PUB - Publikationen an der Universität Bielefeld

**Abstract**
Deleterious mutations are ubiquitous in natural populations and, when expressed, reduce fitness. However, the specific nature of these mutations and the mechanisms through which they impact fitness remain poorly understood. We leveraged recent advances in genomics to predict deleterious mutations in the black grouse, an iconic lekking species. Analysis of 190 whole genomes alongside comprehensive life history data including repeated measures of behavioural, ornamental and fitness traits, revealed that deleterious mutations identified through evolutionary conservation and functional prediction are strongly associated with reduced male lifetime mating success. Both homozygous and heterozygous mutations reduce fitness, indicating that fully and partially recessive mutations contribute towards an individual’s realized mutation load. Notably, deleterious mutations in promotors have disproportionally negative fitness effects, suggesting they impair an individual’s ability to dynamically adjust gene expression to meet context-dependent functional demands. Finally, deleterious mutations impact male mating success by reducing lek attendance rather than by altering the expression of ornamental traits, implying that behaviour serves as an honest indicator of genetic quality. These findings offer novel insights into the genetic architecture of sexually selected traits and illuminate the complex interplay between genetic variation and phenotypic expression, with important implications for evolutionary biology and conservation genomics.