Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum
Schwardmann L, Wu T, Dransfeld A, Lindner SN, Wendisch VF (2023)
Applied Microbiology and Biotechnology 107(13): 4245–4260.
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| Veröffentlicht | Englisch
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Abstract / Bemerkung
Formamide is rarely used as nitrogen sourceby microorganisms. Therefore, formamide and formamidase have been used as protection system to allow for growth under non-sterile conditions and for non-sterile production of acetoin, a product lacking nitrogen. Here, we equipped Corynebacterium glutamicum, a renowned workhorse for industrial amino acid production for 60 years, with formamidase from Helicobacter pylori 26695, enabling growth with formamide as sole nitrogen source. Thereupon, the formamide/formamidase system was exploited for efficient formamide-based production of the nitrogenous compounds L-glutamate, L-lysine, N-methylphenylalanine, and dipicolinic acid by transfer of the formamide/formamidase system to established producer strains. Stable isotope labeling verified the incorporation of nitrogen from formamide into biomass and the representative product L-lysine. Moreover, we showed ammonium leakage during formamidase-based access of formamide to be exploitable to support growth of formamidase-deficient C. glutamicum in co-cultivation and demonstrated that efficient utilization of formamide as sole nitrogen source benefitted from overexpression of formate dehydrogenase.
Stichworte
Corynebacterium glutamicum;
Formamide;
Formamidase;
Amine production;
Co-cultivation
Erscheinungsjahr
2023
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
107
Ausgabe
13
Seite(n)
4245–4260
Urheberrecht / Lizenzen
eISSN
1432-0614
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Open-Access-Publikationskosten wurden durch die Universität Bielefeld im Rahmen des DEAL-Vertrags gefördert.
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https://pub.uni-bielefeld.de/record/2979137
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Schwardmann L, Wu T, Dransfeld A, Lindner SN, Wendisch VF. Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 2023;107(13):4245–4260.
Schwardmann, L., Wu, T., Dransfeld, A., Lindner, S. N., & Wendisch, V. F. (2023). Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 107(13), 4245–4260. https://doi.org/10.1007/s00253-023-12592-3
Schwardmann, Lynn, Wu, Tong, Dransfeld, Aron, Lindner, Steffen N., and Wendisch, Volker F. 2023. “Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum”. Applied Microbiology and Biotechnology 107 (13): 4245–4260.
Schwardmann, L., Wu, T., Dransfeld, A., Lindner, S. N., and Wendisch, V. F. (2023). Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum. Applied Microbiology and Biotechnology 107, 4245–4260.
Schwardmann, L., et al., 2023. Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 107(13), p 4245–4260.
L. Schwardmann, et al., “Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum”, Applied Microbiology and Biotechnology, vol. 107, 2023, pp. 4245–4260.
Schwardmann, L., Wu, T., Dransfeld, A., Lindner, S.N., Wendisch, V.F.: Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 107, 4245–4260 (2023).
Schwardmann, Lynn, Wu, Tong, Dransfeld, Aron, Lindner, Steffen N., and Wendisch, Volker F. “Formamide-based production of amines by metabolically engineering Corynebacterium glutamicum”. Applied Microbiology and Biotechnology 107.13 (2023): 4245–4260.
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Dissertation, die diesen PUB Eintrag enthält
Metabolic engineering of Corynebacterium glutamicum for sustainable xylose-based production of aromatic carboxylates and five-carbon compounds
Schwardmann LS (2023)
Bielefeld: Universität Bielefeld.
Schwardmann LS (2023)
Bielefeld: Universität Bielefeld.
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