De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant
Schilbert H, Busche M, Viehöver P, Weisshaar B, Stracke R (2023)
Bielefeld University.
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Abstract / Bemerkung
We sequenced the genome of the Arabidopsis thaliana f3h/fls1/ans triple mutant (f3h (tt6-2), Col-0 background; fls1 (fls1-2), Nö-0 background; ans (tds4-4), Col-0 background) to fully characterise its insertion alleles with Oxford Nanopore Technologies (ONT) long reads. The DNA that has been sequenced was isolated form rosette leaves. Sequencing was performed on a R9.4.1 flow cell and Basecalling was carried out with Guppy v.4.2.3. The reads were assembled with Canu v2.1.1 and the assembly is available as individual FASTA file.
Stichworte
Oxford Nanopore technology (ONT);
Arabidopsis thaliana;
Flavonol Synthase (FLS);
Anthocyanidin Synthase (ANS);
Flavanone 3-hydroxylase (F3H);
T-DNA;
transposon;
Flavonoids;
2 oxoglutarate dependent dioxygenases (2-ODDs)
Erscheinungsjahr
2023
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Page URI
https://pub.uni-bielefeld.de/record/2979807
Zitieren
Schilbert H, Busche M, Viehöver P, Weisshaar B, Stracke R. De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant . Bielefeld University; 2023.
Schilbert, H., Busche, M., Viehöver, P., Weisshaar, B., & Stracke, R. (2023). De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant . Bielefeld University. https://doi.org/10.4119/unibi/2979807
Schilbert, Hanna, Busche, Mareike, Viehöver, Prisca, Weisshaar, Bernd, and Stracke, Ralf. 2023. De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant . Bielefeld University.
Schilbert, H., Busche, M., Viehöver, P., Weisshaar, B., and Stracke, R. (2023). De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant . Bielefeld University.
Schilbert, H., et al., 2023. De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant , Bielefeld University.
H. Schilbert, et al., De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant , Bielefeld University, 2023.
Schilbert, H., Busche, M., Viehöver, P., Weisshaar, B., Stracke, R.: De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant . Bielefeld University (2023).
Schilbert, Hanna, Busche, Mareike, Viehöver, Prisca, Weisshaar, Bernd, and Stracke, Ralf. De novo ONT long read assembly of the A. thaliana f3h/fls1/ans triple mutant . Bielefeld University, 2023.
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Open Access
Zuletzt Hochgeladen
2023-06-07T09:14:44Z
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1c883d0b41fdf03b3ec0cd24269cc5db
Material in PUB:
Teil von PUB Eintrag
Generation and characterisation of an Arabidopsis thaliana f3h/fls1/ans triple mutant that accumulates eriodictyol derivatives
Schilbert H, Busche M, Sáez V, Angeli A, Weisshaar B, Martens S, Stracke R (2024)
BMC Plant Biology 24(1): 99.
Schilbert H, Busche M, Sáez V, Angeli A, Weisshaar B, Martens S, Stracke R (2024)
BMC Plant Biology 24(1): 99.
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