PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions

Steensma P, Eisenhut M, Colinas M, Rosado-Souza L, Fernie AR, Weber APM, Fitzpatrick TB (2023)
Plant Physiology: kiad411.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Autor*in
Steensma, Priscille; Eisenhut, MarionUniBi ; Colinas, Maite; Rosado-Souza, Laise; Fernie, Alisdair R.; Weber, Andreas P. M.; Fitzpatrick, Teresa B.
Abstract / Bemerkung
The contribution of pyridox(am)ine 5 & PRIME;-phosphate oxidase3 (PDX3) to vitamin B6 homeostasis is an important component of C/N balance in Arabidopsis grown in nitrate and high CO2, during which it assists serine biosynthesis. The identification of factors that regulate C/N utilization in plants can make a substantial contribution to optimization of plant health. Here, we explored the contribution of pyridox(am)ine 5 & PRIME;-phosphate oxidase3 (PDX3), which regulates vitamin B-6 homeostasis, in Arabidopsis (Arabidopsis thaliana). Firstly, N fertilization regimes showed that ammonium application rescues the leaf morphological phenotype of pdx3 mutant lines but masks the metabolite perturbance resulting from impairment in utilizing soil nitrate as a source of N. Without fertilization, pdx3 lines suffered a C/N imbalance and accumulated nitrogenous compounds. Surprisingly, exploration of photorespiration as a source of endogenous N driving this metabolic imbalance, by incubation under high CO2, further exacerbated the pdx3 growth phenotype. Interestingly, the amino acid serine, critical for growth and N management, alleviated the growth phenotype of pdx3 plants under high CO2, likely due to the requirement of pyridoxal 5 & PRIME;-phosphate for the phosphorylated pathway of serine biosynthesis under this condition. Triggering of thermomorphogenesis by growth of plants at 28 & DEG;C (instead of 22 & DEG;C) did not appear to require PDX3 function, and we observed that the consequent drive toward C metabolism counters the C/N imbalance in pdx3. Further, pdx3 lines suffered a salicylic acid-induced defense response, probing of which unraveled that it is a protective strategy mediated by nonexpressor of pathogenesis related1 (NPR1) and improves fitness. Overall, the study demonstrates the importance of vitamin B-6 homeostasis as managed by the salvage pathway enzyme PDX3 to growth in diverse environments with varying nutrient availability and insight into how plants reprogram their metabolism under such conditions.
Erscheinungsjahr
2023
Zeitschriftentitel
Plant Physiology
Art.-Nr.
kiad411
ISSN
0032-0889
eISSN
1532-2548
Page URI
https://pub.uni-bielefeld.de/record/2982415

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Steensma P, Eisenhut M, Colinas M, et al. PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions. Plant Physiology. 2023: kiad411.
Steensma, P., Eisenhut, M., Colinas, M., Rosado-Souza, L., Fernie, A. R., Weber, A. P. M., & Fitzpatrick, T. B. (2023). PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions. Plant Physiology, kiad411. https://doi.org/10.1093/plphys/kiad411
Steensma, Priscille, Eisenhut, Marion, Colinas, Maite, Rosado-Souza, Laise, Fernie, Alisdair R., Weber, Andreas P. M., and Fitzpatrick, Teresa B. 2023. “PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions”. Plant Physiology: kiad411.
Steensma, P., Eisenhut, M., Colinas, M., Rosado-Souza, L., Fernie, A. R., Weber, A. P. M., and Fitzpatrick, T. B. (2023). PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions. Plant Physiology:kiad411.
Steensma, P., et al., 2023. PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions. Plant Physiology, : kiad411.
P. Steensma, et al., “PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions”, Plant Physiology, 2023, : kiad411.
Steensma, P., Eisenhut, M., Colinas, M., Rosado-Souza, L., Fernie, A.R., Weber, A.P.M., Fitzpatrick, T.B.: PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions. Plant Physiology. : kiad411 (2023).
Steensma, Priscille, Eisenhut, Marion, Colinas, Maite, Rosado-Souza, Laise, Fernie, Alisdair R., Weber, Andreas P. M., and Fitzpatrick, Teresa B. “PYRIDOX(AM)INE 5 & PRIME;-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions”. Plant Physiology (2023): kiad411.
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