High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy
Ortkraß H, Müller M, Engdahl AK, Holst G, Huser T (2024)
Nature Communications 15(1): 8886.
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Autor*in
Ortkraß, HenningUniBi;
Müller, MarcelUniBi ;
Engdahl, Anders KokkvollUniBi;
Holst, Gerhard;
Huser, ThomasUniBi
Einrichtung
Abstract / Bemerkung
High-resolution optical fluorescence microscopies and, in particular, super-resolution fluorescence microscopy, are rapidly adopting highly sensitive cameras as their preferred photodetectors. Camera-based parallel detection facilitates high-speed live cell imaging with the highest spatial resolution. Here, we show that the drive to use ever more sensitive, photon-counting image sensors in cameras can, however, have detrimental effects on the spatial resolution of the resulting images. This is particularly noticeable in applications that demand a high space-bandwidth product, where the image magnification is close to the Nyquist sampling limit of the sensor. Most scientists will often select image sensors based on parameters such as pixel size, quantum efficiency, signal-to-noise performance, dynamic range, and frame rate of the sensor. A parameter that is, however, typically overlooked is the sensor's modulation transfer function (MTF). We have determined the wavelength-specific MTF of front- and back-illuminated image sensors and evaluated how it affects the spatial resolution that can be achieved in high-resolution fluorescence microscopy modalities. We find significant differences in image sensor performance that cause the resulting spatial resolution to vary by up to 28%. This result shows that the choice of image sensor has a significant impact on the imaging performance of all camera-based optical microscopy modalities. © 2024. The Author(s).
Erscheinungsjahr
2024
Zeitschriftentitel
Nature Communications
Band
15
Ausgabe
1
Art.-Nr.
8886
ISSN
2041-1723
Page URI
https://pub.uni-bielefeld.de/record/2993651
Zitieren
Ortkraß H, Müller M, Engdahl AK, Holst G, Huser T. High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy. Nature Communications . 2024;15(1): 8886.
Ortkraß, H., Müller, M., Engdahl, A. K., Holst, G., & Huser, T. (2024). High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy. Nature Communications , 15(1), 8886. https://doi.org/10.1038/s41467-024-53198-1
Ortkraß, Henning, Müller, Marcel, Engdahl, Anders Kokkvoll, Holst, Gerhard, and Huser, Thomas. 2024. “High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy”. Nature Communications 15 (1): 8886.
Ortkraß, H., Müller, M., Engdahl, A. K., Holst, G., and Huser, T. (2024). High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy. Nature Communications 15:8886.
Ortkraß, H., et al., 2024. High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy. Nature Communications , 15(1): 8886.
H. Ortkraß, et al., “High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy”, Nature Communications , vol. 15, 2024, : 8886.
Ortkraß, H., Müller, M., Engdahl, A.K., Holst, G., Huser, T.: High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy. Nature Communications . 15, : 8886 (2024).
Ortkraß, Henning, Müller, Marcel, Engdahl, Anders Kokkvoll, Holst, Gerhard, and Huser, Thomas. “High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy”. Nature Communications 15.1 (2024): 8886.
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