A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa
Schiene K, Donath S, Brecht M, Pühler A, Niehaus K (2004)
Molecular Genetics and Genomics 272(1): 57-66.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Einrichtung
Abstract / Bemerkung
Rab-related small GTP-binding proteins are known to be involved in the regulation of the vesicular transport system in eucaryotic cells. In this paper we report the isolation of the cDNA clone MS- rab11f from Medicago sativa (alfalfa) root nodules using a combination of RT-PCR and SSCP analysis. MS- rab11f shows high homology to the Rab-related cDNA clone LJ- rab11f from Lotus japonicus root nodules. The MS-Rab11F protein expressed in Escherichia coli was found to bind GTP, confirming that the isolated cDNA indeed codes for a small GTP-binding protein. Expression analysis by RT-PCR demonstrated that MS- rab11f is preferentially expressed in root nodules of alfalfa. Using the cDNA-sequence of MS-rab11f, a peptide-specific antibody was generated. Western blot analysis with this antibody revealed that two Rab11F isoforms, designated MS-Rab11FA and MS-Rab11FB, are found in M. sativa root nodules.
Stichworte
root nodules;
protein;
single-strand conformational polymorphism (SSCP);
Rab-related;
GTP-binding protein;
Medicago sativa
Erscheinungsjahr
2004
Zeitschriftentitel
Molecular Genetics and Genomics
Band
272
Ausgabe
1
Seite(n)
57-66
ISSN
1617-4615
eISSN
1617-4623
Page URI
https://pub.uni-bielefeld.de/record/1606843
Zitieren
Schiene K, Donath S, Brecht M, Pühler A, Niehaus K. A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics. 2004;272(1):57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., & Niehaus, K. (2004). A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics, 272(1), 57-66. https://doi.org/10.1007/s00438-004-1029-1
Schiene, K., Donath, S., Brecht, M., Pühler, Alfred, and Niehaus, Karsten. 2004. “A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa”. Molecular Genetics and Genomics 272 (1): 57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., and Niehaus, K. (2004). A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics 272, 57-66.
Schiene, K., et al., 2004. A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics, 272(1), p 57-66.
K. Schiene, et al., “A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa”, Molecular Genetics and Genomics, vol. 272, 2004, pp. 57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., Niehaus, K.: A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics. 272, 57-66 (2004).
Schiene, K., Donath, S., Brecht, M., Pühler, Alfred, and Niehaus, Karsten. “A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa”. Molecular Genetics and Genomics 272.1 (2004): 57-66.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
4 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Molecular and phylogenetic characterization of the sieve element occlusion gene family in Fabaceae and non-Fabaceae plants.
Rüping B, Ernst AM, Jekat SB, Nordzieke S, Reineke AR, Müller B, Bornberg-Bauer E, Prüfer D, Noll GA., BMC Plant Biol 10(), 2010
PMID: 20932300
Rüping B, Ernst AM, Jekat SB, Nordzieke S, Reineke AR, Müller B, Bornberg-Bauer E, Prüfer D, Noll GA., BMC Plant Biol 10(), 2010
PMID: 20932300
A small GTPase of the Rab family is required for root hair formation and preinfection stages of the common bean-Rhizobium symbiotic association.
Blanco FA, Meschini EP, Zanetti ME, Aguilar OM., Plant Cell 21(9), 2009
PMID: 19749154
Blanco FA, Meschini EP, Zanetti ME, Aguilar OM., Plant Cell 21(9), 2009
PMID: 19749154
Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules.
Yuksel B, Memon AR., J Exp Bot 59(14), 2008
PMID: 18849296
Yuksel B, Memon AR., J Exp Bot 59(14), 2008
PMID: 18849296
32 References
Daten bereitgestellt von Europe PubMed Central.
Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell?
Lazar T, Gotte M, Gallwitz D., Trends Biochem. Sci. 22(12), 1997
PMID: 9433126
Lazar T, Gotte M, Gallwitz D., Trends Biochem. Sci. 22(12), 1997
PMID: 9433126
The N-myristoylated Rab-GTPase m-Rabmc is involved in post-Golgi trafficking events to the lytic vacuole in plant cells.
Bolte S, Brown S, Satiat-Jeunemaitre B., J. Cell. Sci. 117(Pt 6), 2004
PMID: 14762108
Bolte S, Brown S, Satiat-Jeunemaitre B., J. Cell. Sci. 117(Pt 6), 2004
PMID: 14762108
Roles of plant homologs of Rab1p and Rab7p in the biogenesis of the peribacteroid membrane, a subcellular compartment formed de novo during root nodule symbiosis.
Cheon CI, Lee NG, Siddique AB, Bal AK, Verma DP., EMBO J. 12(11), 1993
PMID: 8223429
Cheon CI, Lee NG, Siddique AB, Bal AK, Verma DP., EMBO J. 12(11), 1993
PMID: 8223429
GTPases: multifunctional molecular switches regulating vesicular traffic.
Nuoffer C, Balch WE., Annu. Rev. Biochem. 63(), 1994
PMID: 7979258
Nuoffer C, Balch WE., Annu. Rev. Biochem. 63(), 1994
PMID: 7979258
Isoprenoid modification of rab proteins terminating in CC or CXC motifs.
Khosravi-Far R, Lutz RJ, Cox AD, Conroy L, Bourne JR, Sinensky M, Balch WE, Buss JE, Der CJ., Proc. Natl. Acad. Sci. U.S.A. 88(14), 1991
PMID: 1648736
Khosravi-Far R, Lutz RJ, Cox AD, Conroy L, Bourne JR, Sinensky M, Balch WE, Buss JE, Der CJ., Proc. Natl. Acad. Sci. U.S.A. 88(14), 1991
PMID: 1648736
Alfalfa and tobacco cells react differently to chitin oligosaccharides and sinorhizobium meliloti nodulation factors
Baier R, Schiene K, Kohring B, Flaschel E, Niehaus K., Planta 210(1), 1999
PMID: 10592044
Baier R, Schiene K, Kohring B, Flaschel E, Niehaus K., Planta 210(1), 1999
PMID: 10592044
Cloning and characterization of rac-like cDNAs from Arabidopsis thaliana.
Winge P, Brembu T, Bones AM., Plant Mol. Biol. 35(4), 1997
PMID: 9349271
Winge P, Brembu T, Bones AM., Plant Mol. Biol. 35(4), 1997
PMID: 9349271
A rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants.
Batoko H, Zheng HQ, Hawes C, Moore I., Plant Cell 12(11), 2000
PMID: 11090219
Batoko H, Zheng HQ, Hawes C, Moore I., Plant Cell 12(11), 2000
PMID: 11090219
Distinct localization of two closely related Ypt3/Rab11 proteins on the trafficking pathway in higher plants.
Inaba T, Nagano Y, Nagasaki T, Sasaki Y., J. Biol. Chem. 277(11), 2001
PMID: 11756458
Inaba T, Nagano Y, Nagasaki T, Sasaki Y., J. Biol. Chem. 277(11), 2001
PMID: 11756458
Involvement of small GTP-binding proteins in defense signal-transduction pathways of higher plants.
Sano H, Ohashi Y., Proc. Natl. Acad. Sci. U.S.A. 92(10), 1995
PMID: 11607540
Sano H, Ohashi Y., Proc. Natl. Acad. Sci. U.S.A. 92(10), 1995
PMID: 11607540
Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.
Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T., Proc. Natl. Acad. Sci. U.S.A. 86(8), 1989
PMID: 2565038
Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T., Proc. Natl. Acad. Sci. U.S.A. 86(8), 1989
PMID: 2565038
The Arabidopsis Rab GTPase family: another enigma variation.
Rutherford S, Moore I., Curr. Opin. Plant Biol. 5(6), 2002
PMID: 12393015
Rutherford S, Moore I., Curr. Opin. Plant Biol. 5(6), 2002
PMID: 12393015
Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotus japonicus, and expression of corresponding mRNAs in developing root nodules.
Borg S, Brandstrup B, Jensen TJ, Poulsen C., Plant J. 11(2), 1997
PMID: 9076991
Borg S, Brandstrup B, Jensen TJ, Poulsen C., Plant J. 11(2), 1997
PMID: 9076991
Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.
Kyhse-Andersen J., J. Biochem. Biophys. Methods 10(3-4), 1984
PMID: 6530509
Kyhse-Andersen J., J. Biochem. Biophys. Methods 10(3-4), 1984
PMID: 6530509
GTP-binding proteins in plants.
Bischoff F, Molendijk A, Rajendrakumar CS, Palme K., Cell. Mol. Life Sci. 55(2), 1999
PMID: 10188584
Bischoff F, Molendijk A, Rajendrakumar CS, Palme K., Cell. Mol. Life Sci. 55(2), 1999
PMID: 10188584
Characterization of the glyceraldehyde-3-phosphate dehydrogenase gene family from Kluyveromyces marxianus--polymerase chain reaction-single-strand conformation polymorphism as a tool for the study of multigenic families.
Fernandes PA, Sena-Esteves M, Moradas-Ferreira P., Yeast 11(8), 1995
PMID: 7668042
Fernandes PA, Sena-Esteves M, Moradas-Ferreira P., Yeast 11(8), 1995
PMID: 7668042
Nitric oxide functions as a signal in plant disease resistance.
Delledonne M, Xia Y, Dixon RA, Lamb C., Nature 394(6693), 1998
PMID: 9707120
Delledonne M, Xia Y, Dixon RA, Lamb C., Nature 394(6693), 1998
PMID: 9707120
GTP-binding proteins in plants: new members of an old family.
Ma H., Plant Mol. Biol. 26(5), 1994
PMID: 7858207
Ma H., Plant Mol. Biol. 26(5), 1994
PMID: 7858207
Transgenic tobacco plants that express an antisense construct derived from a Medicago sativa cDNA encoding a Rac-related small GTP-binding protein fail to develop necrotic lesions upon elicitor infiltration.
Schiene K, Puhler A, Niehaus K., Mol. Gen. Genet. 263(5), 2000
PMID: 10905344
Schiene K, Puhler A, Niehaus K., Mol. Gen. Genet. 263(5), 2000
PMID: 10905344
Rha1, an Arabidopsis Rab5 homolog, plays a critical role in the vacuolar trafficking of soluble cargo proteins.
Sohn EJ, Kim ES, Zhao M, Kim SJ, Kim H, Kim YW, Lee YJ, Hillmer S, Sohn U, Jiang L, Hwang I., Plant Cell 15(5), 2003
PMID: 12724533
Sohn EJ, Kim ES, Zhao M, Kim SJ, Kim H, Kim YW, Lee YJ, Hillmer S, Sohn U, Jiang L, Hwang I., Plant Cell 15(5), 2003
PMID: 12724533
The GTPase superfamily: conserved structure and molecular mechanism.
Bourne HR, Sanders DA, McCormick F., Nature 349(6305), 1991
PMID: 1898771
Bourne HR, Sanders DA, McCormick F., Nature 349(6305), 1991
PMID: 1898771
Analysis of the small GTPase gene superfamily of Arabidopsis.
Vernoud V, Horton AC, Yang Z, Nielsen E., Plant Physiol. 131(3), 2003
PMID: 12644670
Vernoud V, Horton AC, Yang Z, Nielsen E., Plant Physiol. 131(3), 2003
PMID: 12644670
Rab11, a small GTPase associated with both constitutive and regulated secretory pathways in PC12 cells.
Urbe S, Huber LA, Zerial M, Tooze SA, Parton RG., FEBS Lett. 334(2), 1993
PMID: 8224244
Urbe S, Huber LA, Zerial M, Tooze SA, Parton RG., FEBS Lett. 334(2), 1993
PMID: 8224244
Characterization and subcellular localization of a small GTP-binding protein (Ara-4) from Arabidopsis: conditional expression under control of the promoter of the gene for heat-shock protein HSP81-1.
Ueda T, Anai T, Tsukaya H, Hirata A, Uchimiya H., Mol. Gen. Genet. 250(5), 1996
PMID: 8676856
Ueda T, Anai T, Tsukaya H, Hirata A, Uchimiya H., Mol. Gen. Genet. 250(5), 1996
PMID: 8676856
Location of light-repressible, small GTP-binding protein of the YPT/rab family in the growing zone of etiolated pea stems.
Nagano Y, Okada Y, Narita H, Asaka Y, Sasaki Y., Proc. Natl. Acad. Sci. U.S.A. 92(14), 1995
PMID: 7603989
Nagano Y, Okada Y, Narita H, Asaka Y, Sasaki Y., Proc. Natl. Acad. Sci. U.S.A. 92(14), 1995
PMID: 7603989
Biogenesis of the peribacteroid membrane in root nodules.
Verma DP, Hong Z., Trends Microbiol. 4(9), 1996
PMID: 8885172
Verma DP, Hong Z., Trends Microbiol. 4(9), 1996
PMID: 8885172
A cDNA cloning method for monomeric GTP-binding proteins by ligand blotting.
Nagano Y, Matsuno R, Sasaki Y., Anal. Biochem. 211(2), 1993
PMID: 8317694
Nagano Y, Matsuno R, Sasaki Y., Anal. Biochem. 211(2), 1993
PMID: 8317694
Expression of the gene for a small GTP binding protein in transgenic tobacco elevates endogenous cytokinin levels, abnormally induces salicylic acid in response to wounding, and increases resistance to tobacco mosaic virus infection.
Sano H, Seo S, Orudgev E, Youssefian S, Ishizuka K., Proc. Natl. Acad. Sci. U.S.A. 91(22), 1994
PMID: 11607497
Sano H, Seo S, Orudgev E, Youssefian S, Ishizuka K., Proc. Natl. Acad. Sci. U.S.A. 91(22), 1994
PMID: 11607497
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
Thompson JD, Higgins DG, Gibson TJ., Nucleic Acids Res. 22(22), 1994
PMID: 7984417
Thompson JD, Higgins DG, Gibson TJ., Nucleic Acids Res. 22(22), 1994
PMID: 7984417
Localization of a Rho GTPase Implies a Role in Tip Growth and Movement of the Generative Cell in Pollen Tubes.
Lin Y, Wang Y, Zhu JK, Yang Z., Plant Cell 8(2), 1996
PMID: 12239385
Lin Y, Wang Y, Zhu JK, Yang Z., Plant Cell 8(2), 1996
PMID: 12239385
Developmental abnormalities and reduced fruit softening in tomato plants expressing an antisense Rab11 GTPase gene.
Lu C, Zainal Z, Tucker GA, Lycett GW., Plant Cell 13(8), 2001
PMID: 11487695
Lu C, Zainal Z, Tucker GA, Lycett GW., Plant Cell 13(8), 2001
PMID: 11487695
The diversity of Rab proteins in vesicle transport.
Novick P, Zerial M., Curr. Opin. Cell Biol. 9(4), 1997
PMID: 9261061
Novick P, Zerial M., Curr. Opin. Cell Biol. 9(4), 1997
PMID: 9261061
Export
Markieren/ Markierung löschen
Markierte Publikationen
Web of Science
Dieser Datensatz im Web of Science®Quellen
PMID: 15221459
PubMed | Europe PMC
Suchen in
Google Scholar