L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function
Hofmann F, Flockerzi V, Kahl S, Wegener J (2014)
Physiological Reviews 94(1): 303-326.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Hofmann, Franz;
Flockerzi, Veit;
Kahl, Sabine;
Wegener, JörgUniBi ![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Abstract / Bemerkung
The L-type Cav1.2 calcium channel is present throughout the animal kingdom and is essential for some aspects of CNS function, cardiac and smooth muscle contractility, neuroendocrine regulation, and multiple other processes. The L-type CaV1.2 channel is built by up to four subunits; all subunits exist in various splice variants that potentially affect the biophysical and biological functions of the channel. Many of the CaV1.2 channel properties have been analyzed in heterologous expression systems including regulation of the L-type CaV1.2 channel by Ca2+itself and protein kinases. However, targeted mutations of the calcium channel genes confirmed only some of these in vitro findings. Substitution of the respective serines by alanine showed that β-adrenergic upregulation of the cardiac CaV1.2 channel did not depend on the phosphorylation of the in vitro specified amino acids. Moreover, well-established in vitro phosphorylation sites of the CaVβ2subunit of the cardiac L-type CaV1.2 channel were found to be irrelevant for the in vivo regulation of the channel. However, the molecular basis of some kinetic properties, such as Ca2+-dependent inactivation and facilitation, has been approved by in vivo mutagenesis of the CaV1.2α1gene. This article summarizes recent findings on the in vivo relevance of well-established in vitro results.
Erscheinungsjahr
2014
Zeitschriftentitel
Physiological Reviews
Band
94
Ausgabe
1
Seite(n)
303-326
ISSN
0031-9333
eISSN
1522-1210
Page URI
https://pub.uni-bielefeld.de/record/2984477
Zitieren
Hofmann F, Flockerzi V, Kahl S, Wegener J. L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function. Physiological Reviews. 2014;94(1):303-326.
Hofmann, F., Flockerzi, V., Kahl, S., & Wegener, J. (2014). L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function. Physiological Reviews, 94(1), 303-326. https://doi.org/10.1152/physrev.00016.2013
Hofmann, Franz, Flockerzi, Veit, Kahl, Sabine, and Wegener, Jörg. 2014. “L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function”. Physiological Reviews 94 (1): 303-326.
Hofmann, F., Flockerzi, V., Kahl, S., and Wegener, J. (2014). L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function. Physiological Reviews 94, 303-326.
Hofmann, F., et al., 2014. L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function. Physiological Reviews, 94(1), p 303-326.
F. Hofmann, et al., “L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function”, Physiological Reviews, vol. 94, 2014, pp. 303-326.
Hofmann, F., Flockerzi, V., Kahl, S., Wegener, J.: L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function. Physiological Reviews. 94, 303-326 (2014).
Hofmann, Franz, Flockerzi, Veit, Kahl, Sabine, and Wegener, Jörg. “L-Type Ca 1.2 Calcium Channels: From In Vitro Findings to In Vivo Function”. Physiological Reviews 94.1 (2014): 303-326.