Exploring Obscurin and SPEG Kinase Biology

Fleming JR, Rani A, Kraft J, Zenker S, Börgeson E, Lange S (2021)
Journal of Clinical Medicine 10(5): 984.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Fleming, Jennifer R.; Rani, Alankrita; Kraft, Jamie; Zenker, SanjaUniBi ; Börgeson, Emma; Lange, Stephan
Abstract / Bemerkung
Three members of the obscurin protein family that contain tandem kinase domains with important signaling functions for cardiac and striated muscles are the giant protein obscurin, its obscurin-associated kinase splice isoform, and the striated muscle enriched protein kinase (SPEG). While there is increasing evidence for the specific roles that each individual kinase domain plays in cross-striated muscles, their biology and regulation remains enigmatic. Our present study focuses on kinase domain 1 and the adjacent low sequence complexity inter-kinase domain linker in obscurin and SPEG. Using Phos-tag gels, we show that the linker in obscurin contains several phosphorylation sites, while the same region in SPEG remained unphosphorylated. Our homology modeling, mutational analysis and molecular docking demonstrate that kinase 1 in obscurin harbors all key amino acids important for its catalytic function and that actions of this domain result in autophosphorylation of the protein. Our bioinformatics analyses also assign a list of putative substrates for kinase domain 1 in obscurin and SPEG, based on the known and our newly proposed phosphorylation sites in muscle proteins, including obscurin itself.
Erscheinungsjahr
2021
Zeitschriftentitel
Journal of Clinical Medicine
Band
10
Ausgabe
5
Art.-Nr.
984
eISSN
2077-0383
Page URI
https://pub.uni-bielefeld.de/record/2982050

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Fleming JR, Rani A, Kraft J, Zenker S, Börgeson E, Lange S. Exploring Obscurin and SPEG Kinase Biology. Journal of Clinical Medicine. 2021;10(5): 984.
Fleming, J. R., Rani, A., Kraft, J., Zenker, S., Börgeson, E., & Lange, S. (2021). Exploring Obscurin and SPEG Kinase Biology. Journal of Clinical Medicine, 10(5), 984. https://doi.org/10.3390/jcm10050984
Fleming, Jennifer R., Rani, Alankrita, Kraft, Jamie, Zenker, Sanja, Börgeson, Emma, and Lange, Stephan. 2021. “Exploring Obscurin and SPEG Kinase Biology”. Journal of Clinical Medicine 10 (5): 984.
Fleming, J. R., Rani, A., Kraft, J., Zenker, S., Börgeson, E., and Lange, S. (2021). Exploring Obscurin and SPEG Kinase Biology. Journal of Clinical Medicine 10:984.
Fleming, J.R., et al., 2021. Exploring Obscurin and SPEG Kinase Biology. Journal of Clinical Medicine, 10(5): 984.
J.R. Fleming, et al., “Exploring Obscurin and SPEG Kinase Biology”, Journal of Clinical Medicine, vol. 10, 2021, : 984.
Fleming, J.R., Rani, A., Kraft, J., Zenker, S., Börgeson, E., Lange, S.: Exploring Obscurin and SPEG Kinase Biology. Journal of Clinical Medicine. 10, : 984 (2021).
Fleming, Jennifer R., Rani, Alankrita, Kraft, Jamie, Zenker, Sanja, Börgeson, Emma, and Lange, Stephan. “Exploring Obscurin and SPEG Kinase Biology”. Journal of Clinical Medicine 10.5 (2021): 984.
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