Substrate Walking for Selective Ene Reduction
Brasselet H, Berger J, Arends E, de Man A, van der Weel L, van Dijk H, Hummel A, Gröger H, Hanefeld U (2026)
ChemCatChem 18(9): e70753.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Brasselet, Hugo;
Berger, Jonathan;
Arends, Elisa;
de Man, Adriaan;
van der Weel, Laura;
van Dijk, Hessel;
Hummel, AnkeUniBi;
Gröger, HaraldUniBi;
Hanefeld, Ulf
Abstract / Bemerkung
Ene-reductases (EREDs) efficiently reduce activated C & boxH;C bonds, but their activity toward tetra-substituted alkenes has been largely unexplored. Here, we report the first systematic study demonstrating that several EREDs catalyze the stereoselective reduction of tri- and tetra-substituted cyclohexenones while investigating the influence of different cofactors. To enable a scalable application, the best enzyme (YqjM) was co-immobilized with glucose dehydrogenase on Ni-NTA cellulose beads, achieving >99% immobilization efficiency and high operational stability. The immobilized system was successfully scaled to 200 mL, reaching full substrate conversion without enzyme degradation over 9 days.
Erscheinungsjahr
2026
Zeitschriftentitel
ChemCatChem
Band
18
Ausgabe
9
Art.-Nr.
e70753
ISSN
1867-3880
eISSN
1867-3899
Page URI
https://pub.uni-bielefeld.de/record/3016975
Zitieren
Brasselet H, Berger J, Arends E, et al. Substrate Walking for Selective Ene Reduction. ChemCatChem . 2026;18(9): e70753.
Brasselet, H., Berger, J., Arends, E., de Man, A., van der Weel, L., van Dijk, H., Hummel, A., et al. (2026). Substrate Walking for Selective Ene Reduction. ChemCatChem , 18(9), e70753. https://doi.org/10.1002/cctc.70753
Brasselet, Hugo, Berger, Jonathan, Arends, Elisa, de Man, Adriaan, van der Weel, Laura, van Dijk, Hessel, Hummel, Anke, Gröger, Harald, and Hanefeld, Ulf. 2026. “Substrate Walking for Selective Ene Reduction”. ChemCatChem 18 (9): e70753.
Brasselet, H., Berger, J., Arends, E., de Man, A., van der Weel, L., van Dijk, H., Hummel, A., Gröger, H., and Hanefeld, U. (2026). Substrate Walking for Selective Ene Reduction. ChemCatChem 18:e70753.
Brasselet, H., et al., 2026. Substrate Walking for Selective Ene Reduction. ChemCatChem , 18(9): e70753.
H. Brasselet, et al., “Substrate Walking for Selective Ene Reduction”, ChemCatChem , vol. 18, 2026, : e70753.
Brasselet, H., Berger, J., Arends, E., de Man, A., van der Weel, L., van Dijk, H., Hummel, A., Gröger, H., Hanefeld, U.: Substrate Walking for Selective Ene Reduction. ChemCatChem . 18, : e70753 (2026).
Brasselet, Hugo, Berger, Jonathan, Arends, Elisa, de Man, Adriaan, van der Weel, Laura, van Dijk, Hessel, Hummel, Anke, Gröger, Harald, and Hanefeld, Ulf. “Substrate Walking for Selective Ene Reduction”. ChemCatChem 18.9 (2026): e70753.
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