Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43.
Herrera MG, Amundarain MJ, Santos J (2023)
Archives of Biochemistry and Biophysics: 109533.
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Autor*in
Herrera, Maria Georgina;
Amundarain, Maria JuliaUniBi;
Santos, Javier
Einrichtung
Abstract / Bemerkung
TDP-43 is an RNA-binding protein that presents four domains comprising an N-terminal region, two RNA recognition motifs and a C-terminal region. The N-terminal domain (NTD) has a relevant role in the oligomerization and splicing activity of TDP-43. In this work, we have expressed, purified and biophysically characterized the region that includes residues 1 to 102 that contains the nuclear localization signal (residues 80-102, NLS). Furthermore, we have evaluated the equilibrium of oligomerization for this protein fragment. Also, we have determined changes in the tertiary structure and its stability in a broad range of pH values by means of different spectroscopic methods. Additionally, we compared this fragment with the one that lacks the NLS employing experimental and computational methods. Finally, we evaluated the motion of dimeric forms to get insights into the conformational flexibility of this TDP-43 module in an oligomeric state. Our results suggest that this domain has a conformational plasticity in the vicinity of the single tryptophan of this domain (Trp68), which is enhanced by the presence of the nuclear localization signal. All these results help to understand the molecular features of the NTD of TDP-43.
Erscheinungsjahr
2023
Zeitschriftentitel
Archives of Biochemistry and Biophysics
Art.-Nr.
109533
eISSN
1096-0384
Page URI
https://pub.uni-bielefeld.de/record/2968937
Zitieren
Herrera MG, Amundarain MJ, Santos J. Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43. Archives of Biochemistry and Biophysics. 2023: 109533.
Herrera, M. G., Amundarain, M. J., & Santos, J. (2023). Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43. Archives of Biochemistry and Biophysics, 109533. https://doi.org/10.1016/j.abb.2023.109533
Herrera, Maria Georgina, Amundarain, Maria Julia, and Santos, Javier. 2023. “Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43.”. Archives of Biochemistry and Biophysics: 109533.
Herrera, M. G., Amundarain, M. J., and Santos, J. (2023). Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43. Archives of Biochemistry and Biophysics:109533.
Herrera, M.G., Amundarain, M.J., & Santos, J., 2023. Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43. Archives of Biochemistry and Biophysics, : 109533.
M.G. Herrera, M.J. Amundarain, and J. Santos, “Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43.”, Archives of Biochemistry and Biophysics, 2023, : 109533.
Herrera, M.G., Amundarain, M.J., Santos, J.: Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43. Archives of Biochemistry and Biophysics. : 109533 (2023).
Herrera, Maria Georgina, Amundarain, Maria Julia, and Santos, Javier. “Biophysical evaluation of the oligomerization and conformational properties of the N-terminal domain of TDP-43.”. Archives of Biochemistry and Biophysics (2023): 109533.
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PMID: 36740035
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