Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage

Kubitzki T, Noll T, Luetz S (2008)
BIOPROCESS AND BIOSYSTEMS ENGINEERING 31(3): 173-182.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Two immobilisation methods for enterokinase were developed, which yielded high remaining activities for the cleavage of the fusion protein MUC1-IgG Fc. Different carrier materials were compared regarding remaining enzyme activity and storage stability. Immobilisation procedures involving support material activation using glutardialdehyde were found to result in low remaining activities. Applying less aggressive activation procedures, remaining activities of similar to 60% were received when immobilising enterokinase on either Estapor paramagnetic microspheres or hexamethylamino Sepabeads(R). In case of hexamethylamino Sepabeads(R) we were able to increase the half-life time 4.3-fold at 23 degrees C and 3.8-fold at 4 degrees C compared to the free enzyme at the same temperatures. By immobilising the biocatalyst the downstream process is simplified allowing the easy removal of the enzyme from the reaction mixture. The immobilised enterokinase cleaves the fusion protein MUC1-IgG Fc in at least two repeated batches, proving the efficiency of the immobilisation method and the reusability of the biocatalyst.
Stichworte
immobilisation; fusion protein cleavage; enterokinase
Erscheinungsjahr
2008
Zeitschriftentitel
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Band
31
Ausgabe
3
Seite(n)
173-182
ISSN
1615-7591
eISSN
1615-7605
Page URI
https://pub.uni-bielefeld.de/record/1592236

Zitieren

Kubitzki T, Noll T, Luetz S. Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2008;31(3):173-182.
Kubitzki, T., Noll, T., & Luetz, S. (2008). Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 31(3), 173-182. doi:10.1007/s00449-007-0191-3
Kubitzki, T., Noll, T., and Luetz, S. (2008). Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING 31, 173-182.
Kubitzki, T., Noll, T., & Luetz, S., 2008. Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 31(3), p 173-182.
T. Kubitzki, T. Noll, and S. Luetz, “Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 31, 2008, pp. 173-182.
Kubitzki, T., Noll, T., Luetz, S.: Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 31, 173-182 (2008).
Kubitzki, Tina, Noll, Thomas, and Luetz, Stephan. “Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage”. BIOPROCESS AND BIOSYSTEMS ENGINEERING 31.3 (2008): 173-182.

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