A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms

Schorsch M, Kramer M, Goss T, Eisenhut M, Robinson N, Osman D, Wilde A, Sadaf S, Brückler H, Walder L, Scheibe R, et al. (2018)
Proceedings of the National Academy of Sciences 115(51): E12111-E12120.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Schorsch, Michael; Kramer, Manuela; Goss, Tatjana; Eisenhut, MarionUniBi; Robinson, Nigel; Osman, Deenah; Wilde, Annegret; Sadaf, Shamaila; Brückler, Hendrik; Walder, Lorenz; Scheibe, Renate; Hase, Toshiharu
Alle
Abstract / Bemerkung
Iron chronically limits aquatic photosynthesis, especially in marine environments, and the correct perception and maintenance of iron homeostasis in photosynthetic bacteria, including cyanobacteria, is therefore of global significance. Multiple adaptive mechanisms, responsive promoters, and posttranscriptional regulators have been identified, which allow cyanobacteria to respond to changing iron concentrations. However, many factors remain unclear, in particular, how iron status is perceived within the cell. Here we describe a cyanobacterial ferredoxin (Fed2), with a unique C-terminal extension, that acts as a player in iron perception. Fed2 homologs are highly conserved in photosynthetic organisms from cyanobacteria to higher plants, and, although they belong to the plant type ferredoxin family of [2Fe-2S] photosynthetic electron carriers, they are not involved in photosynthetic electron transport. As deletion offed2appears lethal, we developed a C-terminal truncation system to attenuate protein function. Disturbed Fed2 function resulted in decreased chlorophyll accumulation, and this was exaggerated in iron-depleted medium, where different truncations led to either exaggerated or weaker responses to low iron. Despite this, iron concentrations remained the same, or were elevated in all truncation mutants. Further analysis established that, when Fed2 function was perturbed, the classical iron limitation marker IsiA failed to accumulate at transcript and protein levels. By contrast, abundance of IsiB, which shares an operon withisiA, was unaffected by loss of Fed2 function, pinpointing the site of Fed2 action in iron perception to the level of posttranscriptional regulation.
Erscheinungsjahr
2018
Zeitschriftentitel
Proceedings of the National Academy of Sciences
Band
115
Ausgabe
51
Seite(n)
E12111-E12120
ISSN
0027-8424
eISSN
1091-6490
Page URI
https://pub.uni-bielefeld.de/record/2968439

Zitieren

Schorsch M, Kramer M, Goss T, et al. A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms. Proceedings of the National Academy of Sciences. 2018;115(51):E12111-E12120.
Schorsch, M., Kramer, M., Goss, T., Eisenhut, M., Robinson, N., Osman, D., Wilde, A., et al. (2018). A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms. Proceedings of the National Academy of Sciences, 115(51), E12111-E12120. https://doi.org/10.1073/pnas.1810379115
Schorsch, Michael, Kramer, Manuela, Goss, Tatjana, Eisenhut, Marion, Robinson, Nigel, Osman, Deenah, Wilde, Annegret, et al. 2018. “A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms”. Proceedings of the National Academy of Sciences 115 (51): E12111-E12120.
Schorsch, M., Kramer, M., Goss, T., Eisenhut, M., Robinson, N., Osman, D., Wilde, A., Sadaf, S., Brückler, H., Walder, L., et al. (2018). A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms. Proceedings of the National Academy of Sciences 115, E12111-E12120.
Schorsch, M., et al., 2018. A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms. Proceedings of the National Academy of Sciences, 115(51), p E12111-E12120.
M. Schorsch, et al., “A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms”, Proceedings of the National Academy of Sciences, vol. 115, 2018, pp. E12111-E12120.
Schorsch, M., Kramer, M., Goss, T., Eisenhut, M., Robinson, N., Osman, D., Wilde, A., Sadaf, S., Brückler, H., Walder, L., Scheibe, R., Hase, T., Hanke, G.T.: A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms. Proceedings of the National Academy of Sciences. 115, E12111-E12120 (2018).
Schorsch, Michael, Kramer, Manuela, Goss, Tatjana, Eisenhut, Marion, Robinson, Nigel, Osman, Deenah, Wilde, Annegret, Sadaf, Shamaila, Brückler, Hendrik, Walder, Lorenz, Scheibe, Renate, Hase, Toshiharu, and Hanke, Guy T. “A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms”. Proceedings of the National Academy of Sciences 115.51 (2018): E12111-E12120.
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 30514818
PubMed | Europe PMC

Suchen in

Google Scholar