A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa

Schiene K, Donath S, Brecht M, Pühler A, Niehaus K (2004)
Molecular Genetics and Genomics 272(1): 57-66.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Schiene, K.; Donath, S.; Brecht, M.; Pühler, AlfredUniBi ; Niehaus, KarstenUniBi
Abstract / Bemerkung
Rab-related small GTP-binding proteins are known to be involved in the regulation of the vesicular transport system in eucaryotic cells. In this paper we report the isolation of the cDNA clone MS- rab11f from Medicago sativa (alfalfa) root nodules using a combination of RT-PCR and SSCP analysis. MS- rab11f shows high homology to the Rab-related cDNA clone LJ- rab11f from Lotus japonicus root nodules. The MS-Rab11F protein expressed in Escherichia coli was found to bind GTP, confirming that the isolated cDNA indeed codes for a small GTP-binding protein. Expression analysis by RT-PCR demonstrated that MS- rab11f is preferentially expressed in root nodules of alfalfa. Using the cDNA-sequence of MS-rab11f, a peptide-specific antibody was generated. Western blot analysis with this antibody revealed that two Rab11F isoforms, designated MS-Rab11FA and MS-Rab11FB, are found in M. sativa root nodules.
Stichworte
root nodules; protein; single-strand conformational polymorphism (SSCP); Rab-related; GTP-binding protein; Medicago sativa
Erscheinungsjahr
2004
Zeitschriftentitel
Molecular Genetics and Genomics
Band
272
Ausgabe
1
Seite(n)
57-66
ISSN
1617-4615
eISSN
1617-4623
Page URI
https://pub.uni-bielefeld.de/record/1606843

Zitieren

Schiene K, Donath S, Brecht M, Pühler A, Niehaus K. A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics. 2004;272(1):57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., & Niehaus, K. (2004). A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics, 272(1), 57-66. doi:10.1007/s00438-004-1029-1
Schiene, K., Donath, S., Brecht, M., Pühler, A., and Niehaus, K. (2004). A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics 272, 57-66.
Schiene, K., et al., 2004. A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics, 272(1), p 57-66.
K. Schiene, et al., “A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa”, Molecular Genetics and Genomics, vol. 272, 2004, pp. 57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., Niehaus, K.: A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics. 272, 57-66 (2004).
Schiene, K., Donath, S., Brecht, M., Pühler, Alfred, and Niehaus, Karsten. “A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa”. Molecular Genetics and Genomics 272.1 (2004): 57-66.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Molecular and phylogenetic characterization of the sieve element occlusion gene family in Fabaceae and non-Fabaceae plants.
Rüping B, Ernst AM, Jekat SB, Nordzieke S, Reineke AR, Müller B, Bornberg-Bauer E, Prüfer D, Noll GA., BMC Plant Biol 10(), 2010
PMID: 20932300
The role of Rab GTPases in cell wall metabolism.
Lycett G., J Exp Bot 59(15), 2008
PMID: 18945942

32 References

Daten bereitgestellt von Europe PubMed Central.

Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell?
Lazar T, Gotte M, Gallwitz D., Trends Biochem. Sci. 22(12), 1997
PMID: 9433126
GTPases: multifunctional molecular switches regulating vesicular traffic.
Nuoffer C, Balch WE., Annu. Rev. Biochem. 63(), 1994
PMID: 7979258
Isoprenoid modification of rab proteins terminating in CC or CXC motifs.
Khosravi-Far R, Lutz RJ, Cox AD, Conroy L, Bourne JR, Sinensky M, Balch WE, Buss JE, Der CJ., Proc. Natl. Acad. Sci. U.S.A. 88(14), 1991
PMID: 1648736
Alfalfa and tobacco cells react differently to chitin oligosaccharides and sinorhizobium meliloti nodulation factors
Baier R, Schiene K, Kohring B, Flaschel E, Niehaus K., Planta 210(1), 1999
PMID: 10592044
Cloning and characterization of rac-like cDNAs from Arabidopsis thaliana.
Winge P, Brembu T, Bones AM., Plant Mol. Biol. 35(4), 1997
PMID: 9349271
Distinct localization of two closely related Ypt3/Rab11 proteins on the trafficking pathway in higher plants.
Inaba T, Nagano Y, Nagasaki T, Sasaki Y., J. Biol. Chem. 277(11), 2001
PMID: 11756458
Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.
Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T., Proc. Natl. Acad. Sci. U.S.A. 86(8), 1989
PMID: 2565038
The Arabidopsis Rab GTPase family: another enigma variation.
Rutherford S, Moore I., Curr. Opin. Plant Biol. 5(6), 2002
PMID: 12393015
GTP-binding proteins in plants.
Bischoff F, Molendijk A, Rajendrakumar CS, Palme K., Cell. Mol. Life Sci. 55(2), 1999
PMID: 10188584
Nitric oxide functions as a signal in plant disease resistance.
Delledonne M, Xia Y, Dixon RA, Lamb C., Nature 394(6693), 1998
PMID: 9707120
Rha1, an Arabidopsis Rab5 homolog, plays a critical role in the vacuolar trafficking of soluble cargo proteins.
Sohn EJ, Kim ES, Zhao M, Kim SJ, Kim H, Kim YW, Lee YJ, Hillmer S, Sohn U, Jiang L, Hwang I., Plant Cell 15(5), 2003
PMID: 12724533
The GTPase superfamily: conserved structure and molecular mechanism.
Bourne HR, Sanders DA, McCormick F., Nature 349(6305), 1991
PMID: 1898771
Analysis of the small GTPase gene superfamily of Arabidopsis.
Vernoud V, Horton AC, Yang Z, Nielsen E., Plant Physiol. 131(3), 2003
PMID: 12644670
Rab11, a small GTPase associated with both constitutive and regulated secretory pathways in PC12 cells.
Urbe S, Huber LA, Zerial M, Tooze SA, Parton RG., FEBS Lett. 334(2), 1993
PMID: 8224244
Location of light-repressible, small GTP-binding protein of the YPT/rab family in the growing zone of etiolated pea stems.
Nagano Y, Okada Y, Narita H, Asaka Y, Sasaki Y., Proc. Natl. Acad. Sci. U.S.A. 92(14), 1995
PMID: 7603989
Biogenesis of the peribacteroid membrane in root nodules.
Verma DP, Hong Z., Trends Microbiol. 4(9), 1996
PMID: 8885172
A cDNA cloning method for monomeric GTP-binding proteins by ligand blotting.
Nagano Y, Matsuno R, Sasaki Y., Anal. Biochem. 211(2), 1993
PMID: 8317694
The diversity of Rab proteins in vesicle transport.
Novick P, Zerial M., Curr. Opin. Cell Biol. 9(4), 1997
PMID: 9261061

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