Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser

Kong C, Pilger C, Kunisch M, Förster C, Schulte am Esch J, Huser T (2023)
Laser & Photonics Reviews 17(12): 9.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Abstract / Bemerkung
Coherent Raman scattering (CRS) microscopy is an attractive label-free imaging method providing high chemical sensitivity, sub-micron spatial resolution, and video-rate imaging speed. When extended to hyperspectral CRS (HS-CRS), it is capable of distinguishing molecules even in complex heterogeneous systems by probing multiple Raman resonances. Solid-state lasers, associated with their high cost, large footprint, and slow tuning speed, currently limit the widespread application of HS-CRS. Fiber lasers with comparable properties usually suffer from poor noise performance or instabilities during wavelength tuning. A tunable two-color fiber laser is demonstrated that can be electronically tuned at rates of up to 1 kHz. The use of polarization-maintaining fibers and an all-optical synchronization mechanism guarantees its mode-locking stability. The superior noise performance of this system is evaluated by the side-by-side comparison with data obtained by a commercial optical parametric oscillator (OPO)-based laser. Large-area multimodal imaging of liver tissue sections surgically resected from a patient suffering from a non-neoplastic liver parenchyma with micronodular cirrhosis in combination with a multilocular hepatocellular carcinoma is demonstrated. By exploiting the fast-hyperspectral scanning capability of the laser and multivariate statistical analysis, virtual staining of tissue sections and a comparison of the diagnostic properties of HS-CRS to the classical histopathological approach are achieved. Hyperspectral Coherent Raman Scattering microscopy is capable of distinguishing molecules in complex heterogeneous systems by probing multiple Raman resonances. A rapidly tunable two-color fiber laser is demonstrated. The superior noise performance of this system is evaluated by the side-by-side comparison with data from a commercial optical parametric oscillator-based laser. Large-area multimodal imaging of human liver tissue sections is demonstrated.image
Stichworte
coherent Raman scattering; multivariate statistical analysis; Raman; microscopy; Raman scattering; stimulated Raman scattering
Erscheinungsjahr
2023
Zeitschriftentitel
Laser & Photonics Reviews
Band
17
Ausgabe
12
Seite(n)
9
ISSN
1863-8880
eISSN
1863-8899
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld im Rahmen des DEAL-Vertrags gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2983726

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Kong C, Pilger C, Kunisch M, Förster C, Schulte am Esch J, Huser T. Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews . 2023;17(12):9.
Kong, C., Pilger, C., Kunisch, M., Förster, C., Schulte am Esch, J., & Huser, T. (2023). Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews , 17(12), 9. https://doi.org/10.1002/lpor.202300521
Kong, Cihang, Pilger, Christian, Kunisch, Manuel, Förster, Christine, Schulte am Esch, Jan, and Huser, Thomas. 2023. “Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser”. Laser & Photonics Reviews 17 (12): 9.
Kong, C., Pilger, C., Kunisch, M., Förster, C., Schulte am Esch, J., and Huser, T. (2023). Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews 17, 9.
Kong, C., et al., 2023. Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews , 17(12), p 9.
C. Kong, et al., “Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser”, Laser & Photonics Reviews , vol. 17, 2023, pp. 9.
Kong, C., Pilger, C., Kunisch, M., Förster, C., Schulte am Esch, J., Huser, T.: Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews . 17, 9 (2023).
Kong, Cihang, Pilger, Christian, Kunisch, Manuel, Förster, Christine, Schulte am Esch, Jan, and Huser, Thomas. “Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser”. Laser & Photonics Reviews 17.12 (2023): 9.
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2024-03-12T07:17:34Z
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