Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage

Kubitzki T, Noll T, Luetz S (2008)
BIOPROCESS AND BIOSYSTEMS ENGINEERING 31(3): 173-182.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Kubitzki, Tina; Noll, ThomasUniBi ; Luetz, Stephan
Abstract / Bemerkung
Two immobilisation methods for enterokinase were developed, which yielded high remaining activities for the cleavage of the fusion protein MUC1-IgG Fc. Different carrier materials were compared regarding remaining enzyme activity and storage stability. Immobilisation procedures involving support material activation using glutardialdehyde were found to result in low remaining activities. Applying less aggressive activation procedures, remaining activities of similar to 60% were received when immobilising enterokinase on either Estapor paramagnetic microspheres or hexamethylamino Sepabeads(R). In case of hexamethylamino Sepabeads(R) we were able to increase the half-life time 4.3-fold at 23 degrees C and 3.8-fold at 4 degrees C compared to the free enzyme at the same temperatures. By immobilising the biocatalyst the downstream process is simplified allowing the easy removal of the enzyme from the reaction mixture. The immobilised enterokinase cleaves the fusion protein MUC1-IgG Fc in at least two repeated batches, proving the efficiency of the immobilisation method and the reusability of the biocatalyst.
Stichworte
immobilisation; fusion protein cleavage; enterokinase
Erscheinungsjahr
2008
Zeitschriftentitel
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Band
31
Ausgabe
3
Seite(n)
173-182
ISSN
1615-7591
eISSN
1615-7605
Page URI
https://pub.uni-bielefeld.de/record/1592236

Zitieren

Kubitzki T, Noll T, Luetz S. Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2008;31(3):173-182.
Kubitzki, T., Noll, T., & Luetz, S. (2008). Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 31(3), 173-182. https://doi.org/10.1007/s00449-007-0191-3
Kubitzki, Tina, Noll, Thomas, and Luetz, Stephan. 2008. “Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 31 (3): 173-182.
Kubitzki, T., Noll, T., and Luetz, S. (2008). Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING 31, 173-182.
Kubitzki, T., Noll, T., & Luetz, S., 2008. Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 31(3), p 173-182.
T. Kubitzki, T. Noll, and S. Luetz, “Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 31, 2008, pp. 173-182.
Kubitzki, T., Noll, T., Luetz, S.: Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 31, 173-182 (2008).
Kubitzki, Tina, Noll, Thomas, and Luetz, Stephan. “Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 31.3 (2008): 173-182.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Engineering botulinum neurotoxin domains for activation by toxin light chain.
Stancombe PR, Masuyer G, Birch-Machin I, Beard M, Foster KA, Chaddock JA, Acharya KR., FEBS J 279(3), 2012
PMID: 22141396
Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins.
Miladi B, El Marjou A, Boeuf G, Bouallagui H, Dufour F, Di Martino P, Elm'selmi A., J Biotechnol 158(3), 2012
PMID: 22300512
Immobilization of enterokinase on magnetic supports for the cleavage of fusion proteins.
Santana SD, Pina AS, Roque AC., J Biotechnol 161(3), 2012
PMID: 22820339
Immobilization and stabilization of α-galactosidase on Sepabeads EC-EA and EC-HA.
Bayraktar H, Serilmez M, Karkaş T, Celem EB, Onal S., Int J Biol Macromol 49(4), 2011
PMID: 21871914
High level expression of human enteropeptidase light chain in Pichia pastoris.
Pepeliaev S, Krahulec J, Černý Z, Jílková J, Tlustá M, Dostálová J., J Biotechnol 156(1), 2011
PMID: 21884736
Application of immobilized bovine enterokinase in repetitive fusion protein cleavage for the production of mucin 1.
Kubitzki T, Minör D, Mackfeld U, Oldiges M, Noll T, Lütz S., Biotechnol J 4(11), 2009
PMID: 19670252

40 References

Daten bereitgestellt von Europe PubMed Central.

PURIFICATION AND CONCENTRATION OF ENTEROKINASE.
Kunitz M., J. Gen. Physiol. 22(4), 1939
PMID: 19873113

S, Comp Biochem Physiol 42(), 1972

LA, Biotechnology 13(), 1995

ER, Biotechnology 11(), 1993

TP, Biotechnology 6(), 1988
Horse cytokine/IgG fusion proteins--mammalian expression of biologically active cytokines and a system to verify antibody specificity to equine cytokines.
Wagner B, Robeson J, McCracken M, Wattrang E, Antczak DF., Vet. Immunol. Immunopathol. 105(1-2), 2005
PMID: 15797470

N, Food Technol Biotechnol 41(), 2003
An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein.
Maina CV, Riggs PD, Grandea AG 3rd, Slatko BE, Moran LS, Tagliamonte JA, McReynolds LA, Guan CD., Gene 74(2), 1988
PMID: 3073105
Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme.
Martinez A, Knappskog PM, Olafsdottir S, Doskeland AP, Eiken HG, Svebak RM, Bozzini M, Apold J, Flatmark T., Biochem. J. 306 ( Pt 2)(), 1995
PMID: 7887915
Enzymic cleavage of fusion protein using immobilized urokinase covalently conjugated to glyoxyl-agarose.
Suh CW, Choi GS, Lee EK., Biotechnol. Appl. Biochem. 37(Pt 2), 2003
PMID: 12630903
An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I.
Forsberg G, Baastrup B, Rondahl H, Holmgren E, Pohl G, Hartmanis M, Lake M., J. Protein Chem. 11(2), 1992
PMID: 1388667
Recombinant MUC1 mucin with a breast cancer-like O-glycosylation produced in large amounts in Chinese-hamster ovary cells.
Backstrom M, Link T, Olson FJ, Karlsson H, Graham R, Picco G, Burchell J, Taylor-Papadimitriou J, Noll T, Hansson GC., Biochem. J. 376(Pt 3), 2003
PMID: 12950230

S, Angew Chem Int Ed 44(), 2005

S, Angew Chem Int Ed 44(), 2005
Expression and functional characterization of recombinant human HDAC1 and HDAC3.
Li J, Staver MJ, Curtin ML, Holms JH, Frey RR, Edalji R, Smith R, Michaelides MR, Davidsen SK, Glaser KB., Life Sci. 74(22), 2004
PMID: 15043985

S, Chem Eng Technol 29(), 2006
Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites.
Mateo C, Abian O, Fernandez-Lafuente R, Guisan JM., Biotechnol. Bioeng. 68(1), 2000
PMID: 10699877

F, Tetrahedron Asymmetry 17(), 2006
Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs).
Schoevaart R, Wolbers MW, Golubovic M, Ottens M, Kieboom AP, van Rantwijk F, van der Wielen LA, Sheldon RA., Biotechnol. Bioeng. 87(6), 2004
PMID: 15329933
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
Bastida A, Sabuquillo P, Armisen P, Fernandez-Lafuente R, Huguet J, Guisan JM., Biotechnol. Bioeng. 58(5), 1998
PMID: 10099284

RA, Biocatal Biotransformation 23(), 2005

N, Tetrahedron Asymmetry 15(), 2004

AC, Biocatal Biotransformation 22(), 2004

L, Tetrahedron 60(), 2004

DM, Enzyme Microb Technol 36(), 2005
A novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: immobilization-stabilization of beta-galactosidase from Aspergillus oryzae.
Torres R, Mateo C, Fernandez-Lorente G, Ortiz C, Fuentes M, Palomo JM, Guisan JM, Fernandez-Lafuente R., Biotechnol. Prog. 19(3), 2003
PMID: 12790680

M, Biotechnol Lett 23(), 2001
The preparation and properties of bovine enterokinase.
Liepnieks JJ, Light A., J. Biol. Chem. 254(5), 1979
PMID: 762166
Optimization of an industrial biocatalyst of glutaryl acylase: stabilization of the enzyme by multipoint covalent attachment onto new amino-epoxy Sepabeads.
Lopez-Gallego F, Betancor L, Hidalgo A, Mateo C, Guisan JM, Fernandez-Lafuente R., J. Biotechnol. 111(2), 2004
PMID: 15219407
Phase I trial of a murine antibody to MUC1 in patients with metastatic cancer: evidence for the activation of humoral and cellular antitumor immunity.
de Bono JS, Rha SY, Stephenson J, Schultes BC, Monroe P, Eckhardt GS, Hammond LA, Whiteside TL, Nicodemus CF, Cermak JM, Rowinsky EK, Tolcher AW., Ann. Oncol. 15(12), 2004
PMID: 15550589
Intramuscular immunisation with MUC1 cDNA can protect C57 mice challenged with MUC1-expressing syngeneic mouse tumour cells.
Graham RA, Burchell JM, Beverley P, Taylor-Papadimitriou J., Int. J. Cancer 65(5), 1996
PMID: 8598319

CW, Process Biochem 40(), 2005

SJ, Biotechnol Bioprocess Eng 7(), 2002
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 18183425
PubMed | Europe PMC

Suchen in

Google Scholar