Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes
Wegener J, Nawrath H (1995)
Naunyn-Schmiedeberg's Archives of Pharmacology 352(3).
Zeitschriftenaufsatz
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Autor*in
Wegener, JörgUniBi
;
Nawrath, Hermann
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Abstract / Bemerkung
The effects of the phenylalkylamines verapamil, gallopamil, and devapamil on L-type calcium currents (ICa) were studied in ventricular myocytes from rat hearts using the whole-cell patch-clamp technique. In particular, the question was addressed, whether the pharmacological binding sites for these drugs were located at the inner and/or at the outer surface of the cell membrane. Therefore, tertiary verapamil, gallopamil, and devapamil and their corresponding quaternary derivatives were applied either from the outside or the inside of the cell membrane. Extracellular application of verapamil, gallopamil and devapamil (each at 3 μM) reduced Ica to 16.1 ±8.6%, 11 ± 8.9 %, and 9.3 ± 6 % of control, respectively. Intracellular application of the same substances, via the patch pipette filled with 30 μM of either verapamil, gallopamil, or devapamil, failed to depress ICa. The quaternary derivatives of the phenylalkylamines (30 μM) were ineffective both when applied extracellularly or intracellularly. It is suggested that phenylalkylamines block ICa in ventricular myocytes by acting on a binding site of the calcium channel molecule located at the outer surface of the cell membrane.
Erscheinungsjahr
1995
Zeitschriftentitel
Naunyn-Schmiedeberg's Archives of Pharmacology
Band
352
Ausgabe
3
ISSN
0028-1298
eISSN
1432-1912
Page URI
https://pub.uni-bielefeld.de/record/2984515
Zitieren
Wegener J, Nawrath H. Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes. Naunyn-Schmiedeberg's Archives of Pharmacology. 1995;352(3).
Wegener, J., & Nawrath, H. (1995). Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes. Naunyn-Schmiedeberg's Archives of Pharmacology, 352(3). https://doi.org/10.1007/BF00168564
Wegener, Jörg, and Nawrath, Hermann. 1995. “Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes”. Naunyn-Schmiedeberg's Archives of Pharmacology 352 (3).
Wegener, J., and Nawrath, H. (1995). Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes. Naunyn-Schmiedeberg's Archives of Pharmacology 352.
Wegener, J., & Nawrath, H., 1995. Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes. Naunyn-Schmiedeberg's Archives of Pharmacology, 352(3).
J. Wegener and H. Nawrath, “Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes”, Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 352, 1995.
Wegener, J., Nawrath, H.: Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes. Naunyn-Schmiedeberg's Archives of Pharmacology. 352, (1995).
Wegener, Jörg, and Nawrath, Hermann. “Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes”. Naunyn-Schmiedeberg's Archives of Pharmacology 352.3 (1995).