Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics
Heyer R, Schallert K, Briza M, Benndorf D (2024)
Methods in Molecular Biology 2820: 99–113.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Heyer, RobertUniBi;
Schallert, Kay;
Briza, Marie;
Benndorf, Dirk
Abstract / Bemerkung
Metaproteomics represents a promising and fast method to analyze the taxonomic and functional composition of biogas plant microbiomes. However, metaproteomics sample preparation and bioinformatics analysis is still challenging due to the sample complexity and contaminants. In this chapter, a tailored workflow including sampling, phenol extraction in a ball mill, amido black protein quantification, FASP digestion, LC-MS/MS measurement as well as bioinformatics and biostatistical data evaluation are here described for the metaproteomics advancements applied to biogas plant samples. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Erscheinungsjahr
2024
Zeitschriftentitel
Methods in Molecular Biology
Band
2820
Seite(n)
99–113
eISSN
1940-6029
Page URI
https://pub.uni-bielefeld.de/record/2990930
Zitieren
Heyer R, Schallert K, Briza M, Benndorf D. Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics. Methods in Molecular Biology . 2024;2820:99–113.
Heyer, R., Schallert, K., Briza, M., & Benndorf, D. (2024). Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics. Methods in Molecular Biology , 2820, 99–113. https://doi.org/10.1007/978-1-0716-3910-8_10
Heyer, Robert, Schallert, Kay, Briza, Marie, and Benndorf, Dirk. 2024. “Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics”. Methods in Molecular Biology 2820: 99–113.
Heyer, R., Schallert, K., Briza, M., and Benndorf, D. (2024). Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics. Methods in Molecular Biology 2820, 99–113.
Heyer, R., et al., 2024. Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics. Methods in Molecular Biology , 2820, p 99–113.
R. Heyer, et al., “Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics”, Methods in Molecular Biology , vol. 2820, 2024, pp. 99–113.
Heyer, R., Schallert, K., Briza, M., Benndorf, D.: Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics. Methods in Molecular Biology . 2820, 99–113 (2024).
Heyer, Robert, Schallert, Kay, Briza, Marie, and Benndorf, Dirk. “Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics”. Methods in Molecular Biology 2820 (2024): 99–113.
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