Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii

Amendola S, Kneip JS, Meyer F, Perozeni F, Cazzaniga S, Lauersen KJ, Ballottari M, Baier T (2023)
ACS Synthetic Biology.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Amendola, Sofia; Kneip, Jacob S.; Meyer, FlorianUniBi ; Perozeni, Federico; Cazzaniga, Stefano; Lauersen, Kyle J.; Ballottari, Matteo; Baier, ThomasUniBi
Abstract / Bemerkung
Astaxanthin is a valuable ketocarotenoid with various pharmaceutical and nutraceutical applications. Green microalgae harbor natural capacities for pigment accumulation due to their 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. Recently, a redesigned ß-carotene ketolase (BKT) was found to enable ketocarotenoid accumulation in the model microalga Chlamydomonas reinhardtii, and transformants exhibited reduced photoinhibition under high-light. Here, a systematic screening by synthetic transgene design of carotenoid pathway enzymes and overexpression from the nuclear genome identified phytoene synthase (PSY/crtB) as a bottleneck for carotenoid accumulation in C. reinhardtii. Increased ß-carotene hydroxylase (CHYB) activity was found to be essential for engineered astaxanthin accumulation. A combined BKT, crtB, and CHYB expression strategy resulted in a volumetric astaxanthin production of 9.5 ± 0.3 mg L–1 (4.5 ± 0.1 mg g–1 CDW) in mixotrophic and 23.5 mg L–1 (1.09 mg L–1 h–1) in high cell density conditions, a 4-fold increase compared to previous reports in C. reinhardtii. This work presents a systematic investigation of bottlenecks in astaxanthin accumulation in C. reinhardtii and the phototrophic green cell factory design for competitive use in industrial biotechnology.
Erscheinungsjahr
2023
Zeitschriftentitel
ACS Synthetic Biology
ISSN
2161-5063
eISSN
2161-5063
Page URI
https://pub.uni-bielefeld.de/record/2969153

Zitieren

Amendola S, Kneip JS, Meyer F, et al. Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 2023.
Amendola, S., Kneip, J. S., Meyer, F., Perozeni, F., Cazzaniga, S., Lauersen, K. J., Ballottari, M., et al. (2023). Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.2c00616
Amendola, Sofia, Kneip, Jacob S., Meyer, Florian, Perozeni, Federico, Cazzaniga, Stefano, Lauersen, Kyle J., Ballottari, Matteo, and Baier, Thomas. 2023. “Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii”. ACS Synthetic Biology.
Amendola, S., Kneip, J. S., Meyer, F., Perozeni, F., Cazzaniga, S., Lauersen, K. J., Ballottari, M., and Baier, T. (2023). Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology.
Amendola, S., et al., 2023. Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology.
S. Amendola, et al., “Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii”, ACS Synthetic Biology, 2023.
Amendola, S., Kneip, J.S., Meyer, F., Perozeni, F., Cazzaniga, S., Lauersen, K.J., Ballottari, M., Baier, T.: Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. (2023).
Amendola, Sofia, Kneip, Jacob S., Meyer, Florian, Perozeni, Federico, Cazzaniga, Stefano, Lauersen, Kyle J., Ballottari, Matteo, and Baier, Thomas. “Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii”. ACS Synthetic Biology (2023).

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