PUB - Publikationen an der Universität Bielefeld

We constructed a bifunctional fusion protein for FADH2 regeneration and successfully coexpressed it with different flavin-dependent halogenases, as well as a dioxygenase that converts 6-chlorotryptophan to 4-Cl-Kynurenine. The figure is a still life of a laboratory bench, with a single oversized E. coli bacterium hovering over an Erlenmeyer flask. Cell disruption is depicted in visual analogy to cracking an egg with the lysate appearing like egg yolk. The lysate in the flask contains all necessary enzymes for the biocatalytic cascade described in the paper rendered as ribbon structures with coloring consistent with the paper. Shown as permanent marker notes on the bench surface are a key reaction scheme as well as a “ToDo-list” that checks off some important goals of the research work.