Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation
Gendreizig S, Martínez-Ruiz L, López-Rodríguez A, Pabla H, Hose L, Brasch F, Busche T, Escames G, Sudhoff H, Scholtz L-U, Todt I, et al. (2024)
Cell Death & Disease 15(7): 517.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Gendreizig, SarahUniBi ;
Martínez-Ruiz, Laura;
López-Rodríguez, Alba;
Pabla, Harkiren;
Hose, LeonieUniBi ;
Brasch, Frank;
Busche, TobiasUniBi;
Escames, Germaine;
Sudhoff, HolgerUniBi ;
Scholtz, Lars-Uwe;
Todt, IngoUniBi;
Oppel, FelixUniBi
Alle
Alle
Einrichtung
Abstract / Bemerkung
Head and neck squamous cell carcinoma (HNSCC) is a highly malignant disease, and death rates have remained at approximately 50% for decades. New tumor-targeting strategies are desperately needed, and a previous report indicated the triggered differentiation of HPV-negative HNSCC cells to confer therapeutic benefits. Using patient-derived tumor cells, we created a similar HNSCC differentiation model of HPV+ tumor cells from two patients. We observed a loss of malignant characteristics in differentiating cell culture conditions, including irregularly enlarged cell morphology, cell cycle arrest with downregulation of Ki67, and reduced cell viability. RNA-Seq showed myocyte-like differentiation with upregulation of markers of myofibril assembly. Immunofluorescence staining of differentiated and undifferentiated primary HPV+HNSCC cells confirmed an upregulation of these markers and the formation of parallel actin fibers reminiscent of myoblast-lineage cells. Moreover, immunofluorescence of HPV+ tumor tissue revealed areas of cells co-expressing the identified markers of myofibril assembly, HPV surrogate marker p16, and stress-associated basal keratinocyte marker KRT17, indicating that the observed myocyte-like in vitro differentiation occurs in human tissue. We are the first to report that carcinoma cells can undergo a triggered myocyte-like differentiation, and our study suggests that the targeted differentiation of HPV+ HNSCCs might be therapeutically valuable. © 2024. The Author(s).
Erscheinungsjahr
2024
Zeitschriftentitel
Cell Death & Disease
Band
15
Ausgabe
7
Art.-Nr.
517
eISSN
2041-4889
Page URI
https://pub.uni-bielefeld.de/record/2991561
Zitieren
Gendreizig S, Martínez-Ruiz L, López-Rodríguez A, et al. Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation. Cell Death & Disease . 2024;15(7): 517.
Gendreizig, S., Martínez-Ruiz, L., López-Rodríguez, A., Pabla, H., Hose, L., Brasch, F., Busche, T., et al. (2024). Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation. Cell Death & Disease , 15(7), 517. https://doi.org/10.1038/s41419-024-06867-4
Gendreizig, Sarah, Martínez-Ruiz, Laura, López-Rodríguez, Alba, Pabla, Harkiren, Hose, Leonie, Brasch, Frank, Busche, Tobias, et al. 2024. “Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation”. Cell Death & Disease 15 (7): 517.
Gendreizig, S., Martínez-Ruiz, L., López-Rodríguez, A., Pabla, H., Hose, L., Brasch, F., Busche, T., Escames, G., Sudhoff, H., Scholtz, L. - U., et al. (2024). Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation. Cell Death & Disease 15:517.
Gendreizig, S., et al., 2024. Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation. Cell Death & Disease , 15(7): 517.
S. Gendreizig, et al., “Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation”, Cell Death & Disease , vol. 15, 2024, : 517.
Gendreizig, S., Martínez-Ruiz, L., López-Rodríguez, A., Pabla, H., Hose, L., Brasch, F., Busche, T., Escames, G., Sudhoff, H., Scholtz, L.-U., Todt, I., Oppel, F.: Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation. Cell Death & Disease . 15, : 517 (2024).
Gendreizig, Sarah, Martínez-Ruiz, Laura, López-Rodríguez, Alba, Pabla, Harkiren, Hose, Leonie, Brasch, Frank, Busche, Tobias, Escames, Germaine, Sudhoff, Holger, Scholtz, Lars-Uwe, Todt, Ingo, and Oppel, Felix. “Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation”. Cell Death & Disease 15.7 (2024): 517.
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