The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase

Larisch C, Nakunst D, Hueser AT, Tauch A, Kalinowski J (2007)
BMC Genomics 8(1): 4.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
Larisch, Christof; Nakunst, Diana; Hueser, Andrea T.; Tauch, AndreasUniBi; Kalinowski, JörnUniBi
Abstract / Bemerkung
Background: Corynebacterium glutamicum is a gram-positive soil bacterium widely used for the industrial production of amino acids. There is great interest in the examination of the molecular mechanism of transcription control. One of these control mechanisms are sigma factors. C. glutamicum ATCC 13032 has seven putative sigma factor-encoding genes, including sigA and sigB. The sigA gene encodes the essential primary sigma factor of C. glutamicum and is responsible for promoter recognition of house-keeping genes. The sigB gene codes for the non-essential sigma factor SigB that has a proposed role in stress reponse. Results: The sigB gene expression was highest at transition between exponential growth and stationary phase, when the amount of sigA mRNA was already decreasing. Genome-wide transcription profiles of the wild-type and the sigB mutant were recorded by comparative DNA microarray hybridizations. The data indicated that the mRNA levels of 111 genes are significantly changed in the sigB-proficient strain during the transition phase, whereas the expression profile of the sigB-deficient strain showed only minor changes ( 26 genes). The genes that are higher expressed during transition phase only in the sigB-proficient strain mainly belong to the functional categories amino acid metabolism, carbon metabolism, stress defense, membrane processes, and phosphorus metabolism. The transcription start points of six of these genes were determined and the deduced promoter sequences turned out to be indistinguishable from that of the consensus promoter recognized by SigA. Real-time reverse transcription PCR assays revealed that the expression profiles of these genes during growth were similar to that of the sigB gene itself. In the sigB mutant, however, the transcription profiles resembled that of the sigA gene encoding the house-keeping sigma factor. Conclusion: During transition phase, the sigB gene showed an enhanced expression, while simultaneously the sigA mRNA decreased in abundance. This might cause a replacement of SigA by SigB at the RNA polymerase core enzyme and in turn results in increased expression of genes relevant for the transition and the stationary phase, either to cope with nutrient limitation or with the accompanying oxidative stress. The increased expression of genes encoding anti-oxidative or protection functions also prepares the cell for upcoming limitations and environmental stresses.
BMC Genomics
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Larisch C, Nakunst D, Hueser AT, Tauch A, Kalinowski J. The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase. BMC Genomics. 2007;8(1): 4.
Larisch, C., Nakunst, D., Hueser, A. T., Tauch, A., & Kalinowski, J. (2007). The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase. BMC Genomics, 8(1), 4.
Larisch, C., Nakunst, D., Hueser, A. T., Tauch, A., and Kalinowski, J. (2007). The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase. BMC Genomics 8:4.
Larisch, C., et al., 2007. The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase. BMC Genomics, 8(1): 4.
C. Larisch, et al., “The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase”, BMC Genomics, vol. 8, 2007, : 4.
Larisch, C., Nakunst, D., Hueser, A.T., Tauch, A., Kalinowski, J.: The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase. BMC Genomics. 8, : 4 (2007).
Larisch, Christof, Nakunst, Diana, Hueser, Andrea T., Tauch, Andreas, and Kalinowski, Jörn. “The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase”. BMC Genomics 8.1 (2007): 4.
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36 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Identifying the Growth Modulon of Corynebacterium glutamicum.
Haas T, Graf M, Nieß A, Busche T, Kalinowski J, Blombach B, Takors R., Front Microbiol 10(), 2019
PMID: 31134020
Global transcriptomic analysis of the response of Corynebacterium glutamicum to ferulic acid.
Chen C, Pan J, Yang X, Xiao H, Zhang Y, Si M, Shen X, Wang Y., Arch Microbiol 199(2), 2017
PMID: 27766354
Enhancement of Microbial Biodesulfurization via Genetic Engineering and Adaptive Evolution.
Wang J, Butler RR, Wu F, Pombert JF, Kilbane JJ, Stark BC., PLoS One 12(1), 2017
PMID: 28060828
Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum.
Dostálová H, Holátko J, Busche T, Rucká L, Rapoport A, Halada P, Nešvera J, Kalinowski J, Pátek M., AMB Express 7(1), 2017
PMID: 28651382
Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors.
Šilar R, Holátko J, Rucká L, Rapoport A, Dostálová H, Kadeřábková P, Nešvera J, Pátek M., Curr Microbiol 73(3), 2016
PMID: 27270733
The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response.
Pahlke J, Dostálová H, Holátko J, Degner U, Bott M, Pátek M, Polen T., RNA Biol 13(9), 2016
PMID: 27362471
Global Transcriptomic Analysis of the Response of Corynebacterium glutamicum to Vanillin.
Chen C, Pan J, Yang X, Guo C, Ding W, Si M, Zhang Y, Shen X, Wang Y., PLoS One 11(10), 2016
PMID: 27760214
Influence of SigB inactivation on Corynebacterium glutamicum protein secretion.
Watanabe K, Teramoto H, Suzuki N, Inui M, Yukawa H., Appl Microbiol Biotechnol 97(11), 2013
PMID: 23179627
Two-dimensional fluorescence difference gel electrophoresis analysis of Listeria monocytogenes submitted to a redox shock.
Ignatova M, Guével B, Com E, Haddad N, Rossero A, Bogard P, Prévost H, Guillou S., J Proteomics 79(), 2013
PMID: 23195917
Corynebacterium glutamicum promoters: a practical approach.
Pátek M, Holátko J, Busche T, Kalinowski J, Nešvera J., Microb Biotechnol 6(2), 2013
PMID: 23305350
Construction of in vitro transcription system for Corynebacterium glutamicum and its use in the recognition of promoters of different classes.
Holátko J, Silar R, Rabatinová A, Sanderová H, Halada P, Nešvera J, Krásný L, Pátek M., Appl Microbiol Biotechnol 96(2), 2012
PMID: 22885668
Identification of the membrane protein SucE and its role in succinate transport in Corynebacterium glutamicum.
Huhn S, Jolkver E, Krämer R, Marin K., Appl Microbiol Biotechnol 89(2), 2011
PMID: 20809072
Autoinduction of a genetic locus encoding putative acyltransferase in Corynebacterium glutamicum.
Shin HS, Kim YJ, Yoo IH, Lee HS, Jin S, Ha UH., Biotechnol Lett 33(1), 2011
PMID: 20821248
Tools for genetic manipulations in Corynebacterium glutamicum and their applications.
Nešvera J, Pátek M., Appl Microbiol Biotechnol 90(5), 2011
PMID: 21519933
Growth and reducing capacity of Listeria monocytogenes under different initial redox potential.
Ignatova M, Prévost H, Leguerinel I, Guillou S., J Appl Microbiol 108(1), 2010
PMID: 19645768
Factors enhancing L-valine production by the growth-limited L-isoleucine auxotrophic strain Corynebacterium glutamicum DeltailvA DeltapanB ilvNM13 (pECKAilvBNC).
Denina I, Paegle L, Prouza M, Holátko J, Pátek M, Nesvera J, Ruklisha M., J Ind Microbiol Biotechnol 37(7), 2010
PMID: 20364396
Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum.
Barreiro C, Nakunst D, Hüser AT, de Paz HD, Kalinowski J, Martín JF., Microbiology 155(pt 2), 2009
PMID: 19202085
A game with many players: control of gdh transcription in Corynebacterium glutamicum.
Hänssler E, Müller T, Palumbo K, Patek M, Brocker M, Krämer R, Burkovski A., J Biotechnol 142(2), 2009
PMID: 19394370
Gene expression analysis of Corynebacterium glutamicum subjected to long-term lactic acid adaptation.
Jakob K, Satorhelyi P, Lange C, Wendisch VF, Silakowski B, Scherer S, Neuhaus K., J Bacteriol 189(15), 2007
PMID: 17526706

49 References

Daten bereitgestellt von Europe PubMed Central.

Bacterial RNA polymerase.
Darst SA., Curr. Opin. Struct. Biol. 11(2), 2001
PMID: 11297923
Structure and function of bacterial sigma factors.
Helmann JD, Chamberlin MJ., Annu. Rev. Biochem. 57(), 1988
PMID: 3052291
The sigma 70 family: sequence conservation and evolutionary relationships.
Lonetto M, Gribskov M, Gross CA., J. Bacteriol. 174(12), 1992
PMID: 1597408
A consensus structure for sigma S-dependent promoters.
Espinosa-Urgel M, Chamizo C, Tormo A., Mol. Microbiol. 21(3), 1996
PMID: 8866487
Compilation of E. coli mRNA promoter sequences.
Lisser S, Margalit H., Nucleic Acids Res. 21(7), 1993
PMID: 8479900
Genomic organization of the mycobacterial sigma gene cluster.
Doukhan L, Predich M, Nair G, Dussurget O, Mandic-Mulec I, Cole ST, Smith DR, Smith I., Gene 165(1), 1995
PMID: 7489918
Global analysis of the general stress response of Bacillus subtilis.
Petersohn A, Brigulla M, Haas S, Hoheisel JD, Volker U, Hecker M., J. Bacteriol. 183(19), 2001
PMID: 11544224
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Kramer R, Linke B, McHardy AC, Meyer F, Mockel B, Pfefferle W, Puhler A, Rey DA, Ruckert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948626
Multiple sigma factor genes in Brevibacterium lactofermentum: characterization of sigA and sigB.
Oguiza JA, Marcos AT, Malumbres M, Martin JF., J. Bacteriol. 178(2), 1996
PMID: 8550480
Cloning and transcriptional characterization of two sigma factor genes, sigA and sigB, from Brevibacterium flavum.
Halgasova N, Bukovska G, Timko J, Kormanec J., Curr. Microbiol. 43(4), 2001
PMID: 11683358
Promoters of Corynebacterium glutamicum.
Patek M, Nesvera J, Guyonvarch A, Reyes O, Leblon G., J. Biotechnol. 104(1-3), 2003
PMID: 12948648
Transcriptional analysis of the sigA and sigB genes of Brevibacterium lactofermentum.
Oguiza JA, Marcos AT, Martin JF., FEMS Microbiol. Lett. 153(1), 1997
PMID: 9252580
The Brevibacterium flavum sigma factor SigB has a role in the environmental stress response.
Halgasova N, Bukovska G, Ugorcakova J, Timko J, Kormanec J., FEMS Microbiol. Lett. 216(1), 2002
PMID: 12423756
Eubacterial sigma-factors.
Wosten MM., FEMS Microbiol. Rev. 22(3), 1998
PMID: 9818380
Development of a Corynebacterium glutamicum DNA microarray and validation by genome-wide expression profiling during growth with propionate as carbon source.
Huser AT, Becker A, Brune I, Dondrup M, Kalinowski J, Plassmeier J, Puhler A, Wiegrabe I, Tauch A., J. Biotechnol. 106(2-3), 2003
PMID: 14651867
EMMA: a platform for consistent storage and efficient analysis of microarray data.
Dondrup M, Goesmann A, Bartels D, Kalinowski J, Krause L, Linke B, Rupp O, Sczyrba A, Puhler A, Meyer F., J. Biotechnol. 106(2-3), 2003
PMID: 14651856
Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bioB gene from Brevibacterium flavum.
Hatakeyama K, Kohama K, Vertes AA, Kobayashi M, Kurusu Y, Yukawa H., DNA Seq. 4(2), 1993
PMID: 8173080
Role of the ssu and seu genes of Corynebacterium glutamicum ATCC 13032 in utilization of sulfonates and sulfonate esters as sulfur sources.
Koch DJ, Ruckert C, Rey DA, Mix A, Puhler A, Kalinowski J., Appl. Environ. Microbiol. 71(10), 2005
PMID: 16204527
The transcriptional regulator SsuR activates expression of the Corynebacterium glutamicum sulphonate utilization genes in the absence of sulphate.
Koch DJ, Ruckert C, Albersmeier A, Huser AT, Tauch A, Puhler A, Kalinowski J., Mol. Microbiol. 58(2), 2005
PMID: 16194234
Transcriptional analysis of the gap-pgk-tpi-ppc gene cluster of Corynebacterium glutamicum.
Schwinde JW, Thum-Schmitz N, Eikmanns BJ, Sahm H., J. Bacteriol. 175(12), 1993
PMID: 7685337
Identification of an anion-specific channel in the cell wall of the Gram-positive bacterium Corynebacterium glutamicum.
Costa-Riu N, Maier E, Burkovski A, Kramer R, Lottspeich F, Benz R., Mol. Microbiol. 50(4), 2003
PMID: 14622416
Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response.
Kocan M, Schaffer S, Ishige T, Sorger-Herrmann U, Wendisch VF, Bott M., J. Bacteriol. 188(2), 2006
PMID: 16385062
The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses.
Ishige T, Krause M, Bott M, Wendisch VF, Sahm H., J. Bacteriol. 185(15), 2003
PMID: 12867461
Identification of a growth phase-dependent promoter in the rplJL operon of Streptomyces coelicolor A3(2).
Blanco G, Sanchez C, Rodicio MR, Mendez C, Salas JA., Biochim. Biophys. Acta 1517(2), 2001
PMID: 11342105
Biosynthesis of pantothenic acid and coenzyme A
Jackowski S., 1996
Identification of conserved, RpoS-dependent stationary-phase genes of Escherichia coli.
Schellhorn HE, Audia JP, Wei LI, Chang L., J. Bacteriol. 180(23), 1998
PMID: 9829938
A master regulator sigmaB governs osmotic and oxidative response as well as differentiation via a network of sigma factors in Streptomyces coelicolor.
Lee EJ, Karoonuthaisiri N, Kim HS, Park JH, Cha CJ, Kao CM, Roe JH., Mol. Microbiol. 57(5), 2005
PMID: 16101999
Negative regulation by RpoS: a case of sigma factor competition.
Farewell A, Kvint K, Nystrom T., Mol. Microbiol. 29(4), 1998
PMID: 9767572
Role of the general stress response during strong overexpression of a heterologous gene in Escherichia coli.
Schweder T, Lin HY, Jurgen B, Breitenstein A, Riemschneider S, Khalameyzer V, Gupta A, Buttner K, Neubauer P., Appl. Microbiol. Biotechnol. 58(3), 2002
PMID: 11935184

Sambrook J., 1989
Protoplast transformation of glutamate-producing bacteria with plasmid DNA.
Katsumata R, Ozaki A, Oka T, Furuya A., J. Bacteriol. 159(1), 1984
PMID: 6145700
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
Grant SG, Jessee J, Bloom FR, Hanahan D., Proc. Natl. Acad. Sci. U.S.A. 87(12), 1990
PMID: 2162051
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668


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