Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells

Seidel T, Kluge C, Hanitzsch M, Ross J, Sauer M, Dietz K-J, Golldack D (2004)
JOURNAL OF BIOTECHNOLOGY 112(1-2): 165-175.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The proton-translocating plant vacuolar H+-ATPase (VHA) is of prime importance for acidification of intracellular compartments and is essential for processes such as secondary activated transport, maintenance of ion homeostasis, and adaptation to environmental stress. Twelve genes have been identified that encode subunits of the functional V-ATPase complex. In this study, subunits c and a of the V-ATPase from the plant Mesembryanthemum crystallinum were fused to cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP), respectively, and were transiently coexpressed in protoplasts. Two-colour scanning confocal fluorescence microscopy demonstrates that the fusion proteins VHA-c-CFP and VHA-a-YFP are colocalized at the tonoplast, the plasmamembrane, and at endoplasmic membrane structures indicating expression in cytoplasmic vesicles. Furthermore, fluorescence resonance energy transfer (FRET) was used to visualize the interaction of VHA-c and VHA-a in vivo on the nanometer length scale. Excitation of CFP as donor fluorophore caused increased emission of YFP-fluorescence in protoplasts due to FRET. Our results give strong evidence for physical interaction of subunits c and a in living plant cells. (C) 2004 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
112
Ausgabe
1-2
Seite(n)
165-175
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PUB-ID

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Seidel T, Kluge C, Hanitzsch M, et al. Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells. JOURNAL OF BIOTECHNOLOGY. 2004;112(1-2):165-175.
Seidel, T., Kluge, C., Hanitzsch, M., Ross, J., Sauer, M., Dietz, K. - J., & Golldack, D. (2004). Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells. JOURNAL OF BIOTECHNOLOGY, 112(1-2), 165-175. doi:10.1016/j.jbiotec.2004.04.027
Seidel, T., Kluge, C., Hanitzsch, M., Ross, J., Sauer, M., Dietz, K. - J., and Golldack, D. (2004). Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells. JOURNAL OF BIOTECHNOLOGY 112, 165-175.
Seidel, T., et al., 2004. Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells. JOURNAL OF BIOTECHNOLOGY, 112(1-2), p 165-175.
T. Seidel, et al., “Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells”, JOURNAL OF BIOTECHNOLOGY, vol. 112, 2004, pp. 165-175.
Seidel, T., Kluge, C., Hanitzsch, M., Ross, J., Sauer, M., Dietz, K.-J., Golldack, D.: Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells. JOURNAL OF BIOTECHNOLOGY. 112, 165-175 (2004).
Seidel, Thorsten, Kluge, C, Hanitzsch, M, Ross, J, Sauer, Markus, Dietz, Karl-Josef, and Golldack, Dortje. “Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells”. JOURNAL OF BIOTECHNOLOGY 112.1-2 (2004): 165-175.

36 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Cytosolic GAPDH as a redox-dependent regulator of energy metabolism.
Schneider M, Knuesting J, Birkholz O, Heinisch JJ, Scheibe R., BMC Plant Biol 18(1), 2018
PMID: 30189844
Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176.
Mainali HR, Vadivel AK, Li X, Gijzen M, Dhaubhadel S., Sci Rep 7(), 2017
PMID: 28074922
Interacting protein partners of Arabidopsis RNA-binding protein AtRBP45b.
Muthuramalingam M, Wang Y, Li Y, Mahalingam R., Plant Biol (Stuttg) 19(3), 2017
PMID: 28039930
Heterogeneity in the expression and subcellular localization of POLYOL/MONOSACCHARIDE TRANSPORTER genes in Lotus japonicus.
Tian L, Liu L, Yin Y, Huang M, Chen Y, Xu X, Wu P, Li M, Wu G, Jiang H, Chen Y., PLoS One 12(9), 2017
PMID: 28931056
Function of glutathione peroxidases in legume root nodules.
Matamoros MA, Saiz A, Peñuelas M, Bustos-Sanmamed P, Mulet JM, Barja MV, Rouhier N, Moore M, James EK, Dietz KJ, Becana M., J Exp Bot 66(10), 2015
PMID: 25740929
The wheat E subunit of V-type H+-ATPase is involved in the plant response to osmotic stress.
Zhang XH, Li B, Hu YG, Chen L, Min DH., Int J Mol Sci 15(9), 2014
PMID: 25222556
Elements of transcriptional machinery are compatible among plants and mammals.
Wolf A, Akrap N, Marg B, Galliardt H, Heiligentag M, Humpert F, Sauer M, Kaltschmidt B, Kaltschmidt C, Seidel T., PLoS One 8(1), 2013
PMID: 23326494
Kaede for detection of protein oligomerization.
Wolf H, Barisas BG, Dietz KJ, Seidel T., Mol Plant 6(5), 2013
PMID: 23430050
Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells.
Müller SM, Galliardt H, Schneider J, Barisas BG, Seidel T., Front Plant Sci 4(), 2013
PMID: 24194740
Transfer of a Redox-Signal through the Cytosol by Redox-Dependent Microcompartmentation of Glycolytic Enzymes at Mitochondria and Actin Cytoskeleton.
Wojtera-Kwiczor J, Groß F, Leffers HM, Kang M, Schneider M, Scheibe R., Front Plant Sci 3(), 2012
PMID: 23316205
The CRYPTOCHROME1-dependent response to excess light is mediated through the transcriptional activators ZINC FINGER PROTEIN EXPRESSED IN INFLORESCENCE MERISTEM LIKE1 and ZML2 in Arabidopsis.
Shaikhali J, de Dios Barajas-Lopéz J, Ötvös K, Kremnev D, Garcia AS, Srivastava V, Wingsle G, Bako L, Strand Å., Plant Cell 24(7), 2012
PMID: 22786870
FdC1, a novel ferredoxin protein capable of alternative electron partitioning, increases in conditions of acceptor limitation at photosystem I.
Voss I, Goss T, Murozuka E, Altmann B, McLean KJ, Rigby SE, Munro AW, Scheibe R, Hase T, Hanke GT., J Biol Chem 286(1), 2011
PMID: 20966083
The cellular energization state affects peripheral stalk stability of plant vacuolar H+-ATPase and impairs vacuolar acidification.
Schnitzer D, Seidel T, Sander T, Golldack D, Dietz KJ., Plant Cell Physiol 52(5), 2011
PMID: 21474463
Multiple redox and non-redox interactions define 2-Cys peroxiredoxin as a regulatory hub in the chloroplast.
Muthuramalingam M, Seidel T, Laxa M, Nunes de Miranda SM, Gärtner F, Ströher E, Kandlbinder A, Dietz KJ., Mol Plant 2(6), 2009
PMID: 19995730
Regulation of plant cytosolic glyceraldehyde 3-phosphate dehydrogenase isoforms by thiol modifications.
Holtgrefe S, Gohlke J, Starmann J, Druce S, Klocke S, Altmann B, Wojtera J, Lindermayr C, Scheibe R., Physiol Plant 133(2), 2008
PMID: 18298409
The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes.
Shaikhali J, Heiber I, Seidel T, Ströher E, Hiltscher H, Birkmann S, Dietz KJ, Baier M., BMC Plant Biol 8(), 2008
PMID: 18439303
Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis.
Seidel T, Schnitzer D, Golldack D, Sauer M, Dietz KJ., BMC Cell Biol 9(), 2008
PMID: 18507826
Regulation of plant cytosolic glyceraldehyde 3-phosphate dehydrogenase isoforms by thiol modifications
Holtgrefe S, Gohlke J, Starmann J, Druce S, Klocke S, Altmann B, Wojtera J, Lindermayr C, Scheibe R., Physiol Plant 133(2), 2008
PMID: IND44051579
Knockout of major leaf ferredoxin reveals new redox-regulatory adaptations in Arabidopsis thaliana.
Voss I, Koelmann M, Wojtera J, Holtgrefe S, Kitzmann C, Backhausen JE, Scheibe R., Physiol Plant 133(3), 2008
PMID: 18494733
Knockout of major leaf ferredoxin reveals new redox-regulatory adaptations in Arabidopsis thaliana
Voss I, Koelmann M, Wojtera J, Holtgrefe S, Kitzmann C, Backhausen JE, Scheibe R., Physiol Plant 133(3), 2008
PMID: IND44069762
The redox imbalanced mutants of Arabidopsis differentiate signaling pathways for redox regulation of chloroplast antioxidant enzymes.
Heiber I, Ströher E, Raatz B, Busse I, Kahmann U, Bevan MW, Dietz KJ, Baier M., Plant Physiol 143(4), 2007
PMID: 17337533
Transcript level regulation of the vacuolar H(+)-ATPase subunit isoforms VHA-a, VHA-E and VHA-G in Arabidopsis thaliana.
Hanitzsch M, Schnitzer D, Seidel T, Golldack D, Dietz KJ., Mol Membr Biol 24(5-6), 2007
PMID: 17710654
Using intrinsically fluorescent proteins for plant cell imaging.
Dixit R, Cyr R, Gilroy S., Plant J 45(4), 2006
PMID: 16441351
Show and tell: cell biology of pathogen invasion.
Koh S, Somerville S., Curr Opin Plant Biol 9(4), 2006
PMID: 16714141
Members of the Arabidopsis AtTPK/KCO family form homomeric vacuolar channels in planta.
Voelker C, Schmidt D, Mueller-Roeber B, Czempinski K., Plant J 48(2), 2006
PMID: 16984403
Imaging protein molecules using FRET and FLIM microscopy.
Wallrabe H, Periasamy A., Curr Opin Biotechnol 16(1), 2005
PMID: 15722011
Mapping of C-termini of V-ATPase subunits by in vivo-FRET measurements.
Seidel T, Golldack D, Dietz KJ., FEBS Lett 579(20), 2005
PMID: 16061227

38 References

Daten bereitgestellt von Europe PubMed Central.

The photomorphogenesis regulator DET1 binds the amino-terminal tail of histone H2B in a nucleosome context.
Benvenuto G, Formiggini F, Laflamme P, Malakhov M, Bowler C., Curr. Biol. 12(17), 2002
PMID: 12225670

AUTHOR UNKNOWN, 0
Characterization of the epidermis from barley primary leaves : I. Isolation of epidermal protoplasts.
Dietz KJ, Schramm M, Betz M, Busch H, Durr C, Martinoia E., Planta 187(4), 1992
PMID: 24178135
Significance of the V-type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level.
Dietz KJ, Tavakoli N, Kluge C, Mimura T, Sharma SS, Harris GC, Chardonnens AN, Golldack D., J. Exp. Bot. 52(363), 2001
PMID: 11559732
Three-dimensional map of a plant V-ATPase based on electron microscopy.
Domgall I, Venzke D, Luttge U, Ratajczak R, Bottcher B., J. Biol. Chem. 277(15), 2002
PMID: 11815621
GFP-based FRET microscopy in living plant cells.
Gadella TW Jr, van der Krogt GN , Bisseling T., Trends Plant Sci. 4(7), 1999
PMID: 10407445
Transgenic plants as factories for biopharmaceuticals.
Giddings G, Allison G, Brooks D, Carter A., Nat. Biotechnol. 18(11), 2000
PMID: 11062432
Composition and assembly of the yeast vacuolar H(+)-ATPase complex.
Graham LA, Powell B, Stevens TH., J. Exp. Biol. 203(Pt 1), 2000
PMID: 10600674
Three-dimensional structure and subunit topology of the V(1) ATPase from Manduca sexta midgut.
Gruber G, Radermacher M, Ruiz T, Godovac-Zimmermann J, Canas B, Kleine-Kohlbrecher D, Huss M, Harvey WR, Wieczorek H., Biochemistry 39(29), 2000
PMID: 10913268
Vacuolar-Type H+ -ATPases Are Associated with the Endoplasmic Reticulum and Provacuoles of Root Tip Cells.
Herman EM, Li X, Su RT, Larsen P, Hsu H, Sze H., Plant Physiol. 106(4), 1994
PMID: 12232411
Expression of an insect (Dendroides canadensis) antifreeze protein in Arabidopsis thaliana results in a decrease in plant freezing temperature.
Huang T, Nicodemus J, Zarka DG, Thomashow MF, Wisniewski M, Duman JG., Plant Mol. Biol. 50(3), 2002
PMID: 12369611
Evidence for rotation of V1-ATPase.
Imamura H, Nakano M, Noji H, Muneyuki E, Ohkuma S, Yoshida M, Yokoyama K., Proc. Natl. Acad. Sci. U.S.A. 100(5), 2003
PMID: 12598655
Analysis of MADS box protein-protein interactions in living plant cells.
Immink RG, Gadella TW Jr, Ferrario S, Busscher M, Angenent GC., Proc. Natl. Acad. Sci. U.S.A. 99(4), 2002
PMID: 11854533
Expression of water channel proteins in Mesembryanthemum crystallinum.
Kirch HH, Vera-Estrella R, Golldack D, Quigley F, Michalowski CB, Barkla BJ, Bohnert HJ., Plant Physiol. 123(1), 2000
PMID: 10806230
cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress.
Kluge C, Lamkemeyer P, Tavakoli N, Golldack D, Kandlbinder A, Dietz KJ., Mol. Membr. Biol. 20(2), 2003
PMID: 12851073
New insight into the structure and regulation of the plant vacuolar H+-ATPase.
Kluge C, Lahr J, Hanitzsch M, Bolte S, Golldack D, Dietz KJ., J. Bioenerg. Biomembr. 35(4), 2003
PMID: 14635783
Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants.
Kovtun Y, Chiu WL, Tena G, Sheen J., Proc. Natl. Acad. Sci. U.S.A. 97(6), 2000
PMID: 10717008
STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p.
Manolson MF, Wu B, Proteau D, Taillon BE, Roberts BT, Hoyt MA, Jones EW., J. Biol. Chem. 269(19), 1994
PMID: 7514599
Characterization of yeast V-ATPase mutants lacking Vph1p or Stv1p and the effect on endocytosis.
Perzov N, Padler-Karavani V, Nelson H, Nelson N., J. Exp. Biol. 205(Pt 9), 2002
PMID: 11948198
Structure, function and regulation of the plant vacuolar H(+)-translocating ATPase.
Ratajczak R., Biochim. Biophys. Acta 1465(1-2), 2000
PMID: 10748245
Immunological detection of tonoplast polypeptides in the plasma membrane of pea cotyledons
Robinson, Planta 198(), 1996
The renaissance of fluorescence resonance energy transfer.
Selvin PR., Nat. Struct. Biol. 7(9), 2000
PMID: 10966639
The Arabidopsis kinase-associated protein phosphatase controls internalization of the somatic embryogenesis receptor kinase 1.
Shah K, Russinova E, Gadella TW Jr, Willemse J, De Vries SC., Genes Dev. 16(13), 2002
PMID: 12101128
A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase.
Sze H, Schumacher K, Muller ML, Padmanaban S, Taiz L., Trends Plant Sci. 7(4), 2002
PMID: 11950611
The green fluorescent protein.
Tsien RY., Annu. Rev. Biochem. 67(), 1998
PMID: 9759496
Plant virus expression systems for transient production of recombinant allergens in Nicotiana benthamiana.
Wagner B, Fuchs H, Adhami F, Ma Y, Scheiner O, Breiteneder H., Methods 32(3), 2004
PMID: 14962756
Fluorescence spectroscopy of single biomolecules.
Weiss S., Science 283(5408), 1999
PMID: 10073925
The ATP-waiting conformation of rotating F1-ATPase revealed by single-pair fluorescence resonance energy transfer.
Yasuda R, Masaike T, Adachi K, Noji H, Itoh H, Kinosita K Jr., Proc. Natl. Acad. Sci. U.S.A. 100(16), 2003
PMID: 12876203
Rotation of the proteolipid ring in the V-ATPase.
Yokoyama K, Nakano M, Imamura H, Yoshida M, Tamakoshi M., J. Biol. Chem. 278(27), 2003
PMID: 12707282

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