Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle
Frei E, Huster M, Smital P, Schlossmann J, Hofmann F, Wegener J (2009)
American Journal of Physiology-Gastrointestinal and Liver Physiology 297(4): G834-G839.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Frei, Eva;
Huster, Maria;
Smital, Petra;
Schlossmann, Jens;
Hofmann, Franz;
Wegener, JörgUniBi ![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Abstract / Bemerkung
cGMP-dependent protein kinase I (cGKI) induces relaxation of smooth muscle via several pathways that include inhibition of intracellular Ca2+signaling and/or involve activation of myosin phosphatase. In the present study, we investigated these mechanisms comparatively in colon and jejunum longitudinal smooth muscle from mice. In simultaneous recordings from colon muscle, 8-bromo-cGMP (8-Br-cGMP) reduced both carbachol-induced tension and carbachol-induced increase in intracellular Ca2+concentration ([Ca2+]i). These effects of 8-Br-cGMP were absent in colon from mice carrying a mutated inositol-1,4,5 trisphosphate receptor I-associated G kinase substrate (IRAG) gene or lacking cGKI. However, in jejunum, 8-Br-cGMP reduced carbachol-induced tension but did not change corresponding [Ca2+]isignals. This setting was also observed in jejunum from mice carrying a mutated IRAG gene, whereas no response to 8-Br-cGMP was observed in jejunum from mice lacking cGKI. After inhibition of phosphatase activity by calyculin A, 8-Br-cGMP did not relax jejunum but still relaxed colon muscle. In Western blot analysis, 8-Br-cGMP reduced the signal for phosphorylated MYPT-1 in carbachol-stimulated jejunum but not in colon. These results suggest that cGMP/cGKI signaling differentially inhibits contraction in the muscles investigated: in jejunum, inhibition is performed without changing [Ca2+]iand is dependent on phosphatase activity, whereas in colon, inhibition is mediated by inhibition of [Ca2+]isignals.
Erscheinungsjahr
2009
Zeitschriftentitel
American Journal of Physiology-Gastrointestinal and Liver Physiology
Band
297
Ausgabe
4
Seite(n)
G834-G839
ISSN
0193-1857
eISSN
1522-1547
Page URI
https://pub.uni-bielefeld.de/record/2985980
Zitieren
Frei E, Huster M, Smital P, Schlossmann J, Hofmann F, Wegener J. Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2009;297(4):G834-G839.
Frei, E., Huster, M., Smital, P., Schlossmann, J., Hofmann, F., & Wegener, J. (2009). Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle. American Journal of Physiology-Gastrointestinal and Liver Physiology, 297(4), G834-G839. https://doi.org/10.1152/ajpgi.00095.2009
Frei, Eva, Huster, Maria, Smital, Petra, Schlossmann, Jens, Hofmann, Franz, and Wegener, Jörg. 2009. “Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle”. American Journal of Physiology-Gastrointestinal and Liver Physiology 297 (4): G834-G839.
Frei, E., Huster, M., Smital, P., Schlossmann, J., Hofmann, F., and Wegener, J. (2009). Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle. American Journal of Physiology-Gastrointestinal and Liver Physiology 297, G834-G839.
Frei, E., et al., 2009. Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle. American Journal of Physiology-Gastrointestinal and Liver Physiology, 297(4), p G834-G839.
E. Frei, et al., “Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle”, American Journal of Physiology-Gastrointestinal and Liver Physiology, vol. 297, 2009, pp. G834-G839.
Frei, E., Huster, M., Smital, P., Schlossmann, J., Hofmann, F., Wegener, J.: Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle. American Journal of Physiology-Gastrointestinal and Liver Physiology. 297, G834-G839 (2009).
Frei, Eva, Huster, Maria, Smital, Petra, Schlossmann, Jens, Hofmann, Franz, and Wegener, Jörg. “Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle”. American Journal of Physiology-Gastrointestinal and Liver Physiology 297.4 (2009): G834-G839.