Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle
Ertl C, Lukowski R, Sigl K, Schlossmann J, Hofmann F, Wegener J (2011)
European Journal of Pharmacology 670(1): 266-271.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Ertl, Claudia;
Lukowski, Robert;
Sigl, Katja;
Schlossmann, Jens;
Hofmann, Franz;
Wegener, JörgUniBi
Abstract / Bemerkung
cGMP-dependent kinase I (cGKI) is a major mediator of smooth muscle relaxation and exists in two isoforms, α and β. Both isoforms are supposed to mediate their effects via different intracellular signaling pathways. To verify this concept, the kinetics of relaxation mediated by either isoform was analyzed in gastric fundus smooth muscle from mice. Muscles from mice that express selectively the Iα or Iβ isoform of cGKI in smooth muscle (sm-cGKIα or sm-cGKIβ mice) were compared to muscles from conventional cGKI−/− mice. Fundus muscles were contracted by carbachol and then relaxed by 8-Br-cGMP or by electrical field stimulation (EFS). The time course of relaxation by 8-Br-cGMP was not different between muscles from sm-cGKIα and sm-cGKIβ mice. EFS induced a fast transient relaxation in muscles from sm-cGKIα and sm-cGKIβ mice that was blocked by the NO synthase inhibitor L-NAME. Recovery from this relaxation was about 4-times slower in muscles from sm-cGKIα mice than in muscles from sm-cGKIβ mice. The different kinetic of recovery from relaxation after EFS in sm-cGKIα and sm-cGKIβ mice suggests that different signaling pathways exist for each cGKI isoform in vivo in fundus muscles.
Erscheinungsjahr
2011
Zeitschriftentitel
European Journal of Pharmacology
Band
670
Ausgabe
1
Seite(n)
266-271
ISSN
00142999
Page URI
https://pub.uni-bielefeld.de/record/2984485
Zitieren
Ertl C, Lukowski R, Sigl K, Schlossmann J, Hofmann F, Wegener J. Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle. European Journal of Pharmacology. 2011;670(1):266-271.
Ertl, C., Lukowski, R., Sigl, K., Schlossmann, J., Hofmann, F., & Wegener, J. (2011). Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle. European Journal of Pharmacology, 670(1), 266-271. https://doi.org/10.1016/j.ejphar.2011.07.048
Ertl, Claudia, Lukowski, Robert, Sigl, Katja, Schlossmann, Jens, Hofmann, Franz, and Wegener, Jörg. 2011. “Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle”. European Journal of Pharmacology 670 (1): 266-271.
Ertl, C., Lukowski, R., Sigl, K., Schlossmann, J., Hofmann, F., and Wegener, J. (2011). Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle. European Journal of Pharmacology 670, 266-271.
Ertl, C., et al., 2011. Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle. European Journal of Pharmacology, 670(1), p 266-271.
C. Ertl, et al., “Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle”, European Journal of Pharmacology, vol. 670, 2011, pp. 266-271.
Ertl, C., Lukowski, R., Sigl, K., Schlossmann, J., Hofmann, F., Wegener, J.: Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle. European Journal of Pharmacology. 670, 266-271 (2011).
Ertl, Claudia, Lukowski, Robert, Sigl, Katja, Schlossmann, Jens, Hofmann, Franz, and Wegener, Jörg. “Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle”. European Journal of Pharmacology 670.1 (2011): 266-271.