Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii

Perozeni F, Baier T (2023)
Life 13(7): 1566.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Perozeni, Federico; Baier, ThomasUniBi
Abstract / Bemerkung
The green model microalga Chlamydomonas reinhardtii recently emerged as a sustainable production chassis for the efficient biosynthesis of recombinant proteins and high-value metabolites. Its capacity for scalable, rapid and light-driven growth in minimal salt solutions, its simplicity for genetic manipulation and its “Generally Recognized As Safe” (GRAS) status are key features for its application in industrial biotechnology. Although nuclear transformation has typically resulted in limited transgene expression levels, recent developments now allow the design of powerful and innovative bioproduction concepts. In this review, we summarize the main obstacles to genetic engineering in C. reinhardtii and describe all essential aspects in sequence adaption and vector design to enable sufficient transgene expression from the nuclear genome. Several biotechnological examples of successful engineering serve as blueprints for the future establishment of C. reinhardtii as a green cell factory.
Erscheinungsjahr
2023
Zeitschriftentitel
Life
Band
13
Ausgabe
7
Art.-Nr.
1566
eISSN
2075-1729
Page URI
https://pub.uni-bielefeld.de/record/2980936

Zitieren

Perozeni F, Baier T. Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii. Life. 2023;13(7): 1566.
Perozeni, F., & Baier, T. (2023). Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii. Life, 13(7), 1566. https://doi.org/10.3390/life13071566
Perozeni, Federico, and Baier, Thomas. 2023. “Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii”. Life 13 (7): 1566.
Perozeni, F., and Baier, T. (2023). Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii. Life 13:1566.
Perozeni, F., & Baier, T., 2023. Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii. Life, 13(7): 1566.
F. Perozeni and T. Baier, “Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii”, Life, vol. 13, 2023, : 1566.
Perozeni, F., Baier, T.: Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii. Life. 13, : 1566 (2023).
Perozeni, Federico, and Baier, Thomas. “Current Nuclear Engineering Strategies in the Green Microalga Chlamydomonas reinhardtii”. Life 13.7 (2023): 1566.
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2023-07-15T09:43:40Z
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