maximum. Each of the 206 elements of the magnetoresistive biosensor is patterned into a spiral-shaped line that can cover the area of a typical DNA spot (70 mum diameter). The probe DNA is assembled on top of the sensor elements in different concentrations ranging from 16 pg/mul to 10 ng/mul. Complementary biotin-labeled analyte DNA is hybridized to the probe DNA at a concentration of 10 ng/mul. A number of different commercially available magnetic microspheres are investigated to determine the most appropriate markers. The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations. (C) 2003 Elsevier B.V. All rights reserved.
Stichworte
microarray;
magnetic markers;
fluorescence;
biosensor;
magnetoresistive;
sensors;
DNA
Erscheinungsjahr
2004
Zeitschriftentitel
BIOSENSORS & BIOELECTRONICS
Band
19
Ausgabe
10
Seite(n)
1149-1156
ISSN
0956-5663
Page URI
https://pub.uni-bielefeld.de/record/1608095
Zitieren
Schotter J, Kamp P-B, Becker A, Pühler A, Reiss G, Brückl H. Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection. BIOSENSORS & BIOELECTRONICS. 2004;19(10):1149-1156.
Schotter, J., Kamp, P. - B., Becker, A., Pühler, A., Reiss, G., & Brückl, H. (2004). Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection. BIOSENSORS & BIOELECTRONICS, 19(10), 1149-1156. https://doi.org/10.1016/j.bios.2003.11.007
Schotter, Jörg, Kamp, Paul-Bertram, Becker, Anke, Pühler, Alfred, Reiss, Günter, and Brückl, Hubert. 2004. “Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection”. BIOSENSORS & BIOELECTRONICS 19 (10): 1149-1156.
Schotter, J., Kamp, P. - B., Becker, A., Pühler, A., Reiss, G., and Brückl, H. (2004). Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection. BIOSENSORS & BIOELECTRONICS 19, 1149-1156.
Schotter, J., et al., 2004. Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection. BIOSENSORS & BIOELECTRONICS, 19(10), p 1149-1156.
J. Schotter, et al., “Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection”, BIOSENSORS & BIOELECTRONICS, vol. 19, 2004, pp. 1149-1156.
Schotter, J., Kamp, P.-B., Becker, A., Pühler, A., Reiss, G., Brückl, H.: Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection. BIOSENSORS & BIOELECTRONICS. 19, 1149-1156 (2004).
Schotter, Jörg, Kamp, Paul-Bertram, Becker, Anke, Pühler, Alfred, Reiss, Günter, and Brückl, Hubert. “Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection”. BIOSENSORS & BIOELECTRONICS 19.10 (2004): 1149-1156.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
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Wang J, Xu D, Erdem A, Polsky R, Salazar MA., Talanta 56(5), 2002
PMID: 18968572
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