PUB - Publikationen an der Universität Bielefeld

The non-toxic compound N-acetyl-L-phosphinothricin (N-Ac-L-PPT) is used in a so-called deacetylation system to induce male sterility in transgenic plants by tapetum specific deacetylation to the herbicide L-phosphinothricin (L-PPT). A procedure was developed to produce pure racemic and L-isomeric N-Ac-PPT containing less than 30 ppm residual PPT. Experiments applied to wild type tobacco and PPT-resistant tobacco showed that the maximal tolerated N-Ac-PPT concentration would be less than 45 mM of the L-isomer. Otherwise unspecific deacetylation by several acylases, as well as by environmental conditions like higher temperatures or pHs beyond neutrality, increased the residual L-PPT content to toxic concentrations. In contrast, N-acetyl-L-phosphinothricyl-alanyl-alanine (N-Ac-L-PPTT), a substance also occurring during the biosynthesis of phosphinothricyl-alanyl-alanine (PPTT) by some Streptomyces species, was tolerated up to 274 mM by wild type tobacco plants. However, the ArgE deacatylase from Escherichia coli originally used in the deacetylation system, as well as some other acylases, showed no activity towards N-Ac-L-PPTT.