Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells

Braun SG, Meyer A, Olst O, Pühler A, Niehaus K (2005)
Molecular Plant - Microbe Interactions 18(7): 674-681.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The lipopolysaccharides (LPS) of gram-negative bacteria are essential for perception of pathogens by animals and plants. To identify the LPS substructure or substructures recognized by plants, we isolated water-phase (w)LPS from different Xanthomonas campestris pv. campestris mutants and analyzed their sugar content and ability to elicit an oxidative burst in tobacco cell cultures. The different wLPS species are characterized by lacking repetitive subunits of the O-antigen, the complete O-antigen, or even most of the core region. Because loss of lipid A would be lethal to bacteria, pure lipid A was obtained from X. campestris pv. campestris wild-type wLPS by chemical hydrolysis. The elicitation experiments with tobacco cell cultures revealed that LPS detection is dependent on the bioavailability of the amphiphilic wLPS, which can form micelles in an aqueous environment. By adding deoxycholate to prevent micelle formation, all of the tested wLPS species showed elicitation capability, whereas the lipid A alone was not able to trigger an oxidative burst or calcium transients in tobacco cell cultures. These results suggest that the LPS substructure recognized by tobacco cells is localized in the inner core region of the LPS, consisting of glucose, gallacturonic acid, and 3-deoxy-d-manno-oct-2-ulosonic acids. Although lipid A alone seems to be insufficient to induce an oxidative burst in tobacco cell cultures, it cannot be ruled out that lipid A or the glucosamine backbone may be important in combination with the inner core structures.
Stichworte
pathogen-associated molecular patterns (PAMPs); nonhost resistance
Erscheinungsjahr
2005
Zeitschriftentitel
Molecular Plant - Microbe Interactions
Band
18
Ausgabe
7
Seite(n)
674-681
ISSN
0894-0282
Page URI
https://pub.uni-bielefeld.de/record/1603291

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Braun SG, Meyer A, Olst O, Pühler A, Niehaus K. Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells. Molecular Plant - Microbe Interactions. 2005;18(7):674-681.
Braun, S. G., Meyer, A., Olst, O., Pühler, A., & Niehaus, K. (2005). Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells. Molecular Plant - Microbe Interactions, 18(7), 674-681. doi:10.1094/MPMI-18-0674
Braun, S. G., Meyer, A., Olst, O., Pühler, A., and Niehaus, K. (2005). Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells. Molecular Plant - Microbe Interactions 18, 674-681.
Braun, S.G., et al., 2005. Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells. Molecular Plant - Microbe Interactions, 18(7), p 674-681.
S.G. Braun, et al., “Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells”, Molecular Plant - Microbe Interactions, vol. 18, 2005, pp. 674-681.
Braun, S.G., Meyer, A., Olst, O., Pühler, A., Niehaus, K.: Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells. Molecular Plant - Microbe Interactions. 18, 674-681 (2005).
Braun, S. G., Meyer, A., Olst, O., Pühler, Alfred, and Niehaus, Karsten. “Characterization of the Xanthomonas campestris pv. campestris lipopolysaccharide substructures essential for elicitation of an oxidative burst in tobacco cells”. Molecular Plant - Microbe Interactions 18.7 (2005): 674-681.

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