Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803
Sakurai I, Stazic D, Eisenhut M, Vuorio E, Steglich C, Hess WR, Aro E-M (2012)
Plant Physiology 160(2): 1000-1010.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Sakurai, Isamu;
Stazic, Damir;
Eisenhut, MarionUniBi
;
Vuorio, Eerika;
Steglich, Claudia;
Hess, Wolfgang R.;
Aro, Eva-Mari
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Einrichtung
Abstract / Bemerkung
The D1 protein of photosystem II in the thylakoid membrane of photosynthetic organisms is encoded by psbA genes, which in cyanobacteria occur in the form of a small gene family. Light-dependent up-regulation of psbA gene expression is crucial to ensure the proper replacement of the D1 protein. To gain a high level of gene expression, psbA transcription can be enhanced by several orders of magnitude. Recent transcriptome analyses demonstrated a high number of cis-encoded antisense RNAs (asRNAs) in bacteria, but very little is known about their possible functions. Here, we show the presence of two cis-encoded asRNAs (PsbA2R and PsbA3R) of psbA2 and psbA3 from Synechocystis sp. PCC 6803. These asRNAs are located in the 5′ untranslated region of psbA2 and psbA3 genes. Their expression becomes up-regulated by light and down-regulated by darkness, similar to their target mRNAs. In the PsbA2R-suppressing strain [PsbA2R(−)], the amount of psbA2 mRNA was only about 50% compared with the control strain. Likewise, we identified a 15% lowered activity of photosystem II and a reduced amount of the D1 protein in PsbA2R(−) compared with the control strain. The function of PsbA2R in the stabilization of psbA2 mRNA was shown from in vitro RNase E assay when the AU box and the ribosome-binding site in the 5′ untranslated region of psbA2 mRNA were both covered by PsbA2R. These results add another layer of complexity to the mechanisms that contribute to psbA gene expression and show PsbA2R as a positively acting factor to achieve a maximum level of D1 synthesis.
Erscheinungsjahr
2012
Zeitschriftentitel
Plant Physiology
Band
160
Ausgabe
2
Seite(n)
1000-1010
Urheberrecht / Lizenzen
eISSN
1532-2548
Page URI
https://pub.uni-bielefeld.de/record/2968464
Zitieren
Sakurai I, Stazic D, Eisenhut M, et al. Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803. Plant Physiology. 2012;160(2):1000-1010.
Sakurai, I., Stazic, D., Eisenhut, M., Vuorio, E., Steglich, C., Hess, W. R., & Aro, E. - M. (2012). Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803. Plant Physiology, 160(2), 1000-1010. https://doi.org/10.1104/pp.112.202127
Sakurai, Isamu, Stazic, Damir, Eisenhut, Marion, Vuorio, Eerika, Steglich, Claudia, Hess, Wolfgang R., and Aro, Eva-Mari. 2012. “Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803”. Plant Physiology 160 (2): 1000-1010.
Sakurai, I., Stazic, D., Eisenhut, M., Vuorio, E., Steglich, C., Hess, W. R., and Aro, E. - M. (2012). Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803. Plant Physiology 160, 1000-1010.
Sakurai, I., et al., 2012. Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803. Plant Physiology, 160(2), p 1000-1010.
I. Sakurai, et al., “Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803”, Plant Physiology, vol. 160, 2012, pp. 1000-1010.
Sakurai, I., Stazic, D., Eisenhut, M., Vuorio, E., Steglich, C., Hess, W.R., Aro, E.-M.: Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803. Plant Physiology. 160, 1000-1010 (2012).
Sakurai, Isamu, Stazic, Damir, Eisenhut, Marion, Vuorio, Eerika, Steglich, Claudia, Hess, Wolfgang R., and Aro, Eva-Mari. “Positive Regulation of Gene Expression by cis-Encoded Antisense RNAs in sp. PCC 6803”. Plant Physiology 160.2 (2012): 1000-1010.
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