Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution
Bartos LM, Kirchleitner SV, Kolabas ZI, Quach S, Beck A, Lorenz J, Blobner J, Mueller SA, Ulukaya S, Hoeher L, Horvath I, et al. (2023)
Science Advances 9(43).
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Bartos, Laura M.;
Kirchleitner, Sabrina V.;
Kolabas, Zeynep Ilgin;
Quach, StefanieUniBi 
;
Beck, Alexander;
Lorenz, Julia;
Blobner, Jens;
Mueller, Stephan A.;
Ulukaya, Selin;
Hoeher, Luciano;
Horvath, Izabela;
Wind-Mark, Karin
Alle
;
Beck, Alexander;
Lorenz, Julia;
Blobner, Jens;
Mueller, Stephan A.;
Ulukaya, Selin;
Hoeher, Luciano;
Horvath, Izabela;
Wind-Mark, Karin
Alle
Abstract / Bemerkung
Various cellular sources hamper interpretation of positron emission tomography (PET) biomarkers in the tumor microenvironment (TME). We developed an approach of immunomagnetic cell sorting after in vivo radiotracer injection (scRadiotracing) with three-dimensional (3D) histology to dissect the cellular allocation of PET signals in the TME. In mice with implanted glioblastoma, translocator protein (TSPO) radiotracer uptake per tumor cell was higher compared to tumor-associated microglia/macrophages (TAMs), validated by protein levels. Translation of in vitro scRadiotracing to patients with glioma immediately after tumor resection confirmed higher single-cell TSPO tracer uptake of tumor cells compared to immune cells. Across species, cellular radiotracer uptake explained the heterogeneity of individual TSPO-PET signals. In consideration of cellular tracer uptake and cell type abundance, tumor cells were the main contributor to TSPO enrichment in glioblastoma; however, proteomics identified potential PET targets highly specific for TAMs. Combining cellular tracer uptake measures with 3D histology facilitates precise allocation of PET signals and serves to validate emerging novel TAM-specific radioligands.
The assessment of single-cell tracer uptake and 3D histology advances development of TAM-specific PET biomarkers in glioblastoma.
The assessment of single-cell tracer uptake and 3D histology advances development of TAM-specific PET biomarkers in glioblastoma.
Erscheinungsjahr
2023
Zeitschriftentitel
Science Advances
Band
9
Ausgabe
43
eISSN
2375-2548
Page URI
https://pub.uni-bielefeld.de/record/2988833
Zitieren
Bartos LM, Kirchleitner SV, Kolabas ZI, et al. Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution. Science Advances. 2023;9(43).
Bartos, L. M., Kirchleitner, S. V., Kolabas, Z. I., Quach, S., Beck, A., Lorenz, J., Blobner, J., et al. (2023). Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution. Science Advances, 9(43). https://doi.org/10.1126/sciadv.adi8986
Bartos, Laura M., Kirchleitner, Sabrina V., Kolabas, Zeynep Ilgin, Quach, Stefanie, Beck, Alexander, Lorenz, Julia, Blobner, Jens, et al. 2023. “Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution”. Science Advances 9 (43).
Bartos, L. M., Kirchleitner, S. V., Kolabas, Z. I., Quach, S., Beck, A., Lorenz, J., Blobner, J., Mueller, S. A., Ulukaya, S., Hoeher, L., et al. (2023). Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution. Science Advances 9.
Bartos, L.M., et al., 2023. Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution. Science Advances, 9(43).
L.M. Bartos, et al., “Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution”, Science Advances, vol. 9, 2023.
Bartos, L.M., Kirchleitner, S.V., Kolabas, Z.I., Quach, S., Beck, A., Lorenz, J., Blobner, J., Mueller, S.A., Ulukaya, S., Hoeher, L., Horvath, I., Wind-Mark, K., Holzgreve, A., Ruf, V.C., Gold, L., Kunze, L.H., Kunte, S.T., Beumers, P., Park, H.E., Antons, M., Zatcepin, A., Briel, N., Hoermann, L., Schaefer, R., Messerer, D., Bartenstein, P., Riemenschneider, M.J., Lindner, S., Ziegler, S., Herms, J., Lichtenthaler, S.F., Ertürk, A., Tonn, J.C., von Baumgarten, L., Albert, N.L., Brendel, M.: Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution. Science Advances. 9, (2023).
Bartos, Laura M., Kirchleitner, Sabrina V., Kolabas, Zeynep Ilgin, Quach, Stefanie, Beck, Alexander, Lorenz, Julia, Blobner, Jens, Mueller, Stephan A., Ulukaya, Selin, Hoeher, Luciano, Horvath, Izabela, Wind-Mark, Karin, Holzgreve, Adrien, Ruf, Viktoria C., Gold, Lukas, Kunze, Lea H., Kunte, Sebastian T., Beumers, Philipp, Park, Ha Eun, Antons, Melissa, Zatcepin, Artem, Briel, Nils, Hoermann, Leonie, Schaefer, Rebecca, Messerer, Denise, Bartenstein, Peter, Riemenschneider, Markus J., Lindner, Simon, Ziegler, Sibylle, Herms, Jochen, Lichtenthaler, Stefan F., Ertürk, Ali, Tonn, Joerg C., von Baumgarten, Louisa, Albert, Nathalie L., and Brendel, Matthias. “Deciphering sources of PET signals in the tumor microenvironment of glioblastoma at cellular resolution”. Science Advances 9.43 (2023).
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Preprint: 10.1101/2023.01.26.522174
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