Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity

Eckert S, Eilers E, Jakobs R, Anaia RA, Aragam KS, Bloss T, Popp M, Sasidharan R, Schnitzler J-P, Stein F, Steppuhn A, et al. (2023)
Metabolomics 19(7): 62.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Eckert, SilviaUniBi; Eilers, ElisabethUniBi; Jakobs, RuthUniBi; Anaia, Redouan Adam; Aragam, Kruthika Sen; Bloss, TanjaUniBi; Popp, Moritz; Sasidharan, RohitUniBi; Schnitzler, Jörg-Peter; Stein, Florian; Steppuhn, Anke; Unsicker, Sybille B.
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Abstract / Bemerkung
**Introduction**
Assessing intraspecific variation in plant volatile organic compounds (VOCs) involves pitfalls that may bias biological interpretation, particularly when several laboratories collaborate on joint projects. Comparative, inter-laboratory ring trials can inform on the reproducibility of such analyses. **Objectives**
In a ring trial involving five laboratories, we investigated the reproducibility of VOC collections with polydimethylsiloxane (PDMS) and analyses by thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS). As model plant we usedTanacetum vulgare, which shows a remarkable diversity in terpenoids, forming so-called chemotypes. We performed our ring-trial with two chemotypes to examine the sources of technical variation in plant VOC measurements during pre-analytical, analytical, and post-analytical steps. **Methods**
Monoclonal root cuttings were generated in one laboratory and distributed to five laboratories, in which plants were grown under laboratory-specific conditions. VOCs were collected on PDMS tubes from all plants before and after a jasmonic acid (JA) treatment. Thereafter, each laboratory (donors) sent a subset of tubes to four of the other laboratories (recipients), which performed TD-GC-MS with their own established procedures. **Results**
Chemotype-specific differences in VOC profiles were detected but with an overall high variation both across donor and recipient laboratories. JA-induced changes in VOC profiles were not reproducible. Laboratory-specific growth conditions led to phenotypic variation that affected the resulting VOC profiles. **Conclusion**
Our ring trial shows that despite large efforts to standardise each VOC measurement step, the outcomes differed both qualitatively and quantitatively. Our results reveal sources of variation in plant VOC research and may help to avoid systematic errors in similar experiments.
Stichworte
Thermal desorption-gas chromatography-mass spectrometry; Plant metabolites; Proficiency test; Quality assurance; Quality control; Reproducibility; Standardisation; Static headspace collection; Tansy
Erscheinungsjahr
2023
Zeitschriftentitel
Metabolomics
Band
19
Ausgabe
7
Art.-Nr.
62
eISSN
1573-3890
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld im Rahmen des DEAL-Vertrags gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2980233

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Eckert S, Eilers E, Jakobs R, et al. Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity. Metabolomics. 2023;19(7): 62.
Eckert, S., Eilers, E., Jakobs, R., Anaia, R. A., Aragam, K. S., Bloss, T., Popp, M., et al. (2023). Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity. Metabolomics, 19(7), 62. https://doi.org/10.1007/s11306-023-02026-6
Eckert, Silvia, Eilers, Elisabeth, Jakobs, Ruth, Anaia, Redouan Adam, Aragam, Kruthika Sen, Bloss, Tanja, Popp, Moritz, et al. 2023. “Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity”. Metabolomics 19 (7): 62.
Eckert, S., Eilers, E., Jakobs, R., Anaia, R. A., Aragam, K. S., Bloss, T., Popp, M., Sasidharan, R., Schnitzler, J. - P., Stein, F., et al. (2023). Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity. Metabolomics 19:62.
Eckert, S., et al., 2023. Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity. Metabolomics, 19(7): 62.
S. Eckert, et al., “Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity”, Metabolomics, vol. 19, 2023, : 62.
Eckert, S., Eilers, E., Jakobs, R., Anaia, R.A., Aragam, K.S., Bloss, T., Popp, M., Sasidharan, R., Schnitzler, J.-P., Stein, F., Steppuhn, A., Unsicker, S.B., van Dam, N.M., Yepes, S., Ziaja, D., Müller, C.: Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity. Metabolomics. 19, : 62 (2023).
Eckert, Silvia, Eilers, Elisabeth, Jakobs, Ruth, Anaia, Redouan Adam, Aragam, Kruthika Sen, Bloss, Tanja, Popp, Moritz, Sasidharan, Rohit, Schnitzler, Jörg-Peter, Stein, Florian, Steppuhn, Anke, Unsicker, Sybille B., van Dam, Nicole M., Yepes, Sol, Ziaja, Dominik, and Müller, Caroline. “Inter-laboratory comparison of plant volatile analyses in the light of intra-specific chemodiversity”. Metabolomics 19.7 (2023): 62.
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2024-07-04T08:51:55Z
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