The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells
Kotschnew K, Winkler D, Reckmann J, Mann C, Schweigert A, Tellkamp G, Müller K, Fischer von Mollard G (2024)
European Journal of Cell Biology 103(4): 151461.
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Abstract / Bemerkung
During intracellular trafficking N-ethylmaleimide-sensitive-factor attachment receptor (SNARE) proteins catalyze the membrane fusion by assembling into a four-helix complex. In the mouse model, loss of the endosomal SNAREs vti1a and vti1b results in a perinatal lethal phenotype and neuronal defects including decreased neurite outgrowth in cultured primary neurons. We used a CRISPR/Cas9 system to generate a Vti1a Vti1b double knockout (DKO) in the neuroblastoma cell line N1E-115. Three different DKO cell lines were obtained and examined at genome and protein level. The double deficiency impaired proper differentiation based on lower levels of synaptic proteins as well as reduced neurite formation and elongation compared to wild type cells in differentiation medium. Neurite elongation can be induced by a variety of extracellular signals via different signaling cascades. Treatment with the Rho kinase inhibitor Y27632, which stimulates enlargeosome exocytosis, or with neurotrophic factors (BDNF, NGF and NT3) resulted in reduced stimulation of all DKO clones and in significantly shorter neurites compared to wild type cells. The loss of vti1a and vti1b disrupted Akt signaling during enlargeosome-mediated and Erk signaling during BDNF-induced neurite outgrowth. Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.
Erscheinungsjahr
2024
Zeitschriftentitel
European Journal of Cell Biology
Band
103
Ausgabe
4
Art.-Nr.
151461
eISSN
1618-1298
Page URI
https://pub.uni-bielefeld.de/record/2993656
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Kotschnew K, Winkler D, Reckmann J, et al. The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells. European Journal of Cell Biology. 2024;103(4): 151461.
Kotschnew, K., Winkler, D., Reckmann, J., Mann, C., Schweigert, A., Tellkamp, G., Müller, K., et al. (2024). The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells. European Journal of Cell Biology, 103(4), 151461. https://doi.org/10.1016/j.ejcb.2024.151461
Kotschnew, Katharina, Winkler, Denise, Reckmann, Jonas, Mann, Charlotte, Schweigert, Alina, Tellkamp, Greta, Müller, Kristian, and Fischer von Mollard, Gabriele. 2024. “The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells”. European Journal of Cell Biology 103 (4): 151461.
Kotschnew, K., Winkler, D., Reckmann, J., Mann, C., Schweigert, A., Tellkamp, G., Müller, K., and Fischer von Mollard, G. (2024). The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells. European Journal of Cell Biology 103:151461.
Kotschnew, K., et al., 2024. The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells. European Journal of Cell Biology, 103(4): 151461.
K. Kotschnew, et al., “The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells”, European Journal of Cell Biology, vol. 103, 2024, : 151461.
Kotschnew, K., Winkler, D., Reckmann, J., Mann, C., Schweigert, A., Tellkamp, G., Müller, K., Fischer von Mollard, G.: The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells. European Journal of Cell Biology. 103, : 151461 (2024).
Kotschnew, Katharina, Winkler, Denise, Reckmann, Jonas, Mann, Charlotte, Schweigert, Alina, Tellkamp, Greta, Müller, Kristian, and Fischer von Mollard, Gabriele. “The double deficiency of the SNARE proteins vti1a and vti1b affects neurite outgrowth and signaling in N1E-115 neuroblastoma cells”. European Journal of Cell Biology 103.4 (2024): 151461.
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