Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions

Rademacher N, Kern R, Fujiwara T, Mettler-Altmann T, Miyagishima S-ya, Hagemann M, Eisenhut M, Weber APM (2016)
Journal of Experimental Botany 67(10): 3165-3175.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Rademacher, Nadine; Kern, Ramona; Fujiwara, Takayuki; Mettler-Altmann, Tabea; Miyagishima, Shin-ya; Hagemann, Martin; Eisenhut, MarionUniBi; Weber, Andreas P.M.
Abstract / Bemerkung
Photorespiration is essential for all organisms performing oxygenic photosynthesis. The evolution of photorespiratory metabolism began among cyanobacteria and led to a highly compartmented pathway in plants. A molecular understanding of photorespiration in eukaryotic algae, such as glaucophytes, rhodophytes, and chlorophytes, is essential to unravel the evolution of this pathway. However, mechanistic detail of the photorespiratory pathway in red algae is scarce. The unicellular red alga Cyanidioschyzon merolae represents a model for the red lineage. Its genome is fully sequenced, and tools for targeted gene engineering are available. To study the function and importance of photorespiration in red algae, we chose glycolate oxidase (GOX) as the target. GOX catalyses the conversion of glycolate into glyoxylate, while hydrogen peroxide is generated as a side-product. The function of the candidate GOX from C. merolae was verified by the fact that recombinant GOX preferred glycolate over L-lactate as a substrate. Yellow fluorescent protein-GOX fusion proteins showed that GOX is targeted to peroxisomes in C. merolae. The GOX knockout mutant lines showed a high-carbon-requiring phenotype with decreased growth and reduced photosynthetic activity compared to the wild type under ambient air conditions. Metabolite analyses revealed glycolate and glycine accumulation in the mutant cells after a shift from high CO2 conditions to ambient air. In summary, or results demonstrate that photorespiratory metabolism is essential for red algae. The use of a peroxisomal GOX points to a high photorespiratory flux as an ancestral feature of all photosynthetic eukaryotes.
Erscheinungsjahr
2016
Zeitschriftentitel
Journal of Experimental Botany
Band
67
Ausgabe
10
Seite(n)
3165-3175
ISSN
0022-0957
eISSN
1460-2431
Page URI
https://pub.uni-bielefeld.de/record/2968453

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Rademacher N, Kern R, Fujiwara T, et al. Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions. Journal of Experimental Botany. 2016;67(10):3165-3175.
Rademacher, N., Kern, R., Fujiwara, T., Mettler-Altmann, T., Miyagishima, S. -ya, Hagemann, M., Eisenhut, M., et al. (2016). Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions. Journal of Experimental Botany, 67(10), 3165-3175. https://doi.org/10.1093/jxb/erw118
Rademacher, Nadine, Kern, Ramona, Fujiwara, Takayuki, Mettler-Altmann, Tabea, Miyagishima, Shin-ya, Hagemann, Martin, Eisenhut, Marion, and Weber, Andreas P.M. 2016. “Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions”. Journal of Experimental Botany 67 (10): 3165-3175.
Rademacher, N., Kern, R., Fujiwara, T., Mettler-Altmann, T., Miyagishima, S. -ya, Hagemann, M., Eisenhut, M., and Weber, A. P. M. (2016). Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions. Journal of Experimental Botany 67, 3165-3175.
Rademacher, N., et al., 2016. Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions. Journal of Experimental Botany, 67(10), p 3165-3175.
N. Rademacher, et al., “Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions”, Journal of Experimental Botany, vol. 67, 2016, pp. 3165-3175.
Rademacher, N., Kern, R., Fujiwara, T., Mettler-Altmann, T., Miyagishima, S.-ya, Hagemann, M., Eisenhut, M., Weber, A.P.M.: Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions. Journal of Experimental Botany. 67, 3165-3175 (2016).
Rademacher, Nadine, Kern, Ramona, Fujiwara, Takayuki, Mettler-Altmann, Tabea, Miyagishima, Shin-ya, Hagemann, Martin, Eisenhut, Marion, and Weber, Andreas P.M. “Photorespiratory glycolate oxidase is essential for the survival of the red alga under ambient CO conditions”. Journal of Experimental Botany 67.10 (2016): 3165-3175.
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